FlyBase Allele Report: Dmel\Atg17[KK101847]

General Information

Symbol

Dmel\Atg17^KK101847

Species

D. melanogaster

Name

FlyBase ID

FBal0231516

Feature type

allele

Associated gene

Dmel\Atg17

Associated Insertion(s)

Carried in Construct

P{KK101847}

Also Known As

Atg17 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101847}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101847

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell size, with Scer\GAL4^NP0001

(Wen et al., 2017)

short lived, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

Phenotype Manifest In

adult fat body | nutrition conditional, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

adult midgut epithelium, with Scer\GAL4^NP0001

(Wen et al., 2017)

adult midgut epithelium, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

autophagosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

lysosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

Detailed Description

Statement

Reference

Adulthood-only expression of Atg17^KK101847 under the combined control of Scer\GAL4^tub.PU and Gal80[ts] leads to intestinal barrier disfunction (as shown by the abnormal permeability to a non-absorbable food dye), leads to the failure to induce autophagy (assessed by the lysosome and autophagosome marker, Lysotracker) in the adult fat body in response to starvation, and leads to a decreased lifespan, as compared to controls. Adulthood-only expression under the combined control of Scer\GAL4^{Myo31DF-NP0001} and Gal80[ts] leads to pronounced defects in the midgut, namely a severe increase in cell size and abnormal epithelium morphology, as compared to controls.

(Wen et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Atg17^KK101847, Myc^UAS.cZa, Scer\GAL4^unspecified has increased cell growth rate | somatic clone | third instar larval stage phenotype, non-suppressible by cnc^EY08884, Scer\GAL4^unspecified