FlyBase Allele Report: Dmel\Atg7[GD11671]

General Information

Symbol

Dmel\Atg7^GD11671

Species

D. melanogaster

Name

FlyBase ID

FBal0198788

Feature type

allele

Associated gene

Dmel\Atg7

Associated Insertion(s)

Carried in Construct

P{GD11671}

Also Known As

Atg7 RNAi, UAS-atg7-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11671}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11671

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates myotonic dystrophy type 1

modeled by Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Tsou et al., 2015)

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^A30P.UAS

(Yan et al., 2019)

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

exacerbates carcinoma

modeled by Delta^UAS.cDa, lola^GS88A8

(Pérez et al., 2015)

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased size | adult stage, with Scer\GAL4^en-e16E

(Tyra et al., 2020)

short lived, with Scer\GAL4^hs.PU

(Chen et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Chen et al., 2012)

visible | adult stage, with Scer\GAL4^en-e16E

(Tyra et al., 2020)

Phenotype Manifest In

autophagosome, with Scer\GAL4^unspecified

(Chen et al., 2012)

eye, with Scer\GAL4^GMR.PU

(Chen et al., 2012)

ommatidium, with Scer\GAL4^GMR.PU

(Chen et al., 2012)

wing, with Scer\GAL4^en-e16E

(Tyra et al., 2020)

Detailed Description

Statement

Reference

The expression of Atg7^GD11671 under the control of Scer\GAL4^ap-md544 does not affect the curvature of the wing.

(Shinoda et al., 2019)

Flies expressing Atg7^GD11671 under the control of Scer\GAL4^Mef2.PU have similar lifespan, locomotion and mitochondrial structure as wild-type controls.

(Xu et al., 2019)

The expression of Atg7^GD11671 under the control of Scer\GAL4^GMR.long does not induce any eye phenotype (overall morphology, ommatidial array, interommatidial bristles), as compared to controls; eye discs do not show any apoptosis.

Expressing Atg7^GD11671 under the control of Scer\GAL4^Ddc.PU does not affect lifespan, adult climbing ability or number of DA neurons.

(Yan et al., 2019)

Expression of Atg7^GD11671 under the control of Scer\GAL4^GMR.PF results in an eye indistinguishable from wild-type.

(Nandi et al., 2014)

Expression of Atg7^GD11671 under the control of Scer\GAL4^hs.PU results in short lived adults compared to controls

(Chen et al., 2012)

Adults expressing Atg7^GD11671 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11671

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Atg7^GD11671, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Hsp27^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Chen et al., 2012)

Enhancer of

Statement

Reference

Atg7^GD11671, Scer\GAL4^GMR.long is an enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Atg7^GD11671, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

NOT Enhancer of

Statement

Reference

Atg7^GD11671, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{63Q.UAS.Tag:HA}

(Chen et al., 2012)

Suppressor of

Statement

Reference

Atg7^GD11671, Scer\GAL4^ap-md544 is a suppressor | partially of visible | adult stage phenotype of Decay^KK105137, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Atg7^GD11671, Scer\GAL4^Ddc.PU is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^Ddc.PU

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of increased cell death | third instar larval stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of decreased cell number | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^Ddc.PU is a suppressor of short lived phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^Ddc.PU

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^Ddc.PU is a suppressor of decreased cell number | adult stage | progressive phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^Ddc.PU

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^hs.PU is a suppressor | heat sensitive | partially of abnormal size | adult stage | heat sensitive phenotype of Scer\GAL4^hs.PU, Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

NOT Suppressor of

Statement

Reference

Atg7^GD11671, Scer\GAL4^ap-md544 is a non-suppressor of visible | adult stage phenotype of Dcp-1^KK106134, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Atg7^GD11671, Scer\GAL4^hs.PU is a non-suppressor of increased cell death | adult stage | heat sensitive phenotype of Scer\GAL4^hs.PU, Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

Other

Statement

Reference

Atg7^GD11671, Hsp27^UAS.Tag:HA, Scer\GAL4^Appl.PU has short lived phenotype

(Chen et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Atg7^GD11671, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Hsp27^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Chen et al., 2012)

Atg7^GD11671, Scer\GAL4^GMR.PU has ommatidium phenotype, non-suppressible by Hsp27^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Chen et al., 2012)

Enhancer of

Statement

Reference

Atg7^GD11671, Scer\GAL4^GMR.long is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Atg7^GD11671, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

NOT Enhancer of

Statement

Reference

Atg7^GD11671, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{63Q.UAS.Tag:HA}

(Chen et al., 2012)

Suppressor of

Statement

Reference

Atg7^GD11671, Scer\GAL4^ap-md544 is a suppressor | partially of wing phenotype of Decay^KK105137, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Atg7^GD11671, Scer\GAL4^Ddc.PU is a suppressor of dopaminergic PPM3 neuron | progressive phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^Ddc.PU

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of interommatidial bristle phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of ommatidium phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of eye disc phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^GMR.long is a suppressor of eye photoreceptor cell phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg7^GD11671, Scer\GAL4^hs.PU is a suppressor | heat sensitive | partially of adult somatic muscle cell of thorax | heat sensitive phenotype of Scer\GAL4^hs.PU, Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

Atg7^GD11671, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Acn^UAS.cNa, Scer\GAL4^GMR.PF

(Nandi et al., 2014)

NOT Suppressor of

Statement

Reference

Atg7^GD11671, Scer\GAL4^ap-md544 is a non-suppressor of wing phenotype of Dcp-1^KK106134, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Knockdown of Atg7 through expression of Atg7^GD11671 under the control of Scer\GAL4^GMR.PF suppresses the Acn^Scer\UAS.cNa-induced rough eye phenotype.

(Nandi et al., 2014)

Co-expression of Hsp27^{Scer\UAS.T:Ivir\HA1} does not rescue the rough eye phenotype caused by expression of Atg7^GD11671 under the control of Scer\GAL4^GMR.PU.

(Chen et al., 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of Atg7^GD11671 partially suppresses the decreased muscle area, but not the increased apoptosis seen in flies expressing Zzzz\CTG^{i480.Scer\UAS.cGa} under the control of Scer\GAL4^hs.PU in response to heat shock.

(Bargiela et al., 2015)

Co-expression of Atg7^GD11671 does not suppress the protective role of expression of Hsap\ATXN3^{Scer\UAS.WT.T:Zzzz\His6} in suppression of eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies. Co-expression of Atg7^GD11671 enhances eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies.

(Tsou et al., 2015)

The mild mosaic eye pigmentation defect caused by expression of Zzzz\CAG^{41Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU is enhanced by co-expression of Atg7^GD11671. A rough eye phenotype is also seen.

The rough eye defect caused by expression of Zzzz\CAG^{63Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU is not enhanced by co-expression of Atg7^GD11671.

(Chen et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

VDRC

v27432

w¹¹¹⁸ P{GD11671}v27432

v45558

w¹¹¹⁸; P{GD11671}v45558