FlyBase Allele Report: Dmel\Gyc76C[UAS.Tag:MYC]

General Information

Symbol

Dmel\Gyc76C^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0213074

Feature type

allele

Associated gene

Dmel\Gyc76C

Associated Insertion(s)

Carried in Construct

P{UAS-Gyc76C.MYC}

Also Known As

UAS-gyc76C^WT, UAS-Gyc76C

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Ayoob et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Gyc76C.MYC}

Cytology

Description

UASt regulatory sequences drive expression of Gyc76C. The Gyc76C sequence has two Tag:MYC epitopes in place of the N-terminal signal sequence.

(Ayoob et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gyc76C

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Gyc76C^UAS.Tag:MYC under the control of Scer\GAL4^Cg.PA or Scer\GAL4^srp.D.cCa leads to an increase in the number of proliferating hemocytes and an increase in the number of hemocytes (only with Scer\GAL4^Cg.PA) in third instar larval hemolymph when compared to controls.

(Iwashita et al., 2020)

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} under the control of any of the following: Scer\GAL4^A9, Scer\GAL4^hh.PU or Scer\GAL4^en.PU results in ectopic venation in adult wings.

(Schleede and Blair, 2015)

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in all neurons, under the control of Scer\GAL4^elav.PLu, produces motor axon guidance defects in which axons often fail to defasciculate from the ISNb and ISNa axon bundles. The outermost longitudinal axon fascicle defasciculates abnormally and project axons away from the midline. Additionally, the dorsal branch of the SNa projects incorrectly between muscles 21 and 22 and then extends toward its proper target.

(Ayoob et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Gyc76C^UAS.Tag:MYC, Scer\GAL4^srp.D.cCa has increased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by Myd88^kra-1

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased cell number | third instar larval stage phenotype, non-enhanceable by Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by Pde6^UAS.EGFP, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by for^KK101298, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased cell number | third instar larval stage phenotype, non-enhanceable by Rac1^GD17411, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased cell number | third instar larval stage phenotype, non-enhanceable by Mtl^KK110274/Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Suppressed by

Statement

Reference

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased cell number | third instar larval stage phenotype, suppressible by Ras85D^GD12553, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

NOT suppressed by

Statement

Reference

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased cell number | third instar larval stage phenotype, non-suppressible by Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased cell number | third instar larval stage phenotype, non-suppressible by Mtl^KK110274/Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^srp.D.cCa has increased occurrence of cell division | third instar larval stage phenotype, non-suppressible by Myd88^kra-1

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased occurrence of cell division | third instar larval stage phenotype, non-suppressible by Pde6^UAS.EGFP, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased occurrence of cell division | third instar larval stage phenotype, non-suppressible by for^KK101298, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has increased cell number | third instar larval stage phenotype, non-suppressible by Rac1^GD17411, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

NOT Suppressor of

Statement

Reference

Gyc76C^UAS.Tag:MYC/Scer\GAL4^en.PU is a non-suppressor of visible phenotype of cv⁷⁰

(Schleede and Blair, 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Gyc76C^UAS.Tag:MYC, Scer\GAL4^srp.D.cCa has embryonic/larval hemocyte | third instar larval stage phenotype, non-enhanceable by Myd88^kra-1

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | third instar larval stage | increased number phenotype, non-enhanceable by Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | third instar larval stage | increased number phenotype, non-enhanceable by Pde6^UAS.EGFP, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | third instar larval stage | increased number phenotype, non-enhanceable by for^KK101298, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | third instar larval stage | increased number phenotype, non-enhanceable by Rac1^GD17411, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | third instar larval stage | increased number phenotype, non-enhanceable by Mtl^KK110274/Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Suppressed by

Statement

Reference

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | increased number | third instar larval stage phenotype, suppressible by Ras85D^GD12553, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

NOT suppressed by

Statement

Reference

Gyc76C^UAS.Tag:MYC, Scer\GAL4^srp.D.cCa has embryonic/larval hemocyte | third instar larval stage phenotype, non-suppressible by Myd88^kra-1

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | increased number | third instar larval stage phenotype, non-suppressible by Pde6^UAS.EGFP, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | increased number | third instar larval stage phenotype, non-suppressible by for^KK101298, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | increased number | third instar larval stage phenotype, non-suppressible by Rac1^GD17411, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | increased number | third instar larval stage phenotype, non-suppressible by Mtl^KK110274/Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA has embryonic/larval hemocyte | increased number | third instar larval stage phenotype, non-suppressible by Rac2^GD17536, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

NOT Suppressor of

Statement

Reference

Gyc76C^UAS.Tag:MYC/Scer\GAL4^en.PU is a non-suppressor of wing blade phenotype of cv⁷⁰

(Schleede and Blair, 2015)

Gyc76C^UAS.Tag:MYC/Scer\GAL4^en.PU is a non-suppressor of posterior crossvein phenotype of cv⁷⁰

(Schleede and Blair, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The complete loss of the wing posterior crossvein in cv⁷⁰ hemizygous adults cannot be reverted by expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en.PU (which on its own leads to ectopic venation).

(Schleede and Blair, 2015)

Coexpression of Gyc76C^{Scer\UAS.T:Hsap\MYC} with plexA^Scer\UAS.cWa, under the control of Scer\GAL4^elav.PLu, enhances the axon guidance phenotype of plexA^Scer\UAS.cWa-expressing embryos, with many axon bundles crossing the midline.

(Ayoob et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Gyc76C^UAS.Tag:MYC/Scer\GAL4^c564 rescues Gyc76C^KG03723

(Kanoh et al., 2021)

Scer\GAL4^fkh.PH/Gyc76C^UAS.Tag:MYC rescues Gyc76C²³⁸⁸

(Patel and Myat, 2013)

Gyc76C^UAS.Tag:MYC/Scer\GAL4^twi.PB rescues Gyc76C²³⁸⁸

(Patel and Myat, 2013)

Scer\GAL4^fkh.PH, Gyc76C^UAS.Tag:MYC, Scer\GAL4^twi.PB rescues Gyc76C²³⁸⁸

(Patel and Myat, 2013)

Partially rescues

Scer\GAL4^Hml.PG/Gyc76C^UAS.Tag:MYC partially rescues Gyc76C^KG03723

(Iwashita et al., 2020)

Gyc76C^UAS.Tag:MYC, Scer\GAL4^twi.PB, Scer\GAL4^Mef2.PR partially rescues Gyc76C²³⁸⁸

(Patel et al., 2012)

Gyc76C^UAS.Tag:MYC/Scer\GAL4^elav.PLu partially rescues Gyc76C^KG03723ex173

(Ayoob et al., 2004)

Comments

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in either the salivary glands or mesoderm, under the control of Scer\GAL4^fkh.PH or Scer\GAL4^twi.PB significantly rescues the gland migration defect found in Gyc76C²³⁸⁸ homozygous embryos. Simultaneous expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in the salivary gland and mesoderm does not result in increased rescue of the migration defect compared to expression in either tissue alone. In contrast to the rescue of the migration defects, the salivary gland invagination defects of Gyc76C²³⁸⁸ mutant embryos are better rescued by expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in the gland than in the mesoderm.

(Patel and Myat, 2013)

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} under the simultaneous control of both Scer\GAL4^Mef2.PR and Scer\GAL4^twi.PB partially rescues the somatic muscle defects of Gyc76C²³⁸⁸ homozygous embryos, such that only 10% of stage 14 and 5% of stage 16 embryos show a patterning defect.

(Patel et al., 2012)

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in all neurons under the control of Scer\GAL4^elav.PLu significantly, but not completely, rescues the motor axon guidance and lethality phenotypes of Gyc76C^KG03723ex173 embryos.

(Ayoob et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66162

w^*; P{UAS-Gyc76C.MYC}1/CyO, P{act-lacZ.B}CB1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Gyc76C^{Scer\UAS.T:Hsap\MYC}

Gyc76C^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (6)

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