FlyBase Allele Report: Dmel\jub[E1]

General Information

Symbol

Dmel\jub^E1

Species

D. melanogaster

Name

FlyBase ID

FBal0283114

Feature type

allele

Associated gene

Dmel\jub

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Sabino et al., 2011)

Progenitor genotype

jub^EPG845

(Sabino et al., 2011)

Cytology

Description

Mobilisation of P{EP}jub^EPG845 generates a 1.242kb deletion of the X chromosome (spanning from 13724163 to 13725403bp).

(Sabino et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | somatic clone

(Sabino et al., 2011)

decreased cell growth rate | somatic clone

(Sabino et al., 2011)

lethal - all die before end of prepupal stage

(Sabino et al., 2011)

some die during prepupal stage | recessive

(Sabino et al., 2011)

Phenotype Manifest In

centrosome | somatic clone

(Sabino et al., 2011)

neuroblast | somatic clone

(Sabino et al., 2011)

spindle | somatic clone

(Sabino et al., 2011)

Detailed Description

Statement

Reference

Heterozygosity for jub^E1 has little effect on wing size.

(Rauskolb et al., 2014)

jub^E1 mutants survive to early pupal stages, even when the maternal contribution is removed by generating germline clones. jub^E1 mutant clones within otherwise wild-type imaginal discs grow poorly and delaminate from the epithelia. However, cell-cell adhesion appears fine and there are no polarity defects, as reflected by the normal distribution of shg.

Mitotic cells in the brains of jub^E1 homozygous mutant larvae exhibit a range of defects: 10% lack detectable centrosomes, 30% have a single centrosome, 20% have two centrosomes that are unusually close to each other and mis-position at the cell centre, while the remaining 40% are wild-type. In 17% of jub^E1 mutant neuroblasts the distance between centrosomes in well-established bipolar spindles is very small, which is never the case in wild-type.

jub^E1 mutant neuroblasts exhibit mitotic spindle defects, even in neuroblasts with two well-separated centrosomes. These include bipolar anastral spindles and completely disorganised spindles, which appear to be nucleated byt the chromatin pathway.

Approximately 11% of jub^E1 mutant neuroblasts exhibit abnormal spindle orientation. However, quantification of neuroblast numbers suggests that the defects in spindle positioning and cortical targeting of cell-fate determinants are corrected as the cells exit mitosis.

(Sabino et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

jub^E1 has abnormal mitotic cell cycle | somatic clone phenotype, enhanceable by aurA^Ubi-p63E.GFP

(Sabino et al., 2011)

Suppressed by

Statement

Reference

jub^E1 has abnormal mitotic cell cycle | somatic clone phenotype, suppressible by aurA^87Ac-3/aur[+]

(Sabino et al., 2011)

Enhancer of

Statement

Reference

jub^E1 is an enhancer of visible phenotype of Rok^RNAi.UAS.cUa, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

Suppressor of

Statement

Reference

jub^E1 is a suppressor | partially of visible phenotype of Rok^CAT.UAS, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

Other

Statement

Reference

aurA^Ubi-p63E.GFP, jub^E1 has abnormal mitotic cell cycle phenotype

(Sabino et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

jub^E1 has centrosome | somatic clone phenotype, enhanceable by aurA^Ubi-p63E.GFP

(Sabino et al., 2011)

jub^E1 has spindle | somatic clone phenotype, enhanceable by aurA^Ubi-p63E.GFP

(Sabino et al., 2011)

jub^E1 has neuroblast | somatic clone phenotype, enhanceable by aurA^Ubi-p63E.GFP

(Sabino et al., 2011)

Suppressed by

Statement

Reference

jub^E1 has centrosome | somatic clone phenotype, suppressible by aurA^87Ac-3/aur[+]

(Sabino et al., 2011)

jub^E1 has spindle | somatic clone phenotype, suppressible by aurA^87Ac-3/aur[+]

(Sabino et al., 2011)

jub^E1 has neuroblast | somatic clone phenotype, suppressible by aurA^87Ac-3/aur[+]

(Sabino et al., 2011)

Enhancer of

Statement

Reference

jub^E1 is an enhancer of wing phenotype of Rok^RNAi.UAS.cUa, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

Suppressor of

Statement

Reference

jub^E1 is a suppressor | partially of wing phenotype of Rok^CAT.UAS, Scer\GAL4^nub-AC-62

(Rauskolb et al., 2014)

NOT Suppressor of

Statement

Reference

jub^E1 is a non-suppressor of centrosome | increased number phenotype of SAK^Ubi.GFP

(Sabino et al., 2011)

jub^E1 is a non-suppressor of neuroblast phenotype of SAK^Ubi.GFP

(Sabino et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of jub^E1 enhances the reduction in wing size seen in flies expressing Rok^VDRC.cUa under the control of Scer\GAL4^nub-AC-62.

One copy of jub^E1 slightly suppresses the increase in wing size seen in flies expressing Rok^CAT.Scer\UAS under the control of Scer\GAL4^nub-AC-62.

(Rauskolb et al., 2014)

jub^E1 mutant neuroblasts expressing aur^{Ubi-p63E.T:Avic\GFP} exhibit mitotic spindle defects, with the proportion of disorganised spindles higher (61%) than that in jub^E1 or aur^{Ubi-p63E.T:Avic\GFP} mutants alone. Mitosis in these double mutants is highly perturbed, as revealed by an increased mitotic index. These cells show severe mitotic abnormalities, such as aneuploidy, polyploidy and anaphases with lagging chromosomes. These more severe defects are not present in jub^E1 mutants, where the mitotic index is not significantly different from that of wild-type.

The percentage of abnormal spindles in jub^E1 mutants is suppressed by removing one copy of aur because in jub^E1, aur^87Ac-3 double mutant cells the total number of spindle defects is decreased from 45% to 34%.

The generation of supernumerary centrosomes in SAK^{Ubi.T:Avic\GFP} mutant neuroblasts is not suppressed by a jub^E1 background.

(Sabino et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

jub^E1 is rescued by jub^GFP

(Sabino et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600160

w^* jub^E1 P{FRT(w^hs)}101/FM7a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

jub^E1

(Rauskolb et al., 2014, Sabino et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close