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General Information
Symbol
Dmel\mwh1
Species
D. melanogaster
Name
FlyBase ID
FBal0012675
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Caused by aberration
Cytology
Nature of the lesion
Statement
Reference

Rearrangement in which an inversion breaks the coding sequence after amino acid residue 367 (this breaks within the conserved FH3 domain).

The 3' end of the mwh gene could not be amplified from mwh1 mutants, suggesting that the mwh1 mutation may be associated with a deletion.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In mwh1 testes the number of normal individualization complexes (ICs), abnormal ICs and waste bags is similar to controls.

mwh1 homozygous adults show hair polarity defects in abdominal bristles.

mwh1 or mwh1/mwh6 flies show a 'multiple hair cell' phenotype in the wing.

Homozygous mwh1 mutant display wing hair defects.

Homozygotes have a multiple wing hair phenotype.

Hairs in the proximal-anterior part of the wing point towards the anterior margin and most cells form multiple hairs in mutant adults.

Some of the mwh1 mutant pupal wing cells fail to adopt a hexagonal shape.

mwh1 mutants display wing hair abnormalities.

mwh1 animals have a multiple wing hair phenotype; the mean number of hairs/cell at 25[o]C is 3.62.

mwh1/mwh6 animals have a multiple wing hair phenotype that is temperature sensitive; the mean number of hairs/cell is 1.83 at 21[o]C and 2.52 at 25[o]C.

Homozygotes show a multiple wing hair phenotype.

Prior to and during the appearance of prehair structures in the pupal wing, mutant cells show excess actin bundling across the apical surface of the cell, sometimes in a "starburst" pattern, with actin bundles radiating from the cell centre. Subsequently, prehair structures are seen at cell edges. The excess actin bundles often extend at least 1μm basally into the cytoplasm from the apical surface, particularly once prehair initiation is underway.

Mutants show no significant disruption of ovarian morphology.

Mutant clones in the wing show disruption of polarity, as indicated by wing hairs.

Hairs and denticles are smaller, more numerous and less orderly than those in wild type.

Wing cells of homozygous flies form more than one hair, with an average of almost four hairs per cell. Homozygous flies have disruptions in the pattern of wing hair polarity. The prehair initiation site is along the cell periphery, but not restricted to the distal vertex in pupal wing cells. Double mutants of mwh1 with fz1, dsh1, pk1, in1 or fy2 resemble mwh1 single mutants.

RK1 Hairs on the wings are grouped in numbers of 2-5 in the place of 1.

Affects the trichomes (hairs) of all body regions in the same general way: An increase in the number of elements is correlated with a reduction in length and a disturbance of orientation. Aristae and bracts are included in this pattern; bristles and other sensilla are not. Wing cells contain groups of 2-7 hairs instead of one hair per cell as in wild type; causes supernumerary trichomes over entire integument, but tufts of trichomes only in wing blade (Ouweneel, 1970); may be supernumerary hairs and sensilla on halteres (Ouweneel and van der Meer, 1973). Also causes disruption of polarity in legs, wings and halteres; may disrupt orientation of hairs on leg without affecting their numbers (Bryant and Schneiderman, 1969); trichomes on wings tend to diverge from vein L3 rather than parallel it as in wild type (Gubb and Garcia-Bellido, 1982). mwh/+ develop mwh phenotype following heat shock at or just prior to the time of cell-hair-extrusion (Mitchell and Petersen, 1984). Transplants of mutant wing discs to wild-type hosts develop autonomously (Ursprung and Hadorn, 1962). Widely used as a cell marker in the analysis of cuticular clones. The frequency of mwh spots after somatic recombination in mwh/+ flies increases with increase in the temperature at which the larvae and pupae are raised (Graf, 1986). RK1.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

mwh6/mwh1 has visible phenotype, enhanceable by Rho1unspecified/Rho1[+]

NOT Enhanced by
Statement
Reference

mwh1 has visible | recessive phenotype, non-enhanceable by Rab2351

Suppressed by
NOT suppressed by
Statement
Reference

mwh1 has visible | recessive phenotype, non-suppressible by Rab2351

Enhancer of
Statement
Reference

mwh[+]/mwh1 is an enhancer of visible phenotype of Scer\GAL4en-e16E, kermitGS2053

mwh[+]/mwh1 is an enhancer of visible | recessive phenotype of Rab2351

NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference

mwh1 has wing hair phenotype, non-enhanceable by Rab2351

mwh1 has wing hair | somatic clone phenotype, non-enhanceable by grhIM

mwh1 has wing hair | increased number | somatic clone phenotype, non-enhanceable by grhIM

Suppressed by
NOT suppressed by
Statement
Reference

mwh1 has wing hair phenotype, non-suppressible by Rab2351

mwh1 has wing hair | somatic clone phenotype, non-suppressible by grhIM

mwh1 has wing hair | increased number | somatic clone phenotype, non-suppressible by grhIM

Enhancer of
Statement
Reference

mwh[+]/mwh1 is an enhancer of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053

mwh[+]/mwh1 is an enhancer of wing hair phenotype of Rab2351

mwh[+]/mwh1 is an enhancer of wing hair & 1st posterior cell phenotype of inII53

mwh[+]/mwh1 is an enhancer of wing hair & 1st posterior cell phenotype of fy2

mwh[+]/mwh1 is an enhancer of wing hair & 1st posterior cell phenotype of in1

mwh1 is an enhancer of wing hair | somatic clone phenotype of ultA1

NOT Enhancer of
Statement
Reference

mwh1 is a non-enhancer of wing hair | somatic clone phenotype of grhIM

mwh1 is a non-enhancer of wing hair | increased number | somatic clone phenotype of grhIM

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

mwh1 is a non-suppressor of wing hair | somatic clone phenotype of grhIM

mwh1 is a non-suppressor of wing hair | increased number | somatic clone phenotype of grhIM

mwh1 is a non-suppressor of wing hair | somatic clone | cell autonomous phenotype of fz23

mwh1 is a non-suppressor of wing hair | somatic clone | cell non-autonomous phenotype of fz23

Additional Comments
Genetic Interactions
Statement
Reference

Heterozygosity significantly enhances the multiple hair cell phenotype of Scer\GAL4en.PU cmbScer\UAS.RA wings.

Homozygosity for cmbKO significantly suppresses the multiple hair cell phenotype of mwh1 and mwh1/mwh6 wings.

mwh1 dominantly enhances the trichome-phenotype of homozygous gishe01759 mutant clones in the wing.

Expression of gishGD10588 via Scer\GAL4en-e16E strongly enhances the wing hair phenotype associated with homozygous mwh1.

The wing hair phenotype of inIH56 mwh1 double mutants is indistinguishable from that of mwh1 single mutants.

Expression of in::mwhScer\UAS.cLa under the control of Scer\GAL4Act.PU almost completely rescues the wing hair defects of mwh1 mutants.

Expression of in::mwhScer\UAS.cLa under the control of Scer\GAL4ap-md544 strongly rescues the wing hair defects of inIH56 mwh1 double mutants on the dorsal surface of the wing.

The mwh1 single mutant wing hair phenotype is essentially identical to the mwh1 , Rab2351 double mutant phenotype.

mwh1 dominantly enhances the multiple wing hair phenotype of Rab2351 mutants.

Expression of Rho1Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ap-md544 partially but significantly suppresses the mwh1 multiple wing hair phenotype.

Expression of Rho1Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ap-md544 weakly suppresses the mwh1/mwh6 multiple wing hair phenotype.

Low levels of expression of Rho1V14.Scer\UAS under the control of Scer\GAL4ptc-559.1 at 25[o]C (the flies also carry Scer\GAL80ts.αTub84B) partially suppress the mwh1/mwh6 multiple wing hair phenotype.

Expression of RhoGEF2Scer\UAS.cUa under the control of Scer\GAL4ptc-559.1 partially suppresses the mwh1/mwh6 multiple wing hair phenotype.

The mwh1/mwh6 multiple wing hair phenotype is dominantly enhanced by Rho1unspecified.

Expression of RhoGAPp190Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1 enhances the mwh1/mwh6 multiple wing hair phenotype.

Expression of rokCAT.Scer\UAS under the control of Scer\GAL4ptc-559.1 partially suppresses the mwh1/mwh6 multiple wing hair phenotype.

Expression of rokCAT-KG.Scer\UAS under the control of Scer\GAL4ptc-559.1 does not suppress the mwh1/mwh6 multiple wing hair phenotype.

When grhIM mutant clones are made in an mwh1 mutant wing the grhIM phenotype is still seen. A slight additive effect is seen on the phenotype.

In somatic clones of cells that are homozygous for both mwh1 and ultA1, an increase is seen in the average number of wing hairs per cell.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (247)
Notes on Origin
Discoverer
Comments
Comments

Isolated from Moltrasio.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (32)