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General Information
Symbol
Dmel\bskGD10555
Species
D. melanogaster
Name
FlyBase ID
FBal0209184
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-bskRNAi, UAS-Bsk RNAi, bskRNAi, UAS-JNK-RNAi, bsk-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing bskGD10555 under the control of Scer\GAL4C587 (together with Dicer-2, for a more efficient RNAi) does not induce any obvious L3 spermatogenesis defects.

The expression of bskGD10555 under the control of Scer\GAL4GMR.long does not induce any eye phenotype (overall morphology, ommatidial array, interommatidial bristles), as compared to controls; eye discs do not show any apoptosis.

Expression of bskGD10555 under the control of Scer\GAL4Su(H).GBE (using tub-gal80[ts] to limit the time of expression) does not significantly affect cell proliferation in the adult gut.

6 days of expression of bskGD10555 in clones under the combined control of Scer\GAL4Act.PU and Gal80[ts] leads to a decrease in the proportion of mitotic cells in the adult midgut, as compared to control clones.

Expression of bskGD10555 in the posterior compartment of the imaginal wing disc, under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU does not affect levels of cell death.

Expression of bskGD10555 under the control of Scer\GAL4ple.PF does not lead to a significant difference in survival or climbing capability as compared to controls under normal conditions; however, these flies exhibit increased resistance to paraquat toxicity, with a significant increase in survival and climbing performance when exposed to paraquat, as compared to control flies exposed to the same treatment.

Transient expression of bskGD10555 in adults under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B strongly inhibits proliferation of intestinal stem cells compared with controls, and prevents tissue regeneration.

Transient expression of bskGD10555 in adults under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in significant lifespan shortening compared with wild-type.

Compared with controls, female flies expressing bskGD10555 under the control of Scer\GAL4bun-GSG5961 show a moderate, but significant, reduction in intestinal cell proliferation at old age. These flies are significantly longer lived when exposed to RU486 than isogenic siblings exposed to mock treatment.

Adults expressing bskGD10555 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of bskGD10555 using Scer\GAL4ey-OK107 results in axon defasciculation, axon degeneration and axon overextension phenotypes. These phenotypes are not as frequent if bskGD10555 expression is restricted to shorter periods of development (using the TARGET system).

Flies expressing bskGD10555 under the control of Scer\GAL4da.G32 more sensitive to dry starvation than wild-type controls.

Flies expressing bskGD10555 under the control of Scer\GAL4Ilp2.PR show a significantly reduced repression of growth (assayed by measuring wing area) when shifted from 25[o]C to 29[o]C compared to wild-type flies.

Flies expressing bskGD10555 under the control of Scer\GAL4Ilp2.PR are significantly more sensitive to paraquat than control flies.

Flies expressing bskGD10555 under the control of Scer\GAL4Ilp2.Switch.PK show increased sensitivity to paraquat compared to controls in the presence of RU486.

Expression under the control of Scer\GAL4pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 40-50% or 50-60% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in mild or strong thorax closure defects, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in an enlarged notum.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing either Ect4EP3610 or Myd88Scer\UAS.cMa under the control of Scer\GAL4elav.PU can be suppressed by co-expression of bskGD10555.

The larval brain overgrowth exhibited upon expression of bratHMS01121 under the control of Scer\GAL4da.PU is suppressed when bskGD10555 is co-expressed. The proportion of defective anaphases seen in larval brains expressing bratHMS01121 under the control of Scer\GAL4da.PU is significantly elevated upon co-expression of bskGD10555, but the level of cell death in these brains does not significantly change. The tumor growth rate of larval brain explants expressing bratHMS01121 under the control of Scer\GAL4da.PU is not suppressed upon co-expression bskGD10555.

The small wing size induced by Scer\GAL4C5-controlled expression of Dad1GD1006 is partially suppressed by the co-expression of bskGD10555. Co-expression of bskGD10555 suppresses the apoptosis, but not the compensatory cell division, induced by Scer\GAL4en.PU-controlled expression of Dad1GD1006 in third instar larval wing discs.

Visual system knockdown of bsk, through expression of bskGD10555 under the control of Scer\GAL4ey.PH in a sicilyE or MarfB mutant background significantly reduces lipid droplet accumulation.

The expression of vtdGL00522 under the control of Scer\GAL4en.PU (together with Dicer-2, for efficient RNAi) leads to a significant increase in apoptosis in the posterior compartment of the third instar larval wing disc, which is greatly suppressed by co-expressing bskGD10555.

Expression of vtdGL00522 under the control of Scer\GAL4en.PU (together with Dicer-2, for efficient RNAi) results in increased numbers of hemocytes on the surface of third instar larval wing discs, as compared to controls, which are suppressed by co-expressing bskGD10555.

Expression of vtdGL00522 under the control of Scer\GAL4en.PU (together with Dicer-2, for efficient RNAi) increases the level of reactive oxygen species in the posterior compartment of the third instar larval wing disc, as compared to controls, which are not decreased by co-expressing bskGD10555.

The increased cell proliferation in the adult gut characteristic for flies expressing Xbp1GD4745 under the control of Scer\GAL4Su(H).GBE (using tub-Gal80[ts] to limit the time of expression) is suppressed by co-expression of bskGD10555.

There is a significant reduction in chromosome instability tolerance induced by mad2GD16427 expression, when bskGD10555 is expressed in imaginal wing discs under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU. This leads to an increase in cell death.

Knockdown of Dark, through expression of DarkVDRC.cUa results in significantly reduced levels of cell death in imaginal wing disc cells expressing bskGD10555 and mad2GD16427.

The massive apoptosis seen in wing discs co-expressing p53Ct.GUS and mrnGD4089 under the control of Scer\GAL4Bx-MS1096 is practically abolished by co-expression of bskGD10555.

Co-expression of either one copy of kayFbz.Scer\UAS or two copies of JraJbz.Scer\UAS strongly enhances the axonal defects seen in Scer\GAL4ey-OK107, bskGD10555 animals.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
bskGD10555
Name Synonyms
Secondary FlyBase IDs
    References (31)