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General Information
Symbol
Dmel\gishGD10588
Species
D. melanogaster
Name
FlyBase ID
FBal0209638
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-gishIR
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  epilepsy
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Wings expressing gishGD10588 under the control of Scer\GAL4en-e16E display trichome defects.

Expression of gishGD10588 under the control of Scer\GAL4pnr-MD237 in the thorax induces most of the cells to project multiple trichomes in contrast to the single posterior-oriented trichomes seen in wild-type.

Clones of cells expressing gishGD10588 under the control of Scer\GAL4sev.PU induce abnormally rotated ommatidial clusters.

Expression of gishGD10588 under the control of Scer\GAL4dpp.blk1 results in multiple cellular hairs in the wing.

Expression of gishGD10588 under the control of Scer\GAL4en-e16E results in multiple cellular hairs in the wing and defects in ommatidial rotation.

Adults expressing gishGD10588 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

Adults expressing gishGD10588 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 20-30% of the Scer\GAL4pnr-MD237 expression domain.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4pnr-MD237 expression domain.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of gishGD10588 enhances the fused wing phenotype of flies expressing smoPKA12.Scer\UAS under the control of Scer\GAL4Bx-MS1096.

Expression of gishGD10588 under the control of Scer\GAL4elav-C155 partially suppresses the bang sensitive phenotype seen in parabss1/+ mutant females. Fewer flies exhibit bang-sensitivity and the threshold for seizure-like activity is partially rescued. gishGD10588 does not suppress the bang sensitive phenotypes in parabss1/parabss1 or parabss1/Y mutant flies.

Expression of gishGD10588 via Scer\GAL4en-e16E strongly enhances the wing hair phenotype associated with homozygous mwh1.

Co-expression of mwhScer\UAS.cYa with gishGD10588 under the control of Scer\GAL4en-e16E strongly suppresses the gishGD10588-induced wing hair phenotype.

Co-expression of Rab11Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP and gishGD10588 via Scer\GAL4en-e16E results in a strong enhancement of the wing hair phenotype compared with that resulting from the expression of either transgene alone.

Co-expression of gishGD10588 and Dcr-2Scer\UAS.cDa using Scer\GAL4hs.2sev results in planar cell polarity (PCP) and photoreceptor specification defects in ommatidia. The observed phenotypes include rare symmetrical ommatidia and R-cell loss.

Co-expression of gishGD10588 with stansev.PD under the control of Scer\GAL4hs.2sev enhances the ommatidial rotation defects induced by stansev.PD.

Co-expression of gishGD10588 with fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev enhances the ommatidial rotation defects induced by Scer\GAL4hs.2sev>fzScer\UAS.cAa.

nmoDB/+ enhances the ommatidial rotation defects induced by Scer\GAL4hs.2sev>gishGD10588.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Co-expression of gishScer\UAS.RB.T:Hsap\MYC with gishGD10588 under the control of Scer\GAL4en-e16E partially rescues the trichome phenotype.

Co-expression of gishD187N.Scer\UAS.T:Hsap\MYC with gishGD10588 under the control of Scer\GAL4en-e16E fails to rescue the trichome phenotype.

Co-expression of gishScer\UAS.RF.T:Hsap\MYC with gishGD10588 under the control of Scer\GAL4en-e16E fails to rescue the trichome phenotype.

An increase in apical cell area is observed in clones of Scer\GAL4Act5C.PI-driven gishGD10588-expressing cells in the pupal wing. Measurement of the apical area reveal a 60% increase relative to the adjacent wild-type cells, whereas analysis of the basolateral area does not show a significant increase. Transmission electron-microscopy reveal an increase in apical membrane projections in the mutant tissue compared with wild-type. Cells expressing gishGD10588 via Scer\GAL4Act5C.PI show no observable change in intensity or area of nuclear staining compared with wild-type cells.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
GishRNAi-3
gishGD10588
Name Synonyms
Secondary FlyBase IDs
    References (10)