FlyBase Gene Report: Dmel\Mocs3

General Information

Symbol

Dmel\Mocs3

Species

D. melanogaster

Name

Molybdenum cofactor synthesis 3

Annotation Symbol

CG13090

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032054

Gene Model Status

Current

Stock Availability

17 publicly available

Enzyme Name (EC)

molybdopterin-synthase adenylyltransferase (2.7.7.80)

thiosulfate sulfurtransferase (2.8.1.1)

molybdopterin synthase sulfurtransferase (2.8.1.11)

Gene Summary

Molybdenum cofactor synthesis 3 (Mocs3) encodes a protein involved in molybdopterin cofactor synthesis and protein urmylation. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Uba4

Key Links

Genomic Location

Cytogenetic map

29D4-29D4

Sequence location

2L:8,517,411..8,519,176 [+]

Recombination map

2-33

RefSeq locus

NT_033779 REGION:8517411..8519176

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (8 terms)

CV Term

Evidence

References

enables adenylyltransferase activity

inferred from electronic annotation with InterPro:IPR028885

(InterPro Project Members, 2004-)

enables molybdopterin-synthase adenylyltransferase activity

inferred from sequence or structural similarity with UniProtKB:P12282

(Gene Ontology Curators, 2002-)

enables molybdopterin-synthase sulfurtransferase activity

inferred from sequence or structural similarity with UniProtKB:O95396

(Gene Ontology Curators, 2002-)

enables nucleotidyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000102040

(GO Reference Genome Project, 2011-)

enables sulfurtransferase activity

inferred from electronic annotation with InterPro:IPR028885

(InterPro Project Members, 2004-)

enables thiosulfate-cyanide sulfurtransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000102040

(GO Reference Genome Project, 2011-)

enables ubiquitin-like modifier activating enzyme activity

inferred from electronic annotation with InterPro:IPR035985

(InterPro Project Members, 2004-)

enables URM1 activating enzyme activity

inferred from biological aspect of ancestor with PANTHER:PTN000102535

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in Mo-molybdopterin cofactor biosynthetic process

inferred from mutant phenotype

(Marelja et al., 2018)

involved_in protein urmylation

inferred from mutant phenotype

(Khoshnood et al., 2016)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in Mo-molybdopterin cofactor biosynthetic process

inferred from sequence or structural similarity with UniProtKB:O95396

(Gene Ontology Curators, 2002-)

involved_in molybdopterin cofactor biosynthetic process

inferred from sequence or structural similarity with UniProtKB:O95396

(Gene Ontology Curators, 2002-)

involved_in protein urmylation

inferred from biological aspect of ancestor with PANTHER:PTN000102537

(GO Reference Genome Project, 2011-)

involved_in tRNA thio-modification

inferred from sequence or structural similarity with UniProtKB:O95396

(Gene Ontology Curators, 2002-)

involved_in tRNA wobble position uridine thiolation

inferred from electronic annotation with InterPro:IPR028885

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000102537

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

is_active_in cytosol

inferred from direct assay

(Khoshnood et al., 2016)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000102040

(GO Reference Genome Project, 2011-)

located_in cytosol

inferred from electronic annotation with InterPro:IPR028885

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:O95396

(Gene Ontology Curators, 2002-)

part_of molybdopterin adenylyltransferase complex

inferred from sequence or structural similarity with UniProtKB:P12282

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

SULFURTRANSFERASES

URM1 ACTIVATING ENZYMES

UNCLASSIFIED ADENYLYLTRANSFERASES

Pathway (FlyBase)

Mo-MOLYBDOPTERIN COFACTOR BIOSYNTHESIS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

molybdopterin-synthase sulfurtransferase activity

[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [molybdopterin- synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) (2.8.1.11)

RHEA 48612: molybdopterin-synthase adenylyltransferase activity

[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate (2.7.7.80)

RHEA 43616: thiosulfate-cyanide sulfurtransferase activity

thiosulfate + hydrogen cyanide = thiocyanate + sulfite + 2 H(+) (2.8.1.1)

RHEA 16881:

Summaries

Gene Snapshot

Molybdenum cofactor synthesis 3 (Mocs3) encodes a protein involved in molybdopterin cofactor synthesis and protein urmylation. [Date last reviewed: 2019-09-26]

Gene Group (FlyBase)

SULFURTRANSFERASES -: Sulfurtransferases catalyze the transfer of sulfur-containing groups.
URM1 ACTIVATING ENZYMES -: URM1 activating enzymes are 'non-canonical' E1 enzymes that form obligate homodimers and activate the ubiquitin-like protein URM1 for subsequent steps along its conjugation cascade. (Adapted from PMID:28234446.)
UNCLASSIFIED ADENYLYLTRANSFERASES -: This group comprises adenylyltransferases that do not classify under other groups in FlyBase.

Pathway (FlyBase)

Mo-MOLYBDOPTERIN COFACTOR BIOSYNTHESIS -: Mo-molybdopterin cofactor (Moco) biosynthesis begins in the mitochondrial matrix with the production of cyclic pyranopterin phosphate (cPMP). Following its transport into the cytosol, cPMP is converted into molybdopterin by the molybdopterin synthase complex. The molybdenum (Mo) atom is then inserted forming the Mo-molybdopterin cofactor (active Moco). Moco can be incorporated as such into proteins or it can be further modified by sulfation prior to its insertion into proteins. (Adapted from FBrf0238243.)

Protein Function (UniProtKB)

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln) (By similarity). Also essential during biosynthesis of the molybdenum cofactor (PubMed:29491838). Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A (PubMed:26715182). Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus (By similarity). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards URM1 and MOCS2A (By similarity). Subsequently, a transient disulfide bond is formed (PubMed:26715182). Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions (By similarity).

(UniProt, Q9VLJ8)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mocs3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VLJ8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mocs3-RA

FBtr0079683

NM_135396

1684

453

Mocs3-RB

FBtr0333413

NM_001273326

1540

453

Mocs3-RC

FBtr0333414

NM_001273327

1687

453

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mocs3-PA

FBpp0079295

50.2

453

5.73

FBpp0305605

50.2

453

5.73

FBpp0305606

50.2

453

5.73

Q9VLJ8

NP_001260256

AGB92792

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

453 aa isoforms: Mocs3-PA, Mocs3-PB, Mocs3-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VLJ8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mocs3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.81

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in cytosol

inferred from direct assay

(Khoshnood et al., 2016)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mocs3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mocs3

View exonic expression by tissue for Dmel\Mocs3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34187/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032054

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032054

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032054

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13090

Flygut - An atlas of the Drosophila adult midgut

FBgn0032054

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mocs3

Transgenic constructs containing regulatory region of Mocs3

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel7038

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mocs3^GD7920

viable, with Scer\GAL4^Mef2.PR

Mocs3^GD7920

viable, with Scer\GAL4^pnr-MD237

Mocs3^GD7920

viable, with Scer\GAL4^tin.CΔ4

Mocs3^GD7920

Other Phenotypes

Allele

abnormal oxidative stress response, with Scer\GAL4^Act5C.PU

Mocs3^NIG.13090R

visible, with Scer\GAL4^Bx-MS1096

Mocs3^UAS.cKa

Phenotype manifest in

Allele

anterior crossvein, with Scer\GAL4^Bx-MS1096

Mocs3^UAS.cKa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

Hsap\MOCS3

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Hsap\TSTD1

1 of 14

Hsap\TSTD3

1 of 14

Hsap\TST

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Mocs3

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Uba1y

1 of 14

Rnor\Uba2

1 of 14

Rnor\Uba7

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Mocs3

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Uba1y

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\mocs3

11 of 13

Yes

Xtro\LOC116406489

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\uba2

1 of 13

Xtro\uba5

1 of 13

Xtro\XB996291

1 of 13

Yes

Danio rerio (Zebrafish) (13)

Drer\mocs3

13 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Drer\nae1

1 of 14

Drer\si:ch211-161h7.8

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\moc-3

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\MOCS3_ANOGA

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (21)

Atha\CNX5

13 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Atha\AT3G25880

1 of 13

Atha\AT5G19370

1 of 13

Atha\AT5G37530

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\UBA4

13 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Scer\TCD2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (10)

Spom\SPAC2G11.10c

12 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Yes

Spom\SPAC4H3.07c

1 of 12

Spom\tum1

1 of 12

Yes

Spom\uba5

1 of 12

Escherichia coli (enterobacterium) (8)

Ecol\moeB

10 of 11

Yes

Ecol\thiF

6 of 11

Yes

Ecol\glpE

1 of 11

Ecol\pspE

1 of 11

Ecol\sseA

1 of 11

Yes

Ecol\tcdA

1 of 11

Yes

Ecol\trhO

1 of 11

Yes

Ecol\ynjE

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mocs3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (11)

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MOCS3; molybdenum cofactor synthesis 3

14 of 14

609277

MOLYBDENUM COFACTOR DEFICIENCY, TYPE B2; MOCODB2

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mocs3 - Mocs2B

anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot

(Suganuma et al., 2012)

Mocs3 - Urm1

pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Khoshnood et al., 2016)

Mocs3 - nanos

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Bansal et al., 2020)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Mocs3

enhanceable

Urm1

(Khoshnood et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Urm1

enhanceable

Mocs3

(Khoshnood et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60307

DroID - A comprehensive database of gene and protein interactions.

FBgn0032054

MIST (protein-protein) - An integrated Molecular Interaction Database

34187

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

Mo-MOLYBDOPTERIN COFACTOR BIOSYNTHESIS

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Molybdenum cofactor biosynthesis

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Sulfur relay system

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Molybdenum cofactor biosynthesis

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-33

Cytogenetic map

29D4-29D4

Sequence location

2L:8,517,411..8,519,176 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

29D4-29D4

Limits computationally determined from genome sequence between P{lacW}l(2)k03706^k03706&P{lacW}Acer^k07704 and P{PZ}lmg⁰³⁴²⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-33

(FlyBase, 2016)

2-28.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

78827

P{UAS-Mocs3.WT}1, w^*

78828

w^*; P{UAS-Mocs3.WT}2

78829

w^*; P{UAS-Mocs3.WT}3

78830

w^*; P{UAS-Myc-Mocs3.WT}2

78831

w^*; P{UAS-Myc-Mocs3.WT}3

78832

w^*; P{UAS-EGFP-Mocs3.WT}2/CyO

78826

w^*; Mocs3ⁿ²⁹/CyO, P{ActGFP}JMR1

63997

y¹ v¹; P{TRiP.HMJ30310}attP40/CyO

NIG-Fly

13090R-1

P{NIG.13090R}1

HMJ30310

y¹ v¹; P{TRiP.HMJ30310}attP40/CyO

VDRC

v110326

P{KK100653}VIE-260B

v43558

w¹¹¹⁸; P{GD7920}v43558

More stocks available...

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (13)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT10573

Other clones

FI08414

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032054

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032054

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Khoshnood et al., 2016, Suganuma et al., 2012)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Uba4 CG13090

(Khoshnood et al., 2016)

Source for identity of: Mocs3 Uba4

(Marelja et al., 2018)

Nomenclature comments

FlyBase curator comment: Changed symbol from Uba4 to Mocs3 because this is clearly the ortholog of E.coli moeB and human MOCS3, as shown by Marelja et al. (2018).

(Marelja et al., 2018)

Etymology

Named 'Uba4' after its homologues in other species.

(FlyBase, 2009-)

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG13090

(National Institute of Genetics Fly Stocks, 2022-, Bansal et al., 2020, Otto et al., 2018, Khoshnood et al., 2016, Chang et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Suganuma et al., 2012, Wang et al., 2012, Du et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009)

Mocs3

(Marelja et al., 2018, Otto et al., 2018)

Uba4

(National Institute of Genetics Fly Stocks, 2022-, Khoshnood et al., 2016)

dMoaD

(Suganuma et al., 2012)

Name Synonyms

Molybdenum cofactor synthesis 3

Uba4 homologue

(Chang et al., 2013)

Uba4 ortholog

Ubiquitin activating enzyme 4

Ubiquitin-like activating enzyme 4

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34187

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VLJ8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VLJ8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VLJ8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34187/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032054

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032054

FlyMine - An integrated database for Drosophila genomics

FBgn0032054

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG13090

MARRVEL_MODEL - MARRVEL (model organism gene)

34187

Linkouts

BioGRID - A database of protein and genetic interactions.

60307

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032054

DroID - A comprehensive database of gene and protein interactions.

FBgn0032054

DRSC - Results frm RNAi screens

FBgn0032054

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032054

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032054

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13090

Flygut - An atlas of the Drosophila adult midgut

FBgn0032054

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032054

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC001194

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Sulfur relay system

MIST (protein-protein) - An integrated Molecular Interaction Database

34187

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Molybdenum cofactor biosynthesis

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Molybdenum cofactor biosynthesis

References (35)

The following external sites may use different assemblies or annotations than FlyBase.

3.81

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