FlyBase Allele Report: Dmel\bsk[KK108156]

General Information

Symbol

Dmel\bsk^KK108156

Species

D. melanogaster

Name

FlyBase ID

FBal0235389

Feature type

allele

Associated gene

Dmel\bsk

Associated Insertion(s)

Carried in Construct

P{KK108156}

Also Known As

UAS-bsk-RNAi, bsk RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108156}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108156

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response | adult stage, with Scer\GAL4^repo

(Hsu et al., 2021)

majority die during P-stage, with Scer\GAL4^Tub.PU

(Havula et al., 2022)

partially lethal - majority die, with Scer\GAL4^Tub.PU

(Havula et al., 2022)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^repo

(Hsu et al., 2021)

wing nerve, with Scer\GAL4^repo

(Hsu et al., 2021)

Detailed Description

Statement

Reference

Expression of bsk^KK108156 RNAi under the control Scer\GAL4^Hml.Δ driver significantly improves the lowered survival of adult flies on lard-supplemented diet compared to controls but does not affect plasmatocyte count in adult flies.

(Woodcock et al., 2015)

Expression of bsk^KK108156 in the posterior compartment of the imaginal wing disc, under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU does not affect levels of cell death.

(Wong et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

BubR1^RNAi.UAS.cUa, Scer\GAL4^hh.PU, bsk^KK108156 has increased cell death phenotype, suppressible by dap^UAS.cLa, Scer\GAL4^hh.PU

(Wong et al., 2014)

BubR1^RNAi.UAS.cUa, Scer\GAL4^en.PU, bsk^KK108156 has increased cell death phenotype, suppressible by dap^UAS.cLa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Dark^RNAi.UAS.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Dark^RNAi.UAS.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by hid^RNAi.UAS.cUa/Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by hid^RNAi.UAS.cUa/Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Debcl^RNAi.UAS.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Debcl^RNAi.UAS.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by CycB[+]/CycB²

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by CycB[+]/CycB²

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Myt1^UAS.cPa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Myt1^UAS.cPa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

NOT Enhancer of

Statement

Reference

Scer\GAL4^ple.PF/bsk^KK108156 is a non-enhancer of chemical resistant phenotype of Scer\GAL4^ple.PF, mth^KK106399

(Shukla et al., 2014)

Scer\GAL4^ple.PF/bsk^KK108156 is a non-enhancer of abnormal oxidative stress response phenotype of Scer\GAL4^ple.PF, mth^KK106399

(Shukla et al., 2014)

Suppressor of

Statement

Reference

bsk^KK108156/Scer\GAL4^GMR.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cMa

(Velentzas et al., 2018)

bsk^KK108156/Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cMa

(Velentzas et al., 2018)

bsk^KK108156, Scer\GAL4^nub.PU is a suppressor | partially of visible | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

bsk^KK108156, Scer\GAL4^nub.PU is a suppressor | partially of abnormal size | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

bsk^KK108156, Scer\GAL4^nub.PU is a suppressor of increased cell death | third instar larval stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

bsk^KK108156/Scer\GAL4^rn-GAL4-5 is a suppressor of neoplasia phenotype of Scer\GAL4^rn-GAL4-5, Syx7^GD2767

(Andersen et al., 2015)

NOT Suppressor of

Statement

Reference

bsk^KK108156, Scer\GAL4^nub-AC-62 is a non-suppressor of visible phenotype of Rok^CAT.UAS, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

Scer\GAL4^ple.PF/bsk^KK108156 is a non-suppressor of chemical resistant phenotype of Scer\GAL4^ple.PF, mth^KK106399

(Shukla et al., 2014)

Scer\GAL4^ple.PF/bsk^KK108156 is a non-suppressor of abnormal oxidative stress response phenotype of Scer\GAL4^ple.PF, mth^KK106399

(Shukla et al., 2014)

Other

Statement

Reference

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

BubR1^RNAi.UAS.cUa, Scer\GAL4^hh.PU, bsk^KK108156 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

BubR1^RNAi.UAS.cUa, Scer\GAL4^en.PU, bsk^KK108156 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Debcl^RNAi.UAS.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Debcl^RNAi.UAS.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

BubR1^RNAi.UAS.cUa, Scer\GAL4^hh.PU, bsk^KK108156 has wing disc phenotype, suppressible by dap^UAS.cLa, Scer\GAL4^hh.PU

(Wong et al., 2014)

BubR1^RNAi.UAS.cUa, Scer\GAL4^en.PU, bsk^KK108156 has wing disc phenotype, suppressible by dap^UAS.cLa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Dark^RNAi.UAS.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Dark^RNAi.UAS.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by hid^RNAi.UAS.cUa/Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by hid^RNAi.UAS.cUa/Scer\GAL4^en.PU

(Wong et al., 2014)

Suppressor of

Statement

Reference

bsk^KK108156/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cMa

(Velentzas et al., 2018)

bsk^KK108156, Scer\GAL4^nub.PU is a suppressor | partially of wing phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

bsk^KK108156, Scer\GAL4^nub.PU is a suppressor of wing disc | third instar larval stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

NOT Suppressor of

Statement

Reference

bsk^KK108156, Scer\GAL4^nub-AC-62 is a non-suppressor of wing phenotype of Rok^CAT.UAS, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

Other

Statement

Reference

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

BubR1^RNAi.UAS.cUa, Scer\GAL4^hh.PU, bsk^KK108156 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

BubR1^RNAi.UAS.cUa, Scer\GAL4^en.PU, bsk^KK108156 has wing disc phenotype

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The extensive cell death in the pouch region of wing discs in third instar larvae as well as the small and deformed wings in adults expressing His1:CG31617^NIG.31617R under the control of Scer\GAL4^nub.PU are significantly suppressed by co-expression of bsk^KK108156.

(Bayona-Feliu et al., 2017)

Expression of bsk^KK108156 does not suppress the increase in wing size seen in flies expressing Rok^CAT.Scer\UAS under the control of Scer\GAL4^nub-AC-62.

(Rauskolb et al., 2014)

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when bsk^KK108156 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This leads to an increase in cell death. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

A BubR1 deficient background (that leads to significant chromosomal instability), created through expression of BubR1^VDRC.cUa under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU, leads to a significant increase in cell death in bsk^KK108156 knockdown flies.

Overexpression of dap^Scer\UAS.cLa in the imaginal discs greatly reduces the amount of apoptosis seen in bsk^KK108156 and BubR1^VDRC.cUa knockdown cells (with all transgenes under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU).

Knockdown of Dark through expression of Dark^VDRC.cUa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU results in significantly reduced levels of cell death in imaginal discs expressing bsk^KK108156 and mad2^GD16427.

Knockdown of hid through expression of hid^VDRC.cUa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU results in significantly reduced levels of cell death in imaginal discs expressing bsk^KK108156 and mad2^GD16427.

Knockdown of Debcl through expression of Debcl^VDRC.cUa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU results in significantly reduced levels of cell death in imaginal discs expressing bsk^KK108156 and mad2^GD16427.

Cell death induced by bsk knockdown in cells exhibiting chromosomal instability (through expression of mad2^GD16427) is reduced by lengthening G[[2]] through a CycB² heterozygous background.

Cell death induced by bsk knockdown in cells exhibiting chromosomal instability (through expression of mad2^GD16427) is reduced by lengthening G[[2]] by Myt1^Scer\UAS.cPa overexpression.

(Wong et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of bsk^KK108156 in the posterior wing disc compartment of flies expressing mad2^GD16427 (with both lines under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU) results in both chromosome instability and high levels of cell death. This cell death is inhibited by the addition of BacA\p35^Scer\UAS.cHa.

(Wong et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v104569

P{KK108156}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

JNK^RNAi

(Chen et al., 2020)

bsk^KK108156

bsk^RNAi

(Bayona-Feliu et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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