M(3)95A, M(3)w, S3, M(3R)w, dS3
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.56
Interacts with LTV1; the interaction is RNA-independent.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS3 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\RpS3 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Deletions removing RpS3 but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
Identified with: GH06471 (BDGP-DGC) <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.
Analysis of complete and partial revertants of RpS3Plac92 reveals that different degrees of RpS3 insufficiency produces distinct phenotypes, in which the penultimate effect prior to lethality constitutes arrest of gametogenesis and many morphological defects.
Calf thymus DNA exposed to γ-irradiation under N2O can be used as a means of testing for N-glycosylase activity. 8-oxoGua and FapyGua are efficiently released from the irradiated DNA substrate. The ability of RpS3 to act on base mismatches opposite 8-oxoGua and the formation of an amino intermediate are studied to characterise the N-glycosylase activity of the protein.
RpS3 is demonstrated to have dRpase activities and this suggests the ribosomal protein may be active in several steps of the DNA base excision repair pathway.
DNA repair activities are associated with RpS3 in vitro. The repair activities directed towards the mutagenic lesions 8-oxoguanine and abasic sites in DNA are able to act in vivo to protect yeast cells from H2O22 and MMS toxicity. Results suggest the possibility that RpS3 may participate in both base excision repair and protein translation.
Biochemical properties of the recombinant protein demonstrate RpS3 is capable of acting on DNA containing apurinic/apyrimidinic (AP) sites. The protein is tightly associated with the nuclear matrix suggesting the protein may have a function in DNA repair.
Cloned by hybridization to rat S3 cDNA probe. The RpS3 gene product has AP endonuclease activity.
Strong Minute; eclosion delayed 40 hours (Ferrus, 1975).
Mutant alleles have the developmental delay and bristle phenotype that makes them useful as markers in clonal analysis.
One of a class of genes (see MIN record) that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development.