Expression of gishGD10588 under the control of Scer\GAL4elav-C155 partially suppresses the bang sensitive phenotype seen in parabss1/+ mutant females. Fewer flies exhibit bang-sensitivity and the threshold for seizure-like activity is partially rescued. gishGD10588 does not suppress the bang sensitive phenotypes in parabss1/parabss1 or parabss1/Y mutant flies.
Co-expression of Rab11Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP and gishGD10588 via Scer\GAL4en-e16E results in a strong enhancement of the wing hair phenotype compared with that resulting from the expression of either transgene alone.
Co-expression of gishGD10588 and Dcr-2Scer\UAS.cDa using Scer\GAL4hs.2sev results in planar cell polarity (PCP) and photoreceptor specification defects in ommatidia. The observed phenotypes include rare symmetrical ommatidia and R-cell loss.
An increase in apical cell area is observed in clones of Scer\GAL4Act5C.PI-driven gishGD10588-expressing cells in the pupal wing. Measurement of the apical area reveal a 60% increase relative to the adjacent wild-type cells, whereas analysis of the basolateral area does not show a significant increase. Transmission electron-microscopy reveal an increase in apical membrane projections in the mutant tissue compared with wild-type. Cells expressing gishGD10588 via Scer\GAL4Act5C.PI show no observable change in intensity or area of nuclear staining compared with wild-type cells.