FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\sqhE20E21.UAS.cCa
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General Information
Symbol
Dmel\sqhE20E21.UAS.cCa
Species
D. melanogaster
Name
FlyBase ID
FBal0246103
Feature type
allele
Associated gene
Dmel\sqh
Associated Insertion(s)
Carried in Construct
P{UAS-sqh.E20E21.C}
Also Known As
UAS-sqhEE, UAS-sqhE20E21
Key Links
Transgenic product class
gain_of_function_variant
(Corrigall et al., 2007)
missense_variant
(Corrigall et al., 2007)
polypeptide_post_translational_processing_variant
(Corrigall et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
gain_of_function_variant
(Corrigall et al., 2007)
missense_variant
(Corrigall et al., 2007)
polypeptide_post_translational_processing_variant
(Corrigall et al., 2007)
Mutagen
in vitro construct
(Corrigall et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-sqh.E20E21.C}
Cytology
Description

UAS regulatory sequences drive expression of a mutated form of sqh which contains two activating phosphomimetic mutations.

(Corrigall et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
sqh
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA with Ras85DG12V.UAS
      (Khoo et al., 2013)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Eye-antennal disc clones expressing sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4tub.PU do not exhibit any obvious defects in size, differentiation or cell morphology as compared to wild type clones.

      (Khoo et al., 2013)

      Expression of sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4tub.PU in clones in the anterior compartment of the eye disc induces mild cell constriction accompanied with tissue ingression.

      (Corrigall et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Inducing tension-dependent sorting (using sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4αTub84B.PBb) suppresses the contact-dependent delamination and increase in clone splitting (used as a readout for winner-loser cell mixing) seen in wild type 'loser' cells when cell competition is induced in the wing disc through generation of wild type clones in MycαTub84B.PBb-expressing wing disc tissue. No elimination is seen when tension-dependent sorting is induced in wild type tissue.

      Inducing tension-dependent sorting (using sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4αTub84B.PBb) suppresses the reduction in clone compactness seen in wild type 'loser' cells when cell competition is induced in the pupal notum through generation of wild type clones in MycαTub84B.PBb-expressing wing disc tissue.

      Inducing tension-dependent sorting (using sqhE20E21.Scer\UAS.cCa) suppresses the amount of 'loser' cell elimination seen when cell competition is induced in the wing disc through generation of clones expressing fweScer\UAS.P\T.Lose-B.N.T:Ivir\HA1 under the control of Scer\GAL4Scer\FRT.Act5C in wild type tissue.

      (Levayer et al., 2015)

      Expression of sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4GMR.PU and Scer\GAL4Pph13.1.6 suppresses the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

      (Nie et al., 2014)

      Eye-antennal disc clones expressing sqhE20E21.Scer\UAS.cCa and Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU show large masses of undifferentiated tissue in the basal sections of the eye disc, and reduced differentiation of photoreceptor cells, and larvae containing these clones exhibit an extended larval period and reduced pupation rate, as compared to controls.

      (Khoo et al., 2013)

      The ragged appearance of the midgut musculature that is seen in SNF1AΔ39 third instar larvae is rescued by expression of sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4how-24B.

      The reduced gut function seen in SNF1AΔ39 mutant larvae is rescued by expression of sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4how-24B.

      (Bland et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      sqhE20E21.Scer\UAS.cCa
      sqhE20E21.UAS.cCa
      sqhE20E21
      (Khoo et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (8)