Third instar larval eye-antennal imaginal disc clones homozygous for scrib1 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU do not induce significant autophagosomes (assessed by a Atg8a fluorescence reporter) in neighboring disc cells if the full organism is egr1/egr3 transheterozygous.
In an egr1 mutant background, scrib1 mutant clones in the eye-antennal disc are no longer eliminated, but grow aggressively and develop into tumours. In this background, expression of egrScer\UAS.cIa under the control of Scer\GAL4Act.PU only in the cells surrounding the scrib1 clones reverses the tumour phenotype; the scrib1 clones do not overgrow and are out-competed by the surrounding cells.
scrib1 mutant clones survive in egr1 mutant eye-antennal discs background. Overgrowth of double mutant cells is observed, and tumor development is observed in pupal eye-antennal tissue. scrib1 mutant clones generated in egr1 mutant wing discs also survive, and tumor development is observed in the wing disc.
One copy of egr1 enhances the reduction in eye size seen in both males and females when Zzzz\E4orf4Scer\UAS.cPa is expressed under the control of Scer\GAL4GMR.PF at either 24[o]C or 29[o]C. An enhancement is also seen in females at 18[o]C, but no difference is observed in males.