FlyBase Allele Report: Dmel\Rab21[JF03338]

General Information

Symbol

Dmel\Rab21^JF03338

Species

D. melanogaster

Name

FlyBase ID

FBal0242511

Feature type

allele

Associated gene

Dmel\Rab21

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03338}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03338}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03338

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Rab21^JF03338 RNAi under the control of Scer\GAL4^GMR.PFa does not perturb the normal adult eye structure or appearance.

(Ruan et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Rab21^JF03338, Scer\GAL4^GMR.PFa is a non-enhancer of visible | adult stage phenotype of Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Suppressor of

Statement

Reference

Rab21^JF03338, Scer\GAL4^ptc.PU is a suppressor | partially of increased mortality during development phenotype of Hsap\KIF5B::Hsap\RET^UAS.cDa, Scer\GAL4^ptc.PU

(Das and Cagan, 2017)

Rab21^JF03338, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Other

Statement

Reference

Hsap\KIF5B::Hsap\RET^UAS.cDa, Rab21^JF03338, Scer\GAL4^ptc.PU has partially lethal - majority die phenotype

(Das and Cagan, 2017)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Rab21^JF03338, Scer\GAL4^GMR.PFa is a non-enhancer of eye phenotype of Rac1^GD17411, Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Suppressor of

Statement

Reference

Rab21^JF03338, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Other

Statement

Reference

Rab21^JF03338, Scer\GAL4^GMR.PFa, egr^UAS.cMa has eye disc | third instar larval stage phenotype

(Ruan et al., 2016)

Rab21^JF03338, Scer\GAL4^GMR.PFa, egr^UAS.cMa has early endosome | third instar larval stage phenotype

(Ruan et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is partially suppressed by co-expression of Rac1^GD17411 RNAi. The rescue of the eye phenotype cannot be made any stronger by expressing Rac1^GD17411 together with Rab21^JF03338 RNAi.

Expression of Rab21^JF03338 RNAi in animals expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa leads to reduced localization of egr to early endosomes in third instar larval eye disc.

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is partially suppressed by co-expression of Rab21^JF03338 RNAi. The rescue of the eye phenotype cannot be made any stronger by expressing Rab21^JF03338 RNAi together with Lrrk^GD11670 RNAi.

(Ruan et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29403

y¹ v¹; P{TRiP.JF03338}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rab21^JF03338

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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