FlyBase Allele Report: Dmel\Gyc76C[KG03723ex173]

General Information

Symbol

Dmel\Gyc76C^KG03723ex173

Species

D. melanogaster

Name

FlyBase ID

FBal0213072

Feature type

allele

Associated gene

Dmel\Gyc76C

Associated Insertion(s)

Carried in Construct

Also Known As

gyc76C^Ex173

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Ayoob et al., 2004)

Mutagen

Delta2-3 transposase

(Ayoob et al., 2004)

Progenitor genotype

P{SUPor-P}Gyc76C^KG03723

(Ayoob et al., 2004)

Cytology

Description

Imprecise excision of the P{SUPor-P} element, resulting in deletion of ~8kb of genomic sequence including a Gyc76C exon.

(Ayoob et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Gyc76C^KG03723ex173/+ embryos exhibit an increase in embryonic ISNb motor axon defects as compared to controls.

Gyc76C^KG03723ex173/+ and Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 embryos show no apparent differences in muscle development or organization.

Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 embryos exhibit abnormal motor axon pathways in both ISNb and SNa motor neurons.

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 homozygous and Gyc76C^KG03723ex173/Gyc76C²³⁸⁸ heterozygous embryos display a severe salivary gland migration defect where mutant gland fail to turn completely and some glands appear branched or folded.

(Patel and Myat, 2013)

Patterning of the ventral and lateral somatic muscles is abnormal in homozygous and Gyc76C^KG03723ex173/Gyc76C²³⁸⁸ embryos, whereas dorsal muscles are patterned normally.

(Patel et al., 2012)

Gyc76C^KG03723ex173 embryos show motor axon guidance defects as ISNb and SNa axons often fail to defasciculate and do not innervate their proper muscle targets. Gyc76C^KG03723ex33/Gyc76C^KG03723ex173 and Gyc76C^KG03723ex173/Df(3L)5126 embryos show the same phenotype. Gyc76C^KG03723/+ heterozygotes show a milder version of this phenotype.

(Ayoob et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gyc76C^KG03723ex173 has abnormal neuroanatomy | dominant phenotype, enhanceable by Sema1a^k13702/Sema-1a[+]

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has abnormal neuroanatomy | dominant phenotype, enhanceable by Df(4)C3/+

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has abnormal neuroanatomy | dominant phenotype, enhanceable by Df(3R)swp2^MICAL/+

(Ayoob et al., 2004)

NOT Enhanced by

Statement

Reference

Gyc76C^KG03723ex173 has abnormal neuroanatomy | dominant phenotype, non-enhanceable by ena[+]/ena^GC10

(Ayoob et al., 2004)

NOT suppressed by

Statement

Reference

Gyc76C^KG03723ex173 has abnormal neuroanatomy | dominant phenotype, non-suppressible by ena[+]/ena^GC10

(Ayoob et al., 2004)

Enhancer of

Statement

Reference

Gyc76C[+]/Gyc76C^KG03723ex173 is an enhancer of abnormal neuroanatomy | embryonic stage | semidominant phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

NOT Enhancer of

Statement

Reference

Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 is a non-enhancer of abnormal neuroanatomy | embryonic stage | semidominant phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

Suppressor of

Statement

Reference

Gyc76C[+]/Gyc76C^KG03723ex173 is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^Mef2.PR, Sema1a^UAS.cYa

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is a suppressor | partially of abnormal neuroanatomy phenotype of PlexA^UAS.cWa, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gyc76C^KG03723ex173 has larval intersegmental nerve phenotype, enhanceable by Sema1a^k13702/Sema-1a[+]

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has larval abdominal segmental nerve phenotype, enhanceable by Sema1a^k13702/Sema-1a[+]

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has larval intersegmental nerve phenotype, enhanceable by Df(4)C3/+

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has larval abdominal segmental nerve phenotype, enhanceable by Df(4)C3/+

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has larval intersegmental nerve phenotype, enhanceable by Df(3R)swp2^MICAL/+

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has larval abdominal segmental nerve phenotype, enhanceable by Df(3R)swp2^MICAL/+

(Ayoob et al., 2004)

NOT Enhanced by

Statement

Reference

Gyc76C^KG03723ex173 has larval abdominal segmental nerve phenotype, non-enhanceable by ena[+]/ena^GC10

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has larval intersegmental nerve phenotype, non-enhanceable by ena[+]/ena^GC10

(Ayoob et al., 2004)

NOT suppressed by

Statement

Reference

Gyc76C^KG03723ex173 has larval intersegmental nerve phenotype, non-suppressible by ena[+]/ena^GC10

(Ayoob et al., 2004)

Gyc76C^KG03723ex173 has larval abdominal segmental nerve phenotype, non-suppressible by ena[+]/ena^GC10

(Ayoob et al., 2004)

Enhancer of

Statement

Reference

Gyc76C[+]/Gyc76C^KG03723ex173 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

Gyc76C[+]/Gyc76C^KG03723ex173 is an enhancer of larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

Gyc76C[+]/Gyc76C^KG03723ex173 is an enhancer of axon | embryonic stage phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

NOT Enhancer of

Statement

Reference

Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 is a non-enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 is a non-enhancer of larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 is a non-enhancer of axon | embryonic stage phenotype of kermit^ex31

(Chak and Kolodkin, 2014)

Suppressor of

Statement

Reference

Gyc76C[+]/Gyc76C^KG03723ex173 is a suppressor | partially of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Scer\GAL4^Mef2.PR, Sema1a^UAS.cYa

(Chak and Kolodkin, 2014)

Gyc76C[+]/Gyc76C^KG03723ex173 is a suppressor | partially of axon | embryonic stage phenotype of Scer\GAL4^Mef2.PR, Sema1a^UAS.cYa

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is a suppressor | partially of fascicle | ectopic phenotype of PlexA^UAS.cWa, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

kermit^ex31/+, Gyc76C^KG03723ex173/+ double mutants exhibit an increased number of defects in both ISNb and SNa motor neuron axon pathways as compared to kermit^ex31/+ mutant embryos.

kermit^ex31/kermit^ex31, Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 double mutant embryos exhibit a similar number of defects in ISNb motor neuron pathways as compared to kermit^ex31/kermit^ex31 or Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 single mutant embryos, and a similar number of defects in SNa motor neuron pathways as compared to kermit^ex31/kermit^ex31 single mutant embryos.

The ISNb axon pathfinding, positioning and bypass defects caused by expression of Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^Mef2.PR are significantly reduced in Gyc76C^KG03723ex173/+ embryos.

(Chak and Kolodkin, 2014)

Sema-1a^k13702/+; Gyc76C^KG03723ex173/+ double mutant embryos show a motor axon guidance phenotype that is similar in severity to Gyc76C^KG03723ex173 homozygotes, while Gyc76C^KG03723ex173 heterozygotes show a much milder phenotype. One copy of either Df(4)C3 or Df(3R)swp2^MICAL also enhances the axon guidance phenotype of Gyc76C^KG03723ex173/+ embryos.

The Gyc76C^KG03723ex173/+ phenotype is not modified in ena^GC10/+; Gyc76C^KG03723ex173/+ double mutants.

A Gyc76C^KG03723ex173 background partially suppresses the aberrant axon midline-crossing phenotype of embryos that express plexA^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu.

(Ayoob et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Gyc76C^KG03723ex173 is partially rescued by Scer\GAL4^elav.PU/Gyc76C^{UAS.FL.Tag:MYC}

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is partially rescued by Gyc76C^UAS.Tag:MYC/Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Not rescued by

Gyc76C^KG03723ex173 is not rescued by Scer\GAL4^elav.PU/Gyc76C^{ΔEcto.UAS.Tag:MYC}

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is not rescued by Scer\GAL4^elav.PU/Gyc76C^{ΔKHD.UAS.Tag:MYC}

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is not rescued by Scer\GAL4^elav.PU/Gyc76C^{ΔDD.UAS.Tag:MYC}

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is not rescued by Scer\GAL4^elav.PU/Gyc76C^{ΔCterm.UAS.Tag:MYC}

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is not rescued by Scer\GAL4^elav.PU/Gyc76C^{ΔEndo.UAS.Tag:MYC}

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is not rescued by Scer\GAL4^elav.PU/Gyc76C^{ΔPBM.UAS.Tag:MYC}

(Chak and Kolodkin, 2014)

Gyc76C^KG03723ex173 is not rescued by Gyc76C^{D945A.UAS.Tag:MYC}/Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Comments

Expression of Gyc76C^{Scer\UAS.FL.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU partially rescues the ISNb and SNa motor neuron axon pathway phenotypes of Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 embryos.

Expression of Gyc76C^{ΔEcto.Scer\UAS.T:Hsap\MYC}, Gyc76C^{ΔKHD.Scer\UAS.T:Hsap\MYC}, Gyc76C^{ΔDD.Scer\UAS.T:Hsap\MYC}, Gyc76C^{ΔCterm.Scer\UAS.T:Hsap\MYC}, Gyc76C^{ΔEndo.Scer\UAS.T:Hsap\MYC}, or Gyc76C^{ΔPBM.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU fails to rescue the ISNb and SNa motor neuron axon pathway phenotypes of Gyc76C^KG03723ex173/Gyc76C^KG03723ex173 embryos.

(Chak and Kolodkin, 2014)

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in either the salivary glands or mesoderm, under the control of Scer\GAL4^fkh.PH or Scer\GAL4^twi.PB significantly rescues the gland migration defect found in Gyc76C²³⁸⁸ homozygous embryos. Simultaneous expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in the salivary gland and mesoderm does not result in increased rescue of the migration defect compared to expression in either tissue alone.

(Patel and Myat, 2013)

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in all neurons under the control of Scer\GAL4^elav.PLu significantly, but not completely, rescues the motor axon guidance and lethality phenotypes of Gyc76C^KG03723ex173 embryos.

(Ayoob et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

65741

w^*; Gyc76C^KG03723ex173/TM3, P{act-lacZ.B}JT1, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gyc76C^KG03723ex173

Gyc76C^ex173

(Chak and Kolodkin, 2014, Patel and Myat, 2013)

gyc76C^Ex173

(Patel et al., 2012)

gyc76C^KG03723ex173

(Ayoob et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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