FlyBase Allele Report: Dmel\Strip[jam]

General Information

Symbol

Dmel\Strip^jam

Species

D. melanogaster

Name

jam packed

FlyBase ID

FBal0351806

Feature type

allele

Associated gene

Dmel\Strip

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(La Marca et al., 2019)

Progenitor genotype

Cytology

Description

C to T substitution at position 5530 of the Strip locus, resulting in a Gln codon being changed to a premature stop codon. The mutation is present in each predicted isoform of the gene; at position 2805 of isoform A, position 2370 of isoform B and position 2542 of isoform C.

(La Marca et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,323,342..3,323,342 [-]

Nucleotide change:

C3323342T

Reported nucleotide change:

C2805T

Amino acid change:

Q745term | Strip-PA; Q790term | Strip-PB; Q745term | Strip-PC

Reported amino acid change:

Q745term

Comment:

A nonsense mutation present in each predicted isoform of the Strip; at position 2805 of transcript isoform A, position 2370 of transcript isoform B and position 2542 of transcript isoform C.

(La Marca et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | third instar larval stage

(La Marca et al., 2019)

abnormal cell shape | third instar larval stage

(La Marca et al., 2019)

abnormal size | male | recessive | third instar larval stage

(La Marca et al., 2019)

abnormal size | male | third instar larval stage, with Df(3L)HR232

(La Marca et al., 2019)

abnormal size | male | third instar larval stage (with Df(3L)tc-1)

(La Marca et al., 2019)

abnormal size | male | third instar larval stage (with Strip^dogi)

(La Marca et al., 2019)

increased cell death | third instar larval stage

(La Marca et al., 2019)

increased occurrence of cell division | third instar larval stage

(La Marca et al., 2019)

lethal

(La Marca et al., 2019)

lethal, with Df(3L)HR232

(La Marca et al., 2019)

lethal (with Df(3L)1227)

(La Marca et al., 2019)

lethal (with Df(3L)ED208)

(La Marca et al., 2019)

viable (with Df(3L)BSC129)

(La Marca et al., 2019)

viable (with Df(3L)BSC368)

(La Marca et al., 2019)

viable (with Df(3L)ED4293)

(La Marca et al., 2019)

viable (with Df(3L)Exel6094)

(La Marca et al., 2019)

viable (with Df(3L)Exel6095)

(La Marca et al., 2019)

viable (with Df(3L)Exel6096)

(La Marca et al., 2019)

viable (with Df(3L)Exel6097)

(La Marca et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Strip^jam/Strip^jam, Strip^jam/Strip^dogi, Strip^jam/Df(3L)tc-1 and Strip^jam/Df(3L)HR232 mutants exhibit defective L3 male : small gonads with supernumerary strongly DAPI-positive cells throughout, whereas wild-type and Strip^jam/+ control gonads exhibit brighter DAPI-stained DNA in the apical region of the tissue, where the GSCs, gonialblasts, TA spermatogonia, CySCs and CyCs reside.

Further analysis of Strip^jam/Strip^jam L3 male gonads uncovers additional germline and somatic differentiation defects: the gonad is mainly occupied by small individualized germline cells containing small dot-like spectrosomes, rather than branched fusomes; stem cells (marked by Esg), spermatogonia (marked by Bam) are present throughout the gonad, instead of only being present at the tip/close to the tip of the gonad; early cyst cells can be found at the surface of the gonads, rather than interspersed between the germline cells; differentiated cysts and spermatocytes (marked by Topi) are observed only rarely; the germline lineage is less well enclosed by the somatic cell partners (marked by Arm); most somatic cells exhibit have disrupted morphology and disrupted nuclei morphology, and express markers for both early/stem cyst cell (Zfh1 or Tj) and differentiated cyst cell (Eya) fate. There are no obvious hub defects.

Individualized germline cells overproliferate (i.e. BrdU- or pH3-positive) and these dividing cells are present throughout the gonad, instead of only being present at the tip/close to the tip of the gonad. Apoptotic cells (i.e. TUNEL- or cleaved Caspase-3-positive) are also present throughout the gonad, while being negligible in controls.

(La Marca et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^K53R.UAS

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^GD10555

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/Tak1^KK108611

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, suppressible | partially by Traf6^GD7146/Scer\GAL4^C587

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, suppressible | partially by egr¹/egr¹

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, suppressible | partially by egr³/egr³

(La Marca et al., 2019)

NOT suppressed by

Statement

Reference

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/Rab5^{Q88L.UASp.YFP}

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/Mad^GD4121

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, non-suppressible by Stat92E^GD4492/Scer\GAL4^C587

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/btl::Egfr^λ.UAS

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/rl^Sem.S.UAS

(La Marca et al., 2019)

Strip^jam has abnormal size | recessive | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/imd^KK109863

(La Marca et al., 2019)

NOT Enhancer of

Statement

Reference

Strip[+]/Strip^jam is a non-enhancer of increased cell number | third instar larval stage | male phenotype of Egfr^DN.UAS, Scer\GAL4^C587

(La Marca et al., 2019)

NOT Suppressor of

Statement

Reference

Strip^jam/Strip^jam is a non-suppressor of decreased cell number | male | third instar larval stage phenotype of Scer\GAL4^C587, Stat92E^GD4492

(La Marca et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Strip^jam has gonad | male | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^K53R.UAS

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^GD10555

(La Marca et al., 2019)

Strip^jam has male germline cyst | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^GD10555

(La Marca et al., 2019)

Strip^jam has spermatogonium | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^GD10555

(La Marca et al., 2019)

Strip^jam has spermatocyte | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^GD10555

(La Marca et al., 2019)

Strip^jam has cyst cell of testis | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/bsk^GD10555

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^C587/Tak1^KK108611

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, suppressible | partially by Traf6^GD7146/Scer\GAL4^C587

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, suppressible | partially by egr¹/egr¹

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, suppressible | partially by egr³/egr³

(La Marca et al., 2019)

NOT suppressed by

Statement

Reference

Strip^jam has gonad | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/btl::Egfr^λ.UAS

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/rl^Sem.S.UAS

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/imd^KK109863

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/Rab5^{Q88L.UASp.YFP}

(La Marca et al., 2019)

Strip^jam has male germline stem cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/Rab5^{Q88L.UASp.YFP}

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, non-suppressible by Scer\GAL4^C587/Mad^GD4121

(La Marca et al., 2019)

Strip^jam has gonad | male | third instar larval stage phenotype, non-suppressible by Stat92E^GD4492/Scer\GAL4^C587

(La Marca et al., 2019)

NOT Enhancer of

Statement

Reference

Strip[+]/Strip^jam is a non-enhancer of gonad | third instar larval stage | male phenotype of Egfr^DN.UAS, Scer\GAL4^C587

(La Marca et al., 2019)

Strip[+]/Strip^jam is a non-enhancer of male germline stem cell | third instar larval stage phenotype of Egfr^DN.UAS, Scer\GAL4^C587

(La Marca et al., 2019)

Strip[+]/Strip^jam is a non-enhancer of cyst cell of testis | third instar larval stage phenotype of Egfr^DN.UAS, Scer\GAL4^C587

(La Marca et al., 2019)

NOT Suppressor of

Statement

Reference

Strip^jam/Strip^jam is a non-suppressor of gonad | male | third instar larval stage phenotype of Scer\GAL4^C587, Stat92E^GD4492

(La Marca et al., 2019)

Strip^jam/Strip^jam is a non-suppressor of male germline stem cell | third instar larval stage phenotype of Scer\GAL4^C587, Stat92E^GD4492

(La Marca et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Strip^jam is partially rescued by Scer\GAL4^C587/Strip^UAS.Venus

(La Marca et al., 2019)

Not rescued by

Strip^jam is not rescued by Scer\GAL4^nanos.PU/Strip^UAS.Venus

(La Marca et al., 2019)

Comments

Expressing Strip^UAS.Venus under the control of Scer\GAL4^C587 greatly rescues the L3 spermatogenesis defects of Strip^jam homozygotes but not their lethality.

(La Marca et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Strip^jam

(La Marca et al., 2019)

jam

(La Marca et al., 2019)

Name Synonyms

jam packed

(La Marca et al., 2019)

Secondary FlyBase IDs

References (1)

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