FlyBase Allele Report: Dmel\RpS3[Plac92]

General Information

Symbol

Dmel\RpS3^Plac92

Species

D. melanogaster

Name

FlyBase ID

FBal0038901

Feature type

allele

Associated gene

Dmel\RpS3

Associated Insertion(s)

P{A92}RpS3^Plac92

Carried in Construct

Also Known As

M(3)95A(Plac92)

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Andersson et al., 1994)

Mutagen

Delta2-3 transposase

(Andersson et al., 1994)

P-element activity

(Saeboe-Larssen et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{A92}RpS3^Plac92

Cytology

Description

P{A92} insertion in the 5' UTR.

(Saeboe-Larssen et al., 1998)

P{A92} inserted between the G and T at positions -20 and -19, in the RpS3 transcription unit, between the transcription start site and the translation initiation codon.

(Andersson et al., 1994)

Allele components

Component

Use(s)

Inserted element

P{A92}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:23,358,835..23,358,836 [+]

(Levayer et al., 2015, Aoyama et al., 2013, Verma et al., 2012, Casad et al., 2011, Saeboe-Larssen et al., 1998, Andersson et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intrinsic cardiomyopathy

CEA

(Casad et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult heart

(Casad et al., 2011)

macrochaeta

(Casad et al., 2011)

scutellar bristle

(Saeboe-Larssen et al., 1998)

wing disc | larval stage

(Wada et al., 2021)

wing disc | somatic clone

(Levayer et al., 2015)

Detailed Description

Statement

Reference

There is an increase in the frequency of 'winner' clone splitting (used as a readout for winner-loser cell mixing) when cell competition is induced in the wing disc through generation of wild type clones in RpS3^Plac92/+ (a Minute mutant) tissues compared with wild type clones in wild type tissue. Clone compactness is also reduced. F-actin levels are higher in wild type clones than in RpS3^Plac92/+ cells.

(Levayer et al., 2015)

Heterozygous RpS3^Plac92 mutant females exhibit a dilated cardiomyopathy phenotype, including an increased end-systolic dimension (EDD) and a decreased fractional shortening (FS) compared to controls. Bristles appear short and thin compared to wild type (Minute phenotype).

(Casad et al., 2011)

Larval development is prolonged by about 51 hours. Female fertility (egg production rate) and vitality (fraction of hatched eggs appearing as adults) is reduced by about 45%. Scutellar bristles of heterozygotes are reduced in length and thickness by about 40%. RpS3^Plac92 is lethal in combination with RpS3^prv9 and in combination with RpS3^prv11 exhibits an extreme/semi-lethal Minute phenotype with a vitality of only 3-5%.

(Saeboe-Larssen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

RpS3^Plac92 has increased cell death | larval stage phenotype, enhanceable by Diap1[+]/Diap1⁴

(Wada et al., 2021)

RpS3^Plac92 has increased cell death | larval stage phenotype, enhanceable by Scer\GAL4^en.PU/Diap1^NIG.12284R

(Wada et al., 2021)

RpS3^Plac92 has increased cell death | larval stage phenotype, enhanceable by Scer\GAL4^nub.PU/Diap1^NIG.12284R

(Wada et al., 2021)

RpS3^Plac92 has increased cell death | larval stage phenotype, enhanceable by yki[+]/yki^B5

(Wada et al., 2021)

Suppressed by

Statement

Reference

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP}

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP}

(Wada et al., 2021)

RpS3^Plac92 has increased occurrence of cell division | larval stage phenotype, suppressible | partially by yki[+]/yki^B5

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/puc^UAS.cMa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by bsk¹/bsk[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by hep[+]/hep^r75

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by egr¹/egr[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by Tak1[+]/Tak1¹

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by grnd^GD12580/Scer\GAL4^nub.PU

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/grnd^KK109939

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/grnd^KK109939

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by hep[+]/hep^r75

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by bsk¹/bsk[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/puc^UAS.cMa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by Tak1[+]/Tak1¹

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by egr¹/egr[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by grnd^GD12580/Scer\GAL4^nub.PU

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by grnd^GD12580/Scer\GAL4^nub.PU

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/puc^UAS.cMa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by Tak1[+]/Tak1¹

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by hep[+]/hep^r75

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by bsk¹/bsk[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by egr¹/egr[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/grnd^KK109939

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype, suppressible | partially by Scer\GAL4^en.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^en.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^en.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible by Scer\GAL4^nub.PU/RpS3^UAS.cAa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible by Scer\GAL4^nub.PU/RpS3^UAS.cAa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype, suppressible by Scer\GAL4^en.PU/RpS3^UAS.cAa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype, suppressible by Scer\GAL4^en.PU/RpS3^UAS.cAa

(Wada et al., 2021)

RpS3^Plac92 has cell lethal | somatic clone phenotype, suppressible by Strica^GD12308/Scer\GAL4^en.PU/Dronc^KK104278

(Kale et al., 2015)

RpS3^Plac92 has cell lethal | somatic clone phenotype, suppressible by Scer\GAL4^en.PU/BacA\p35^UAS.cHa

(Kale et al., 2015)

RpS3^Plac92 has cell lethal | somatic clone phenotype, suppressible | partially by Scer\GAL4^en.PU/Dronc^CARD.UAS

(Kale et al., 2015)

RpS3^Plac92 has increased cell death | somatic clone phenotype, suppressible by Strica^GD12308/Scer\GAL4^en.PU/Dronc^KK104278

(Kale et al., 2015)

RpS3^Plac92 has cell lethal | somatic clone phenotype, suppressible by Scer\GAL4^en.PU/Dronc^KK104278

(Kale et al., 2015)

NOT suppressed by

Statement

Reference

RpS3^Plac92 has increased cell death | somatic clone phenotype, non-suppressible by Scer\GAL4^en.PU/Dronc^CARD.UAS

(Kale et al., 2015)

RpS3^Plac92 has cell lethal | somatic clone phenotype, non-suppressible by Strica⁴

(Kale et al., 2015)

RpS3^Plac92 has increased cell death | somatic clone phenotype, non-suppressible by Strica⁴

(Kale et al., 2015)

RpS3^Plac92 has increased cell death | somatic clone phenotype, non-suppressible by Scer\GAL4^en.PU/Dronc^KK104278

(Kale et al., 2015)

RpS3^Plac92 has increased cell death | somatic clone phenotype, non-suppressible by Strica^GD12308/Scer\GAL4^en.PU

(Kale et al., 2015)

RpS3^Plac92 has cell lethal | somatic clone phenotype, non-suppressible by Strica^GD12308/Scer\GAL4^en.PU

(Kale et al., 2015)

Enhancer of

Statement

Reference

RpS3[+]/RpS3^Plac92 is an enhancer of increased cell death | larval stage phenotype of Diap1^NIG.12284R, Scer\GAL4^nub.PU

(Wada et al., 2021)

RpS3[+]/RpS3^Plac92 is an enhancer of increased cell death | larval stage phenotype of Diap1^NIG.12284R, Scer\GAL4^en.PU

(Wada et al., 2021)

RpS3[+]/RpS3^Plac92 is an enhancer of visible | adult stage phenotype of Scer\GAL4^nub.PU, yki^KK109756

(Wada et al., 2021)

RpS3[+]/RpS3^Plac92 is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^nub.PU, yki^KK109756

(Wada et al., 2021)

NOT Suppressor of

Statement

Reference

RpS3[+]/RpS3^Plac92 is a non-suppressor of lethal | recessive | third instar larval stage phenotype of PolE1^pl10R

(Verma et al., 2012)

Other

Statement

Reference

Diap1⁴, RpS3^Plac92 has decreased size | adult stage phenotype

(Wada et al., 2021)

Diap1^NIG.12284R, RpS3^Plac92, Scer\GAL4^en.PU has visible | adult stage phenotype

(Wada et al., 2021)

Diap1^NIG.12284R, RpS3^Plac92, Scer\GAL4^en.PU has decreased size | adult stage phenotype

(Wada et al., 2021)

Diap1^NIG.12284R, RpS3^Plac92, Scer\GAL4^nub.PU has visible | adult stage phenotype

(Wada et al., 2021)

Diap1^NIG.12284R, RpS3^Plac92, Scer\GAL4^nub.PU has decreased size | adult stage phenotype

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has visible | adult stage phenotype

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has increased cell death | larval stage phenotype

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has decreased size | adult stage phenotype

(Wada et al., 2021)

Diap1⁴, RpS3^Plac92 has visible | adult stage phenotype

(Wada et al., 2021)

BacA\p35^UAS.cHa, RpS3^Plac92, Scer\GAL4^en.PU has abnormal size phenotype

(Kale et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

RpS3^Plac92 has wing disc | larval stage phenotype, enhanceable by Diap1[+]/Diap1⁴

(Wada et al., 2021)

RpS3^Plac92 has wing disc | larval stage phenotype, enhanceable by Scer\GAL4^en.PU/Diap1^NIG.12284R

(Wada et al., 2021)

RpS3^Plac92 has wing disc | larval stage phenotype, enhanceable by Scer\GAL4^nub.PU/Diap1^NIG.12284R

(Wada et al., 2021)

Suppressed by

Statement

Reference

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP}

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by Scer\GAL4^nub.PU/puc^UAS.cMa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by bsk¹/bsk[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by egr¹/egr[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by hep[+]/hep^r75

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by Tak1[+]/Tak1¹

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by grnd^GD12580/Scer\GAL4^nub.PU

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by Scer\GAL4^nub.PU/grnd^KK109939

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by Scer\GAL4^en.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by Scer\GAL4^nub.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible | partially by Scer\GAL4^en.PU/Diap1^UAS.cHa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/puc^UAS.cMa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by bsk¹/bsk[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by Tak1[+]/Tak1¹

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by grnd^GD12580/Scer\GAL4^nub.PU

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by hep[+]/hep^r75

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by Scer\GAL4^nub.PU/grnd^KK109939

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype, suppressible | partially by egr¹/egr[+]

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible by Scer\GAL4^nub.PU/RpS3^UAS.cAa

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype, suppressible by Scer\GAL4^en.PU/RpS3^UAS.cAa

(Wada et al., 2021)

Enhancer of

Statement

Reference

RpS3[+]/RpS3^Plac92 is an enhancer of wing disc | larval stage phenotype of Diap1^NIG.12284R, Scer\GAL4^nub.PU

(Wada et al., 2021)

RpS3[+]/RpS3^Plac92 is an enhancer of wing disc | larval stage phenotype of Diap1^NIG.12284R, Scer\GAL4^en.PU

(Wada et al., 2021)

RpS3[+]/RpS3^Plac92 is an enhancer of wing phenotype of Scer\GAL4^nub.PU, yki^KK109756

(Wada et al., 2021)

Other

Statement

Reference

Diap1^NIG.12284R, RpS3^Plac92, Scer\GAL4^en.PU has wing phenotype

(Wada et al., 2021)

Diap1^NIG.12284R, RpS3^Plac92, Scer\GAL4^nub.PU has wing phenotype

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing phenotype

(Wada et al., 2021)

RpS3^Plac92, yki^B5 has wing disc | larval stage phenotype

(Wada et al., 2021)

Diap1⁴, RpS3^Plac92 has wing phenotype

(Wada et al., 2021)

BacA\p35^UAS.cHa, RpS3^Plac92, Scer\GAL4^en.PU has wing disc posterior compartment phenotype

(Kale et al., 2015)

BacA\p35^UAS.cHa, RpS3^Plac92, Scer\GAL4^en.PU has wing blade posterior compartment phenotype

(Kale et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Cell death is similar in the posterior compartment expressing Dronc^{CARD.Scer\UAS} (via Scer\GAL4^en.PU) to the control anterior compartment in RpS3^Plac92/+ wing discs in which wild type clones have been induced. However, some homozygous RpS3^Plac92 cell clones are observed.

Scer\GAL4^en.PU-mediated expression of both Dronc^KK104278 and Strica^GD12308 together, but neither alone, prevents competitive cell death at RpS3^Plac92/+ boundaries with +/+ cells.

Scer\GAL4^en.PU-mediated expression of both Dronc^KK104278 and Strica^GD12308 together or Dronc^KK104278 alone, but not Strica^GD12308 alone allows survival of RpS3^Plac92 homozygous cell clones.

(Kale et al., 2015)

RpS3^Plac92 complements the lethality seen in DNApol-ε^pl10R.

(Verma et al., 2012)

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^en.PU-mediated expression of BacA\p35^Scer\UAS.cHa rescues the normal death of homozygous RpS3^Plac92 cell clones in the wing disc. In this background, homozygous RpS3^Plac92 clones generated by a 10-min heat shock (inducing 10 recombination events per compartment, on average) has no detectable effect on wing size. By contrast, homozygous RpS3^Plac92 clones generated by a 60-min heat shock (resulting in 30 'undead' clones in the posterior compartment, on average) results in a significant increase in the size of posterior compartment, detectable in the adult wings.

(Kale et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5627

w¹¹¹⁸; P{neoFRT}82B P{Ubi-GFP(S65T)nls}3R P{A92}RpS3^Plac92/TM6C, Sb¹

Kyoto

108345

w¹¹¹⁸; P{neoFRT}82B P{Ubi-GFP(S65T)nls}3R P{A92}RpS3^Plac92/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Δ2-3-induced, ry^- lines have reverted to a non-Minute phenotype.

(Andersson et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

M(3)95A(Plac92)

(Zirin and Mann, 2007, Andersson et al., 1994)

M(3)95A¹

(Saeboe-Larssen et al., 1998)

M(3)95A^Plac92

(Neufeld et al., 1998)

Plac92M(3)95A

(Lambertsson et al., 1997)

RpS3^Plac92

(Baumgartner et al., 2021, Wada et al., 2021, Cassidy et al., 2019, Akai et al., 2018, Lee et al., 2016, Verma et al., 2012, Casad et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (16)

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