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General Information
Symbol
Dmel\Ire1UAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0356307
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a Ire1 cDNA. The coding sequence is tagged with Tag:V5.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Ire1UAS.Tag:V5 under the control of Scer\GAL4GMR.long leads to large anomalies to the external eyes; scanning electron microscopy analysis reveals a glassy eye surface characterized by ommatidial disruption, with severe loss of photoreceptors, and loss of interommatidial bristles; transmission electron microscopy analysis reveals a severe increase in the number of autophagosomes per ommatidium encircling mitochondria compared to controls, as well as amorphous structures which appear to be the partially degraded cellular debris.

In the eye disc, there is elevated apoptosis and increased numbers of autophagosomes/autolysosomes and decreased autophagic flux, as compared to controls.

Adults expressing Ire1UAS.Tag:V5 under the control of Scer\GAL4Ddc.PU show age-dependent impairment in climbing ability, associated with a progressive decrease in PPM1/2 and PPM3 neurons, despite of no significant changes in other DA neurons (PAL, PPL1 and PPL2 clusters) as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Ire1UAS.Tag:V5
Name Synonyms
Secondary FlyBase IDs
    References (1)