FlyBase Allele Report: Dmel\Atg12[JF02704]

General Information

Symbol

Dmel\Atg12^JF02704

Species

D. melanogaster

Name

FlyBase ID

FBal0242291

Feature type

allele

Associated gene

Dmel\Atg12

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02704}

Also Known As

Atg12 RNAi, UAS-Atg12 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02704}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02704

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Tsou et al., 2015)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^695.UAS

(Zhuang et al., 2020)

ameliorates Parkinson's disease

modeled by Hsc70-5^KK100233

(Zhu et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cytoplasmic vesicle | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

fat cell | early third instar larval stage | somatic clone, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

Detailed Description

Statement

Reference

The expression of Atg12^JF02704 under the control of Scer\GAL4^Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls. Under standard conditions, there is no change in the number of autophagosome-like structures (Atg8a-positive), as compared to controls.

(Liu et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Atg12^JF02704, Scer\GAL4^GMR.long is an enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Atg12^JF02704, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Suppressor of

Statement

Reference

Atg12^JF02704, Scer\GAL4^nub-AC-62 is a suppressor of visible | adult stage phenotype of Scer\GAL4^nub-AC-62, synr^{UAS.ORF.GW.Tag:HA}

(Ikegawa et al., 2023)

Atg12^JF02704, Scer\GAL4^elav.PU is a suppressor of visible | adult stage phenotype of Hsc70-5^KK100233, Scer\GAL4^elav.PU

(Zhu et al., 2021)

Atg12^JF02704, Scer\GAL4^elav.PU is a suppressor of abnormal heat stress response | adult stage phenotype of Hsc70-5^KK100233, Scer\GAL4^elav.PU

(Zhu et al., 2021)

Atg12^JF02704, Scer\GAL4^elav.PU is a suppressor of paralytic | adult stage | heat sensitive phenotype of Hsc70-5^KK100233, Scer\GAL4^elav.PU

(Zhu et al., 2021)

Atg12^JF02704, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsc70-5^KK100233, Scer\GAL4^elav.PU

(Zhu et al., 2021)

Atg12^JF02704, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsc70-5^KK100233, Scer\GAL4^elav.PU

(Zhu et al., 2021)

NOT Suppressor of

Statement

Reference

Atg12^JF02704, Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg12^JF02704, Scer\GAL4^Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Phenotype Manifest In

Enhancer of

Statement

Reference

Atg12^JF02704, Scer\GAL4^GMR.long is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Atg12^JF02704, Scer\GAL4^bbg-C96 is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Suppressor of

Statement

Reference

Atg12^JF02704, Scer\GAL4^nub-AC-62 is a suppressor of wing phenotype of Scer\GAL4^nub-AC-62, synr^{UAS.ORF.GW.Tag:HA}

(Ikegawa et al., 2023)

Atg12^JF02704, Scer\GAL4^elav.PU is a suppressor of wing phenotype of Hsc70-5^KK100233, Scer\GAL4^elav.PU

(Zhu et al., 2021)

Atg12^JF02704, Scer\GAL4^Cg.PA is a suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Atg12^JF02704, Scer\GAL4^Cg.PA is a suppressor | partially of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

NOT Suppressor of

Statement

Reference

Atg12^JF02704, Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The wing nicking phenotype caused by expression of dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also co-expressing Atg12^JF02704.

(Kwon et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of Atg12^JF02704 does not suppress the protective role of expression of Hsap\ATXN3^{Scer\UAS.WT.T:Zzzz\His6} in suppression of eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies. Co-expression of Atg12^JF02704 enhances eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies.

(Tsou et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27552

y¹ v¹; P{TRiP.JF02704}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Atg12^JF02704

Atg12[JF02704]

(Nagy et al., 2015)

atg12<up>IR JF02704</up>

(Mukherjee et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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