Andersson, S., Saeboe-Larssen, S., Lambertsson, A., Merriam, J.R., Jacobs-Lorena, M. (1994). A Drosophila third chromosome Minute locus encodes a ribosomal protein.  Genetics 137(2): 513--520.

FBrf0072499

Research paper

Minutes (M) are a group of over 50 phenotypically similar Drosophila mutations widely believed to affect ribosomal protein genes. This report describes the characterization of the P element-induced M(3)95A(Plac92) mutation [allelic to M(3)95A]. This mutation can be reversed by the mobilization of the P element, demonstrating that the mutation is caused by insertion of this transposable element. The gene interrupted by insertion of the P element was cloned by use of inverse polymerase chain reaction. Nucleotide sequence analysis revealed a 70-75% identity to the human and rat ribosomal protein S3 genes, and to the Xenopus ribosomal protein S1a gene. At the amino acid level, the overall identity is approximately 78% for all three species. This is only the second time that a Minute has been demonstrated to encode a ribosomal protein.

PMC1205974 (PMC) (EuropePMC)