FlyBase Allele Report: Dmel\Rac1[GD17411]

General Information

Symbol

Dmel\Rac1^GD17411

Species

D. melanogaster

Name

FlyBase ID

FBal0208220

Feature type

allele

Associated gene

Dmel\Rac1

Associated Insertion(s)

Carried in Construct

P{GD17411}

Also Known As

UAS-Rac1^RNAi, UAS-rac1-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD17411}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD17411

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{Q91.ex1.QUAS.mCherry}

(Pearce et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | larval stage, with Scer\GAL4^Hml.PG

(Howell et al., 2012)

decreased size | adult stage, with Scer\GAL4^GMR.PU

(Bosso et al., 2023)

majority die during third instar larval stage, with Scer\GAL4^Act.PU

(Bosso et al., 2023)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^GMR.PU

(Bosso et al., 2023)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Bosso et al., 2023)

Detailed Description

Statement

Reference

Larvae expressing Rac1^GD17411 under the control of Scer\GAL4^Hml.PG show strongly reduced encapsulation (10%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.

(Howell et al., 2012)

Adults expressing Rac1^GD17411 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Rac1^GD17411 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa has visible | adult stage phenotype, non-enhanceable by Vav^HMS01979, Scer\GAL4^GMR.PFa

(Ruan et al., 2016)

Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa has visible | adult stage phenotype, non-enhanceable by Rab21^JF03338, Scer\GAL4^GMR.PFa

(Ruan et al., 2016)

Suppressed by

Statement

Reference

Rac1^GD17411, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by HP1b^GD10762, Scer\GAL4^GMR.PU

(Bosso et al., 2023)

Rac1^GD17411, Scer\GAL4^GMR.PU has decreased size | adult stage phenotype, suppressible | partially by HP1b^GD10762, Scer\GAL4^GMR.PU

(Bosso et al., 2023)

NOT Enhancer of

Statement

Reference

Rac1^GD17411, Scer\GAL4^Cg.PA is a non-enhancer of increased cell number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Suppressor of

Statement

Reference

Rac1^GD17411, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

NOT Suppressor of

Statement

Reference

Rac1^GD17411, Scer\GAL4^Act.PU is a non-suppressor of majority die during third instar larval stage phenotype of HP1b^GD10762, Scer\GAL4^Act.PU

(Bosso et al., 2023)

Rac1^GD17411, Scer\GAL4^Cg.PA is a non-suppressor of increased cell number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Scer\GAL4^Tub.PU, Rac1^GD17411 is a non-suppressor | somatic clone of abnormal developmental rate | somatic clone | larval stage phenotype of Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa has eye phenotype, non-enhanceable by Vav^HMS01979, Scer\GAL4^GMR.PFa

(Ruan et al., 2016)

Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa has eye phenotype, non-enhanceable by Rab21^JF03338, Scer\GAL4^GMR.PFa

(Ruan et al., 2016)

Suppressed by

Statement

Reference

Rac1^GD17411, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by HP1b^GD10762, Scer\GAL4^GMR.PU

(Bosso et al., 2023)

NOT Enhancer of

Statement

Reference

Rac1^GD17411, Scer\GAL4^Cg.PA is a non-enhancer of embryonic/larval hemocyte | third instar larval stage | increased number phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Suppressor of

Statement

Reference

Rac1^GD17411, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

NOT Suppressor of

Statement

Reference

Rac1^GD17411, Scer\GAL4^Cg.PA is a non-suppressor of embryonic/larval hemocyte | increased number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Scer\GAL4^Tub.PU, Rac1^GD17411 is a non-suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Scer\GAL4^Tub.PU, Rac1^GD17411 is a non-suppressor | somatic clone of eye photoreceptor cell | somatic clone | third instar larval stage phenotype of Ras85D^G12V.UAS, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Other

Statement

Reference

Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa has eye disc | third instar larval stage phenotype

(Ruan et al., 2016)

Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa has early endosome | third instar larval stage phenotype

(Ruan et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is partially suppressed by co-expression of Rac1^GD17411 RNAi. The rescue of the eye phenotype cannot be made any stronger by expressing Rac1^GD17411 together with either of the following: Vav^HMS01979 RNAi, Rab21^JF03338 RNAi or Lrrk^GD11670.

Ectopic expression of Rac1^GD17411 in animals expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa disrupts the normal localization of egr protein to early endosomes in the third instar larval eye disc.

(Ruan et al., 2016)

Co-expression of Rac1^GD17411 fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2^RE.Scer\UAS and Ras85D^{G12V.Scer\UAS} under the control of Scer\GAL4^tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

(Khoo et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v49247

w¹¹¹⁸ P{GD17411}v49247

v49246

w¹¹¹⁸; P{GD17411}v49246

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rac1^GD17411

Rac1^RNAi

(Pearce et al., 2015)

rac-1^RNAi

(Scholz et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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