FlyBase Allele Report: Dmel\Rok[CAT.UAS]

General Information

Symbol

Dmel\Rok^CAT.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0122916

Feature type

allele

Associated gene

Dmel\Rok

Associated Insertion(s)

Carried in Construct

P{UAS-Rok.CAT}

Also Known As

UAS-rokCAT, UAS-Drok-CAT, UAS-Rok^CAT, UAS-Rok-CAT, UAS-rok.CA

Key Links

Transgenic product class

inframe_deletion

(Winter et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Winter et al., 2001)

Mutagen

in vitro construct

(Winter et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Rok.CAT}

Cytology

Description

UASt regulatory sequences drive expression of the catalytic domain (amino acids 1 to 454) of Rok.

(Winter et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rok

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^G12V.UAS

(Khoo et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Rok^CAT.UAS under the control of Scer\GAL4^insc-Mz1407 does not disturb the normal localization of Mira protein to a basal cortex crescent in larval brain neuroblasts during metaphase, as compared to controls.

(Koe et al., 2018)

Flies expressing Rok^CAT.Scer\UAS under the control of Scer\GAL4^nub-AC-62 have large wings compared to controls.

(Rauskolb et al., 2014)

Eye-antennal disc clones expressing Rok^GD1522 under the control of Scer\GAL4^tub.PU do not exhibit a size difference as compared to control clones, but do show increased cell death, defects in differentiation of photoreceptor cells, and cell morphology defects.

(Khoo et al., 2013)

Expression of rok^CAT.Scer\UAS in follicle cells under the control of Scer\GAL4^{Scer\FRT.Act5C} produces oocyte polarity defects.

(Sun et al., 2011)

Expression of rok^CAT.Scer\UAS under the control of Scer\GAL4^fkh.PH partially suppresses the salivary gland invagination defects of Rho1^k02107b embryos.

(Xu et al., 2008)

Expression of rok^CAT.Scer\UAS in the larval epidermal cells, under the control of Scer\GAL4^NP0056, accelerates the delamination of these cells, leading to the extrusion of more cells than in wild-type larvae.

(Ninov et al., 2007)

Expression of rok^CAT.Scer\UAS under the control of Scer\GAL4^OK107 results in truncated dorsal lobes in the mushroom body, due to axon retraction.

(Billuart et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rok^CAT.UAS, Scer\GAL4^nub-AC-62 has visible phenotype, suppressible | partially by jub^E1

(Rauskolb et al., 2014)

Rok^CAT.UAS, Scer\GAL4^Tub.PU has increased cell death | somatic clone | third instar larval stage phenotype, suppressible | partially by egr¹/egr³

(Khoo et al., 2013)

Rok^CAT.UAS, Scer\GAL4^da.G32 has lethal | pupal stage phenotype, suppressible by Mbs^N300.UAS, Scer\GAL4^da.G32

(Lee and Treisman, 2004)

NOT suppressed by

Statement

Reference

Rok^CAT.UAS, Scer\GAL4^nub-AC-62 has visible phenotype, non-suppressible by bsk^KK108156, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/Rok^CAT.UAS is a suppressor | partially of visible phenotype of mwh⁶/mwh¹

(Yan et al., 2009)

Rok^CAT.UAS, Scer\GAL4^hs.PB is a suppressor | partially of visible phenotype of Scer\GAL4^hs.PB, tow^UAS.cCa

(Chung et al., 2007)

Rok^CAT.UAS, Scer\GAL4^da.G32 is a suppressor of lethal | pupal stage phenotype of Mbs^N300.UAS, Scer\GAL4^da.G32

(Lee and Treisman, 2004)

Rok^CAT.UAS, Scer\GAL4^Bx-MS1096 is a suppressor of abnormal cytokinesis phenotype of Scer\GAL4^Bx-MS1096, pbl^{ΔDH497-549.UAS}

(Echard and O'Farrell, 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Rok^CAT.UAS is a non-suppressor of abnormal cell polarity | larval stage phenotype of vib¹³³/vib^j5A6

(Koe et al., 2018)

Rok^CAT.UAS, Scer\GAL4^nub-AC-62 is a non-suppressor of visible phenotype of Scer\GAL4^nub-AC-62, jub^RNAi.UAS.cUa

(Rauskolb et al., 2014)

Other

Statement

Reference

Ras85D^G12V.UAS, Rok^CAT.UAS, Scer\GAL4^Tub.PU has abnormal developmental rate | somatic clone | larval stage phenotype

(Khoo et al., 2013)

Ras85D^G12V.UAS, Rok^CAT.UAS, Scer\GAL4^Tub.PU has neoplasia | somatic clone | third instar larval stage phenotype

(Khoo et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rok^CAT.UAS, Scer\GAL4^Tub.PU has eye-antennal disc | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by Ras85D^G12V.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Rok^CAT.UAS, Scer\GAL4^Tub.PU has eye photoreceptor cell | third instar larval stage | somatic clone phenotype, enhanceable | somatic clone by Ras85D^G12V.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Suppressed by

Statement

Reference

Rok^CAT.UAS, Scer\GAL4^nub-AC-62 has wing phenotype, suppressible | partially by jub^E1

(Rauskolb et al., 2014)

NOT suppressed by

Statement

Reference

Rok^CAT.UAS, Scer\GAL4^nub-AC-62 has wing phenotype, non-suppressible by bsk^KK108156, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Tub.PU/Rok^CAT.UAS is a non-enhancer of eye disc | somatic clone phenotype of Mbs^T541

(Lee and Treisman, 2004)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/Rok^CAT.UAS is a suppressor | partially of wing hair | increased number phenotype of mwh⁶/mwh¹

(Yan et al., 2009)

Scer\GAL4^fkh.PH/Rok^CAT.UAS is a suppressor of presumptive embryonic salivary gland phenotype of Rho1^k02107b

(Xu et al., 2008)

Rok^CAT.UAS, Scer\GAL4^hs.PB is a suppressor | partially of wing hair | increased number phenotype of Scer\GAL4^hs.PB, tow^UAS.cCa

(Chung et al., 2007)

Rok^CAT.UAS, Scer\GAL4^Bx-MS1096 is a suppressor of wing hair | increased number phenotype of Scer\GAL4^Bx-MS1096, pbl^{ΔDH497-549.UAS}

(Echard and O'Farrell, 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Rok^CAT.UAS is a non-suppressor of larval brain | larval stage phenotype of vib¹³³/vib^j5A6

(Koe et al., 2018)

Scer\GAL4^insc-Mz1407/Rok^CAT.UAS is a non-suppressor of neuroblast | larval stage phenotype of vib¹³³/vib^j5A6

(Koe et al., 2018)

Rok^CAT.UAS, Scer\GAL4^nub-AC-62 is a non-suppressor of wing phenotype of Scer\GAL4^nub-AC-62, jub^RNAi.UAS.cUa

(Rauskolb et al., 2014)

Other

Statement

Reference

Rok^CAT.UAS, Scer\GAL4^Act.PU, l(2)gl⁴ has oocyte associated follicular epithelium | somatic clone phenotype

(Tian et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of bsk^KK108156 does not suppress the increase in wing size seen in flies expressing Rok^CAT.Scer\UAS under the control of Scer\GAL4^nub-AC-62.

Expression of Rok^CAT.Scer\UAS does not suppress the reduction in wing size seen in flies expressing jub^dsRNA.cUa under the control of Scer\GAL4^nub-AC-62.

One copy of jub^E1 slightly suppresses the increase in wing size seen in flies expressing Rok^CAT.Scer\UAS under the control of Scer\GAL4^nub-AC-62.

(Rauskolb et al., 2014)

Co-expression of Ras85D^{G12V.Scer\UAS} enhances the decreased photoreceptor differentiation seen in eye-antennal disc clones expressing Rok^CAT.Scer\UAS under the control of Scer\GAL4^tub.PU. These clones show large undifferentiated clonal masses. Larvae containing clones expressing both Ras85D^{G12V.Scer\UAS} and Rok^CAT.Scer\UAS under the control of Scer\GAL4^tub.PU exhibit an extended larval period and decrease in pupation rate, as compared with controls.

egr¹/egr³ partially suppresses the cell death observed in eye-antennal disc clones expressing Rok^CAT.Scer\UAS under the control of Scer\GAL4^tub.PU.

(Khoo et al., 2013)

Expression of rok^CAT.Scer\UAS under the control of Scer\GAL4^ptc-559.1 partially suppresses the mwh¹/mwh⁶ multiple wing hair phenotype.

(Yan et al., 2009)

The multiple wing hair phenotype caused by expression of tow^Scer\UAS.cCa under the control of Scer\GAL4^hs.PB using heat shock at 24 hours after puparium formation is partially suppressed by co-expression of rok^CAT.Scer\UAS.

(Chung et al., 2007)

Overexpression of both rok^CAT.Scer\UAS and Mbs^{N300.Scer\UAS}, under the control of Scer\GAL4^da.G32, allow ~30% of flies to survive to adulthood, instead of dying as pupae. The photoreceptor localization defect is not significantly enhanced when Mbs^T541 mutant clones express rok^CAT.Scer\UAS under the control of Scer\GAL4^tub.

(Lee and Treisman, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

6667