FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Septin1, Uba2
FlyBase ID
FBig0000106963
Interaction Network
Interactions Browser links
Septin1 network
Uba2 network
Reported Interactions
FBrf0144953-16.Y2H
Reference
Shih et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Septin1 

Sep1

bait

fused to LexA DNA-binding domain

Septin1 
Uba2 

DmUba2

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Septin1 
N-terminal region
sufficient binding region
aa 1-325

coordinates relative to Sep1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Shih et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Shih et al., 2002)
FBrf0144953-5.Y2H
Reference
Shih et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Septin1 

Sep1

bait

fused to LexA DNA-binding domain

Uba2 

DmUba2

prey

fused to B42 activation domain

Uba2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Uba2 
C-terminal region
sufficient binding region
aa 552-700

coordinates relative to Uba2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Shih et al., 2002)

2 Uba2 clones obtained in the Sep1 screen.

(Shih et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Shih et al., 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)