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General Information
Symbol
Dmel\Atg1GD7149
Species
D. melanogaster
Name
FlyBase ID
FBal0198782
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ATG1-RNAi, UAS-atg1RNAi, UAS-Atg1 RNAi, Atg1RNAi, Atg1-RNAi, atg1 RNAi, UAS-Atg1IR, UAS-Atg1GD16133
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-only expression of Atg1GD7149 under the control of Scer\GAL4esg.PU leads to a severe increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

The expression of Atg1GD7149, driven by Scer\GAL4NP0001 or driven by Scer\GAL4hs.PB and induced by 1h heat-shocka during the 2 days before the experiment, but not when driven by Scer\GAL4ppl.PU, induces a significantly higher mortality to Ecc15 enteric infection, as compared to controls; the Scer\GAL4NP0001-driven expression also leads to a severe decrease in lipid droplet-containing autophagosomes (dual LS2- and Atg8-positive puncta) in the adult midgut under control conditions, as compared to controls; the Scer\GAL4hs.PB-driven expression also abolishes the Ecc15 infection-induced lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut observed in controls.

Adults expressing Atg1GD7149 under the control of Scer\GAL4elav.PLu do not exhibit climbing defects compared to controls.

Starved third instar larvae expressing Atg1GD7149 under the control of Scer\GAL4Act5C.PI display a marked reduction in the area occupied by LysoTracker puncta in fat body cells compared to controls, furthermore the nucleation of Atg8 is abolished in these cells.

The clonal expression of Atg1GD7149 under the control of Scer\GAL4Act.PU results in a significant increase in the transverse cell area and a significant increase in relative mitochondrial content of midgut enterocytes during pupariation, as compared to controls.

Expression of Atg1GD7149 under the control of Scer\GAL4en-e16E does not induce either oxidative stress (monitored by levels of ROS) or cell death (visualized using [*]Casp3) in third instar larval wing discs.

Expression of Atg1GD7149 under the control of Scer\GAL4GMR.PF results in an eye indistinguishable from wild-type.

Flies expressing Atg1GD7149 under the control of Scer\GAL4GMR.PU have a normal eye phenotype.

Adults expressing Atg1GD7149 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

Animals expressing Atg1GD7149 under the control of Scer\GAL4NP1 and analysed at +4 hours relative to puparium formation show a significant delay in midgut histolysis compared to controls. At +12 hours relative to puparium formation, larval midgut condensation is dramatically delayed in the mutants compared to controls.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The dramatic increase in apoptosis (visualized by [*]Casp3 or acridine orange staining) observed in larval wing discs upon expression of vtdGL00522 driven by Scer\GAL4en-e16E is increased further by co-expression of Atg1GD7149.

Co-expression of mad2GD16427 with Atg1GD7149 under the control of Scer\GAL4en-e16E results in a dramatic increase in cell death (visualized by [*]Casp3 staining) in third instar larval wing discs.

Co-expression of Atg1GD7149 increases the percentage of abnormal wing posture seen in flies with Pink1dsRNA.Scer\UAS driven by Scer\GAL4Mhc.PW or flies with parkKK107919 driven by Scer\GAL4tub.PU.

Knockdown of Atg1 through expression of Atg1GD7149 under the control of Scer\GAL4GMR.PF suppresses the AcnScer\UAS.cNa-induced rough eye phenotype.

The delayed midgut histolysis phenotype seen in animals expressing Atg1GD7149 under the control of Scer\GAL4NP1 after puparium formation is not enhanced by co-expression of decayGD8484.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Atg1GD7149
Atg1[i, GD16133]
Name Synonyms
Secondary FlyBase IDs
    References (30)