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General Information
Symbol
Dmel\Ras85DG12V.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0123118
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a mutated form of Ras85D (carries the amino acid replacement G12V).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eye-antennal disc clones expressing Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU do not exhibit a significant difference in the growth of clonal versus non-clonal tissue.

Primordial germ cell numbers are significantly reduced when Ras85DG12V.Scer\UAS is expressed in the primordial germ cells, under the control of Scer\GAL4C587.

Ganglionic branch 1s turn prematurely before reaching the midline in Ras85DG12V.Scer\UAS; Scer\GAL4bs-23.26 embryos.

Clones in the third instar eye disc expressing Ras85DG12V.Scer\UAS under the control of Scer\GAL4Act5C.PP that are located anterior to the morphogenetic furrow are rounded, undergo precocious neuronal development and send out axonal projections.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Other
Phenotype Manifest In
NOT suppressed by
Statement
Reference
Enhancer of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Ras85DG12V.Scer\UAS enhances the decreased photoreceptor differentiation seen in eye-antennal disc clones expressing RhoGEF2RE.Scer\UAS under the control of Scer\GAL4tub.PU. These clones show overgrowth, similar to those expressing only RhoGEF2RE.Scer\UAS, but also show large undifferentiated clonal masses in the basal regions of the posterior region of the eye disc.

Co-expression of Rac1N17.Scer\UAS, Rac1GD17411, CG11221GD7239, or diaGD9442 fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2RE.Scer\UAS and Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

The presence of Rac2Δ/+, Rac1J11/+ and MtlΔ/+ mutations fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2RE.Scer\UAS and Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

Co-expression of Ras85DG12V.Scer\UAS enhances the decreased photoreceptor differentiation seen in eye-antennal disc clones expressing RokCAT.Scer\UAS under the control of Scer\GAL4tub.PU. These clones show large undifferentiated clonal masses. Larvae containing clones expressing both Ras85DG12V.Scer\UAS and RokCAT.Scer\UAS under the control of Scer\GAL4tub.PU exhibit an extended larval period and decrease in pupation rate, as compared with controls.

Eye-antennal disc clones expressing sqhE20E21.Scer\UAS.cCa and Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU show large masses of undifferentiated tissue in the basal sections of the eye disc, and reduced differentiation of photoreceptor cells, and larvae containing these clones exhibit an extended larval period and reduced pupation rate, as compared to controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Ras85DG12V.Scer\UAS
Ras85DG12V.UAS
Name Synonyms
Secondary FlyBase IDs
    References (6)