FlyBase Allele Report: Dmel\Atg1[GD7149]

Adulthood-only expression of Atg1^GD7149 under the control of Scer\GAL4^esg.PU leads to a severe increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

(Zhang et al., 2019)

The expression of Atg1^GD7149, driven by Scer\GAL4^NP0001 or driven by Scer\GAL4^hs.PB and induced by 1h heat-shocka during the 2 days before the experiment, but not when driven by Scer\GAL4^ppl.PU, induces a significantly higher mortality to Ecc15 enteric infection, as compared to controls; the Scer\GAL4^NP0001-driven expression also leads to a severe decrease in lipid droplet-containing autophagosomes (dual LS2- and Atg8-positive puncta) in the adult midgut under control conditions, as compared to controls; the Scer\GAL4^hs.PB-driven expression also abolishes the Ecc15 infection-induced lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut observed in controls.

(Lee et al., 2018)

Adults expressing Atg1^GD7149 under the control of Scer\GAL4^elav.PLu do not exhibit climbing defects compared to controls.

(Webber et al., 2018)

Starved third instar larvae expressing Atg1^GD7149 under the control of Scer\GAL4^Act5C.PI display a marked reduction in the area occupied by LysoTracker puncta in fat body cells compared to controls, furthermore the nucleation of Atg8 is abolished in these cells.

(Melani et al., 2017)

The clonal expression of Atg1^GD7149 under the control of Scer\GAL4^Act.PU results in a significant increase in the transverse cell area and a significant increase in relative mitochondrial content of midgut enterocytes during pupariation, as compared to controls.

(Liu et al., 2016)

Expression of Atg1^GD7149 under the control of Scer\GAL4^en-e16E does not induce either oxidative stress (monitored by levels of ROS) or cell death (visualized using [*]Casp3) in third instar larval wing discs.

(Liu et al., 2016)

Expression of Atg1^GD7149 under the control of Scer\GAL4^GMR.PF results in an eye indistinguishable from wild-type.

(Nandi et al., 2014)

Flies expressing Atg1^GD7149 under the control of Scer\GAL4^GMR.PU have a normal eye phenotype.

(Chen et al., 2012)

Adults expressing Atg1^GD7149 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Animals expressing Atg1^GD7149 under the control of Scer\GAL4^NP1 and analysed at +4 hours relative to puparium formation show a significant delay in midgut histolysis compared to controls. At +12 hours relative to puparium formation, larval midgut condensation is dramatically delayed in the mutants compared to controls.

(Denton et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7149

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg1^GD7149, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Ras85D^JF02478, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Atg1^GD7149, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible | partially by Rab11^JF02812, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Atg1^GD7149, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Egfr^JF01368, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Atg1^GD7149, Scer\GAL4^hs.PB has increased mortality | adult stage | conditional phenotype, suppressible by Acc^KK102082, Scer\GAL4^hs.PB

(Lee et al., 2018)

Atg1^GD7149, Scer\GAL4^hs.PB has abnormal immune response | adult stage phenotype, suppressible by Acc^KK102082, Scer\GAL4^hs.PB

(Lee et al., 2018)

Atg1^GD7149, Scer\GAL4^hs.PB has increased mortality | adult stage | conditional phenotype, suppressible by bmm^UAS.cUa, Scer\GAL4^hs.PB

(Lee et al., 2018)

Atg1^GD7149, Scer\GAL4^hs.PB has abnormal immune response | adult stage phenotype, suppressible by bmm^UAS.cUa, Scer\GAL4^hs.PB

(Lee et al., 2018)

Atg1^GD7149, Scer\GAL4^hs.PB has increased mortality | adult stage | conditional phenotype, suppressible by G6pd^UAS.cLa, Scer\GAL4^hs.PB

(Lee et al., 2018)

Atg1^GD7149, Scer\GAL4^hs.PB has abnormal immune response | adult stage phenotype, suppressible by G6pd^UAS.cLa, Scer\GAL4^hs.PB

(Lee et al., 2018)

Enhancer of

Statement

Reference

Atg1^GD7149, Scer\GAL4^elav.PLu is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4^elav.PLu, Sgsh^GD1578

(Webber et al., 2018)

Atg1^GD7149, Scer\GAL4^en-e16E is an enhancer of increased cell death | third instar larval stage phenotype of Scer\GAL4^en-e16E, vtd^GL00522

(Liu et al., 2016)

Atg1^GD7149, Scer\GAL4^Tub.PU is an enhancer of visible phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Sen et al., 2015)

Atg1^GD7149, Scer\GAL4^Mhc.PW is an enhancer of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Sen et al., 2015)

Atg1^GD7149, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Suppressor of

Statement

Reference

Atg1^GD7149, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PF, dor^NIG.3093R

(Nicolson et al., 2024)

Atg1^GD7149, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PF, lt^RNAi.UAS.cUa

(Nicolson et al., 2024)

Atg1^GD7149, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PF, Vps16A^HMS01716

(Nicolson et al., 2024)

Atg1^GD7149, Scer\GAL4^en-e16E is a suppressor | partially of increased mortality during development phenotype of Scer\GAL4^en-e16E, yki^HMS00041

(Tyra et al., 2020)

Atg1^GD7149, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size | third instar larval stage phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg1^GD7149, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg1^GD7149, Scer\GAL4^ptc-559.1 is a suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg1^GD7149, Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Other

Statement

Reference

Atg1^GD7149, Dbct^Δ, Scer\GAL4^elav.PLu has partially lethal - majority die phenotype

(Wu et al., 2025)

Atg1^GD7149, Dbct^Δ, Scer\GAL4^elav.PLu has some die during P-stage phenotype

(Wu et al., 2025)

Atg1^GD7149, Dbct^Δ, Scer\GAL4^elav.PLu has some die during larval stage phenotype

(Wu et al., 2025)

Atg1^GD7149, Dbct^Δ, Scer\GAL4^elav.PLu has decreased rate of larval locomotory behavior phenotype

(Wu et al., 2025)

Atg1^GD7149, Scer\GAL4^en-e16E, mad2^GD16427 has increased cell death | third instar larval stage phenotype

(Liu et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Atg1^GD7149, Scer\GAL4^NP0001 has embryonic/larval midgut | P-stage phenotype, non-enhanceable by Decay^GD8484, Scer\GAL4^NP0001

(Denton et al., 2009)

Suppressed by

Statement

Reference

Atg1^GD7149, Scer\GAL4^esg.PU has adult midgut epithelium phenotype, suppressible | partially by Rab11^JF02812, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Atg1^GD7149, Scer\GAL4^esg.PU has adult midgut epithelium phenotype, suppressible by Egfr^JF01368, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Atg1^GD7149, Scer\GAL4^esg.PU has adult midgut epithelium phenotype, suppressible by Ras85D^JF02478, Scer\GAL4^esg.PU

(Zhang et al., 2019)

Enhancer of

Statement

Reference

Atg1^GD7149, Scer\GAL4^en-e16E is an enhancer of wing disc | third instar larval stage phenotype of Scer\GAL4^en-e16E, vtd^GL00522

(Liu et al., 2016)

Atg1^GD7149, Scer\GAL4^Tub.PU is an enhancer of wing phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Sen et al., 2015)

Atg1^GD7149, Scer\GAL4^Mhc.PW is an enhancer of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Sen et al., 2015)

Atg1^GD7149, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Suppressor of

Statement

Reference

Atg1^GD7149, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Acn^UAS.cNa, Scer\GAL4^GMR.PF

(Nandi et al., 2014)

Atg1^GD7149, Scer\GAL4^ptc-559.1 is a suppressor of wing disc | third instar larval stage phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg1^GD7149, Scer\GAL4^ptc-559.1 is a suppressor of first posterior wing cell phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg1^GD7149, Scer\GAL4^ptc-559.1 is a suppressor of first basal wing cell phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg1^GD7149, Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Other

Statement

Reference

Atg1^GD7149, Scer\GAL4^en-e16E, mad2^GD16427 has wing disc | third instar larval stage phenotype

(Liu et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The dramatic increase in apoptosis (visualized by [*]Casp3 or acridine orange staining) observed in larval wing discs upon expression of vtd^GL00522 driven by Scer\GAL4^en-e16E is increased further by co-expression of Atg1^GD7149.

Co-expression of mad2^GD16427 with Atg1^GD7149 under the control of Scer\GAL4^en-e16E results in a dramatic increase in cell death (visualized by [*]Casp3 staining) in third instar larval wing discs.

(Liu et al., 2016)

Co-expression of Atg1^GD7149 increases the percentage of abnormal wing posture seen in flies with Pink1^{dsRNA.Scer\UAS} driven by Scer\GAL4^Mhc.PW or flies with park^KK107919 driven by Scer\GAL4^tub.PU.

(Sen et al., 2015)

Knockdown of Atg1 through expression of Atg1^GD7149 under the control of Scer\GAL4^GMR.PF suppresses the Acn^Scer\UAS.cNa-induced rough eye phenotype.

(Nandi et al., 2014)

The delayed midgut histolysis phenotype seen in animals expressing Atg1^GD7149 under the control of Scer\GAL4^NP1 after puparium formation is not enhanced by co-expression of decay^GD8484.

(Denton et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v16133

w¹¹¹⁸; P{GD7149}v16133

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7149

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Atg1^GD7149

Atg1^RNAi-GD

(Sênos Demarco et al., 2019)

Atg1[i, GD16133]

(Nagy et al., 2013)

atg-1^RNAi

(Scholz et al., 2019)

atg1^IR16133

(Mukherjee et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (51)

Report Sections

Open Close