FlyBase Allele Report: Hsap\APP[695.UAS]

General Information

Symbol

Hsap\APP^695.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0095694

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-APP.695}

Also Known As

UAS-APP, P{UAS:APP₆₉₅}, UAS-APP₆₉₅, APP₆₉₅, UAS-AβPP

Key Links

Transgenic product class

wild_type

(Fossgreen et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Fossgreen et al., 1998)

Mutagen

in vitro construct

(Fossgreen et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-APP.695}

Cytology

Description

Wild-type Hsap\APP695 is expressed under the control of UASt regulatory sequences.

(Fossgreen et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Xu et al., 2016, Peng et al., 2015, Copenhaver et al., 2011, Greeve et al., 2004)

CEA with Hsap\BACE1^UAS.cGa

(Zhang et al., 2022, Miguel et al., 2020, Mhatre et al., 2014, Mhatre et al., 2014, Rajendran et al., 2008)

CEA with Hsap\BACE1^UAS.cGa, Psn^+14.UAS

(Rajendran et al., 2008)

model of attention deficit hyperactivity disorder

CEA with Hsap\BACE1^UAS.cGa

(Zhang et al., 2015)

model of Alzheimer's disease 1

CEC

(Zhuang et al., 2020)

CEA

(Gunawardena et al., 2013)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease

is ameliorated by polo^GL00014

(Peng et al., 2015)

is ameliorated by polo^HMS00530

(Peng et al., 2015)

is ameliorated by polo¹

(Peng et al., 2015)

is ameliorated by Psn^B3

(Greeve et al., 2004)

is exacerbated by Psn^L235P.UAS

(Greeve et al., 2004)

is ameliorated by Rab5^{S43N.UASp.YFP}

(Xu et al., 2016)

model of Alzheimer's disease 1

is ameliorated by Atg12^JF02704

(Zhuang et al., 2020)

is ameliorated by Atg13^Δ81

(Zhuang et al., 2020)

is ameliorated by Atg7^JF02787

(Zhuang et al., 2020)

is ameliorated by STUB1^HMS00889

(Zhuang et al., 2020)

is ameliorated by STUB1^HMS00986

(Zhuang et al., 2020)

is ameliorated by STUB1^I

(Zhuang et al., 2020)

is ameliorated by Psn¹⁴³

(Gunawardena et al., 2013)

is ameliorated by Psn^I2

(Gunawardena et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

One copy of Df(2R)Egfr5 exacerbates the neurodegeneration seen in Alzheimer's disease modeled by expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa.

(Muhammad et al., 2008)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior, with Scer\GAL4^elav-C155

(Hu et al., 2014)

abnormal courtship behavior, with Scer\GAL4^NP0021

(Hu et al., 2014)

abnormal courtship behavior | male, with Scer\GAL4^NP0021

(Peng et al., 2015)

abnormal eye color, with Scer\GAL4^GMR.PU

(Peng et al., 2015)

abnormal locomotor behavior, with Scer\GAL4^Appl.G1a

(Wang et al., 2014)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^Appl.G1a

(Peng et al., 2015)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Appl.G1a

(Peng et al., 2015)

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^GMR.PU

(Peng et al., 2015)

abnormal neuroanatomy | progressive, with Scer\GAL4^GMR.PU

(Copenhaver et al., 2011)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Appl.G1a

(Xu et al., 2016, Peng et al., 2015)

increased cell death, with Scer\GAL4^ap-md544

(Wang et al., 2014)

increased cell death, with Scer\GAL4^Appl.G1a

(Wang et al., 2014)

increased cell death, with Scer\GAL4^elav-C155

(Wang et al., 2014)

increased cell death, with Scer\GAL4^GMR.PF

(Wang et al., 2014, Greeve et al., 2004)

increased cell death, with Scer\GAL4^pnr-MD237

(Wang et al., 2014)

increased cell death, with Scer\GAL4^ptc-559.1

(Wang et al., 2014)

increased cell death, with Scer\GAL4^sd-SG29.1

(Wang et al., 2014)

lethal, with Scer\GAL4^Act5C.PI

(Wang et al., 2010)

melanotic mass phenotype | adult stage, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

partially lethal - majority die, with Scer\GAL4^Act5C.PI

(Greeve et al., 2004)

partially lethal - majority die, with Scer\GAL4^Act.PU

(Copenhaver et al., 2011)

partially lethal - majority die | drug conditional, with Scer\GAL4^Act5C.PI

(Wang et al., 2010)

short lived, with Scer\GAL4^Appl.G1a

(Peng et al., 2015)

short lived, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

visible, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

visible, with Scer\GAL4^GMR.PU

(Peng et al., 2015)

visible, with Scer\GAL4^Mz1087.hx

(Fossgreen et al., 1998)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^ptc-559.1

(Wang et al., 2014)

axon | third instar larval stage, with Scer\GAL4^Appl.G1a

(Xu et al., 2016)

eye, with Scer\GAL4^GMR.PF

(Wang et al., 2014)

eye, with Scer\GAL4^GMR.PU

(Peng et al., 2015)

eye disc | third instar larval stage, with Scer\GAL4^GMR.PU

(Peng et al., 2015)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Greeve et al., 2004)

larval segmental nerve | third instar larval stage, with Scer\GAL4^Appl.G1a

(Xu et al., 2016)

melanotic mass | adult stage, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

NMJ bouton | third instar larval stage, with Scer\GAL4^Appl.G1a

(Peng et al., 2015)

retina | progressive, with Scer\GAL4^GMR.PU

(Copenhaver et al., 2011)

scutellum, with Scer\GAL4^pnr-MD237

(Wang et al., 2014)

type Ib terminal bouton | third instar larval stage, with Scer\GAL4^Appl.G1a

(Peng et al., 2015)

type Is terminal bouton | third instar larval stage, with Scer\GAL4^Appl.G1a

(Peng et al., 2015)

wing, with Scer\GAL4^Appl.G1a

(Peng et al., 2015)

wing, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

wing, with Scer\GAL4^Mz1087.hx

(Fossgreen et al., 1998)

wing, with Scer\GAL4^sd-SG29.1

(Wang et al., 2014)

wing pouch, with Scer\GAL4^sd-SG29.1

(Wang et al., 2014)

Detailed Description

Statement

Reference

Expression of Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Appl.G1a results in impaired axonal transport due to axonal blockages in the segmental nerves in third instar larvae.

(Xu et al., 2016)

Flies with Hsap\APP^695.Scer\UAS overexpressed by Scer\GAL4^Appl.G1a have a severe wing expansion defect and larvae have a significant increase in the number of boutons and branches at the larval neuromuscular junction (NMJ) compared to wild type. There is a significant decrease in the number of type Ib boutons and a significant increase in the number of type Is boutons at the NMJ of larvae with Hsap\APP^695.Scer\UAS overexpressed by Scer\GAL4^Appl.G1a. Larvae (significantly reduced crawling speed) and adults (significantly reduced climbing speed) with Hsap\APP^695.Scer\UAS overexpression by Scer\GAL4^Appl.G1a have locomotory defects. Flies with Hsap\APP^695.Scer\UAS overexpressed by Scer\GAL4^Appl.G1a have a severely shortened lifespan.

When flies with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a are reared at 17[o]C and only put at 25[o]C after eclosion, they do not display the wing expansion defect or locomotor defects (tested at 2 days old) that are seen when they are reared at 25[o]C. Climbing ability declines dramatically with aging in flies with adult-specific (reared at 17[o]C, 25[o]C after eclosion) expression of Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a. Lifespan is reduced in flies with adult-specific (reared at 17[o]C, 29[o]C after eclosion) expression of Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a.

Male flies with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^fru-NP0021 show a courtship defect (eliminates courtship preference for younger mates).

Flies with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^GMR.PU have small rough eyes with reduced pigmentation. Third instar larvae with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^GMR.PU show abnormal cell cycle re-entry at the eye disc.

(Peng et al., 2015)

3-day-old naive flies expressing Hsap\APP^695.Scer\UAS in the central nervous system under the control of Scer\GAL4^elav-C155 are unable to distinguish younger females from older females (as wild-type young males can), resembling the phenotype seen in 60-day-old males. In contrast, Hsap\APP^695.Scer\UAS-expressing males show no defects in courtship index compared with controls, indicating that Hsap\APP^695.Scer\UAS expression does not compromise males' overall courtship ability or courting interest in females. The preferential index (between young and old females) decreases significantly compared with that of controls, suggesting males' discriminating ability is abrogated by pan-neuronal expression of Hsap\APP^695.Scer\UAS.

Expression of Hsap\APP^695.Scer\UAS in the courtship circuit, under the control of Scer\GAL4^fru-NP0021 abolishes the male preference for younger mates but does not affect their total courtship index.

(Hu et al., 2014)

No body wall contraction defects are seen in third instar larvae expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^elav-C155. Crawling distance and rate are similar to controls.

(Mhatre et al., 2014)

Expression of Hsap\APP^695.Scer\UAS in the nervous system, under the control of the pan-neuronal Scer\GAL4^elav-C155 results in elevated cell death in the central nervous system.

Expression of Hsap\APP^695.Scer\UAS in the developing eye, under the control of Scer\GAL4^GMR.PF induces strong cell death in third-instar larval eye discs and produces rough eyes with reduced size in the adults.

Expression of Hsap\APP^695.Scer\UAS in the developing thorax, under the control of Scer\GAL4^pnr-MD237 or Scer\GAL4^ap-md544 triggers cell death in the thorax region of third-instar larval wing disc and produces a small scutellum phenotype in the adult thorax.

Expression of Hsap\APP^695.Scer\UAS along the anterior/posterior compartment boundary in the larval wing disc, under the control of Scer\GAL4^ptc-559.1, induces robust cell death and produces a loss of the anterior cross vein in the adult wing.

Expression of Hsap\APP^695.Scer\UAS in the developing wing pouch, driven by Scer\GAL4^sd-SG29.1 provokes extensive cell death and generates a small wing phenotype in the adults.

Expression of Hsap\APP^695.Scer\UAS in the central nervous system, under the control of Scer\GAL4^Appl.G1a, results in enhanced cell death and a considerable reduction in locomotory ability.

Expression of Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^sd-SG29.1 results in strong activation of W and reduced wing pouch size.

(Wang et al., 2014)

Expression of Hsap\APP^695.Scer\UAS driven pan-neuronally by Scer\GAL4^elav-C155 results in crumpled wings and presence of melanotic masses on the abdomen and proboscis; penetrance is around tenfold reduced compared to what is seen with expression of Hsap\BACE1^Scer\UAS.cGa. Compared to genetic controls, Scer\GAL4^elav-C155>Hsap\APP^695.Scer\UAS young adults have significantly reduced survival. Scer\GAL4^elav-C155>Hsap\APP^695.Scer\UAS fly brains have similar size mushroom bodies as controls (significantly larger calyx and lobes than is seen with expression of Hsap\BACE1^Scer\UAS.cGa). Flies (2-10 and 12-20 days old) with expression of Hsap\APP^695.Scer\UAS driven by Scer\GAL4^elav-C155 show similar climbing ability to controls.

(Chakraborty et al., 2011)

Expression of Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^GMR.PU results in accumulation of Aβ peptides, amyloid deposits and age-dependent retinal degeneration.

Flies expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Act.PU exhibit premature lethality. Dietary isradipine significantly increases the survival rate in a concentration-dependent manner. No effect is seen when the larvae are fed verapamil.

(Copenhaver et al., 2011)

Ubiquitous expression of Hsap\APP^695.Scer\UAS (under the control of Scer\GAL4^Act5C.PI) results in a survival rate of about 5% compared to control flies. Raising these flies on food containing increased concentration of HSB-13 results in a significant increase in the survival rate ranging from 65% at 50υM to 44% at 2.5υM compared with equally treated control flies. These survival rates are approximately 10 times higher compared with untreated flies.

(Wang et al., 2010)

Individuals simultaneously heterozygous for Df(2R)Egfr5 and expressing Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act.PU are short lived compared to controls.

(Muhammad et al., 2008)

Flies expressing Hsap\APP^695.Scer\UAS in the developing eye under the control of Scer\GAL4^GMR.PF leads to no detectable abnormalities.

(Li et al., 2007)

Expression of Hsap\APP^695.Scer\UAS in the eye under the control of Scer\GAL4^GMR.PF results in age-dependent photoreceptor degeneration. Amyloid plaques form in the retinae of these flies but this occurs after the onset of neurodegeneration.

(Greeve et al., 2004)

Flies expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Mz1087.hx have a wing blister phenotype and develop a deformation in one or both wings. Large blisters may occupy more than 50% of the wing surface and in extreme cases the wings appear like balloons. The blisters appear transparent or black, are filled with hemolymph and occur in 5-20% of flies at 25^oC. The pattern of wing veins is normal.

(Fossgreen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has visible | adult stage phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has abnormal neuroanatomy | adult stage phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Psn^L235P.UAS, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

Suppressed by

Statement

Reference

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Rab5^{S43N.UASp.YFP}, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by polo[+]/polo¹

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior | third instar larval stage phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior | third instar larval stage phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior | third instar larval stage phenotype, suppressible | partially by polo[+]/polo¹

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by polo[+]/polo¹

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has short lived phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has short lived phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has short lived phenotype, suppressible | partially by polo[+]/polo¹

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^NP0021 has abnormal courtship behavior | male phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^NP0021

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^NP0021 has abnormal courtship behavior | male phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^NP0021

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has abnormal eye color phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has abnormal eye color phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, suppressible by Psn^{527.D447A.GMR}, Scer\GAL4^ptc-559.1

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, suppressible by foxo^Δ94

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^pnr-MD237 has increased cell death phenotype, suppressible by foxo^Δ94

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, suppressible by foxo^KK108590, Scer\GAL4^ptc-559.1

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior phenotype, suppressible by foxo^Δ94

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal locomotor behavior phenotype, suppressible by foxo^KK108590, Scer\GAL4^Appl.G1a

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^sd-SG29.1 has increased cell death phenotype, suppressible by foxo^Δ94

(Wang et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype, suppressible | partially by Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype, suppressible | partially by Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has visible phenotype, suppressible by Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has visible phenotype, suppressible by Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by Psn^B3/Psn[+]

(Greeve et al., 2004)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has partially lethal - majority die phenotype, suppressible | partially by Psn^C4/Psn[+]

(Greeve et al., 2004)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible | partially by Hsap\BACE1^UAS.cGa, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

NOT suppressed by

Statement

Reference

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Rab5^UASp.YFP, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Enhancer of

Statement

Reference

Hsap\APP^695.UAS, Scer\GAL4^elav-C155 is an enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155

(Mhatre et al., 2014)

Other

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has decreased rate of movement | adult stage phenotype

(Zhang et al., 2015)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^{elav.Switch.PO} has increased rate of movement | adult stage | RU486 conditional phenotype

(Zhang et al., 2015)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^{elav.Switch.PO} has increased rate of movement | adult stage phenotype

(Zhang et al., 2015)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal neuroanatomy | third instar larval stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has visible | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal neuroanatomy | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has melanotic mass phenotype | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal memory | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal size | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage | progressive phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype

(Chakraborty et al., 2011, Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has visible phenotype

(Tsuda et al., 2010)

Df(2R)Egfr5/+, Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has short lived phenotype

(Muhammad et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has wing vein phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has brain | adult stage phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, enhanceable by Psn^L235P.UAS, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, enhanceable by Psn^+14.UAS, Scer\GAL4^Act5C.PI

(Greeve et al., 2004)

Suppressed by

Statement

Reference

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has larval segmental nerve | third instar larval stage phenotype, suppressible by Rab5^{S43N.UASp.YFP}, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has axon | third instar larval stage phenotype, suppressible by Rab5^{S43N.UASp.YFP}, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has wing phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has wing phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has wing phenotype, suppressible | partially by polo[+]/polo¹

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has NMJ bouton | third instar larval stage phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has NMJ bouton | third instar larval stage phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has NMJ bouton | third instar larval stage phenotype, suppressible | partially by polo[+]/polo¹

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has type Ib terminal bouton | third instar larval stage phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has type Is terminal bouton | third instar larval stage phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has type Ib terminal bouton | third instar larval stage phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has type Is terminal bouton | third instar larval stage phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has type Is terminal bouton | third instar larval stage phenotype, suppressible | partially by polo[+]/polo¹

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has eye disc | third instar larval stage phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PU has eye disc | third instar larval stage phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^ptc-559.1 has anterior crossvein phenotype, suppressible by Psn^{527.D447A.GMR}, Scer\GAL4^ptc-559.1

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^ptc-559.1 has anterior crossvein phenotype, suppressible by foxo^Δ94

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^pnr-MD237 has scutellum phenotype, suppressible by foxo^Δ94

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^ptc-559.1 has anterior crossvein phenotype, suppressible by foxo^KK108590, Scer\GAL4^ptc-559.1

(Wang et al., 2014)

Hsap\APP^695.UAS, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible by foxo^Δ94

(Wang et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, suppressible by Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, suppressible by Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible | partially by Hsap\BACE1^UAS.cGa, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

Hsap\APP^695.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible by Psn^B3/Psn[+]

(Greeve et al., 2004)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, suppressible | partially by Psn^C4/Psn[+]

(Greeve et al., 2004)

NOT suppressed by

Statement

Reference

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has larval segmental nerve | third instar larval stage phenotype, non-suppressible by Rab5^UASp.YFP, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has axon | third instar larval stage phenotype, non-suppressible by Rab5^UASp.YFP, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has larval segmental nerve | third instar larval stage phenotype, non-suppressible by Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a has axon | third instar larval stage phenotype, non-suppressible by Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Other

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has type I bouton phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has wing | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has mushroom body | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has proboscis | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype

(Tsuda et al., 2010, Greeve et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The presence of axonal blockages in the segmental nerves characteristic for third instar larvae expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Appl.G1a is rescued by co-expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP}, but not Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} or Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Xu et al., 2016)

Co-expression of polo^HMS00530 or polo^GL00014 partially suppresses the wing expansion defects, shortened lifespan, locomotion defects (reduced crawling speed in larvae or reduced climbing speed in adults), and increases of bouton and branch number (including decreases in Ib boutons and increases in Is boutons) seen at the larval neuromuscular junction in animals with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a.

Co-expression of polo^HMS00530 or polo^GL00014 partially suppresses the courtship defects in male flies with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^fru-NP0021.

Co-expression of polo^HMS00530 or polo^GL00014 partially suppresses the eye development defects in adults and the abnormal cell cycle re-entry at the third instar larval eye disc seen when Hsap\APP^695.Scer\UAS is driven by Scer\GAL4^GMR.PU.

Adult-specific (reared at 17[o]C, 25[o]C after eclosion) expression of polo^HMS00530 or polo^GL00014 partially suppresses the decline in climbing ability seen in older flies expressing Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a during adulthood. Adult-specific (reared at 17[o]C, 29[o]C after eclosion) expression of polo^HMS00530 or polo^GL00014 partially suppresses shortened lifespan in flies with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a during adulthood.

polo¹/+ partially suppresses the wing expansion defects, shorter lifespan, locomotion defects (larval and adult), and increases of Is bouton numbers (but does not suppress branch number changes or the decrease of Ib boutons) at the larval neuromuscular junction seen in animals with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a.

(Peng et al., 2015)

Co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa driven by Scer\GAL4^{elav.Switch.PO} driven by Scer\GAL4^{elav.Switch.PO} (with 200um RU486) results in significant hyperactivity (including nocturnal hyperactivity followed by a rapid decline, the effect is more prominent in male flies, and the effect disappears as flies age); this effect is more striking on a diet with a high carbohydrate to protein ratio (compared to low carbohydrate to protein ratio).

Co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa driven by Scer\GAL4^elav-C155 results in significant hypoactivity compared to controls, though total activity at night is not significantly altered (it is significantly decreased during the day).

(Zhang et al., 2015)

Larvae co-expressing Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 show a significant decrease in third instar larval body wall contractions.

The crawling defects seen when Hsap\BACE1^Scer\UAS.cGa is expressed under the control of Scer\GAL4^elav-C155 are enhanced upon co-expression of Hsap\APP^695.Scer\UAS.

Co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 results in structural changes in the synapse of the third instar larval muscle 6 and 7 neuromuscular junctions (NMJs). The overall number of boutons is reduced compared to controls, and this is due to a reduced number of type 1s boutons; the number of 1b boutons is similar to wild type. Both the number of motor neuron branches and the size of muscles 6 and 7 at the NMJ are similar to controls. The density of presynaptic release sites (brp-positive active zones) in motor neurons is also normal, but mitochondrial localisation defects are observed.

(Mhatre et al., 2014)

Expression of both Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 leads to a subset of flies showing melanotic masses on the ventral proboscis, curled wings, and these flies show a significant and progressive defect in climbing ability beginning at 42 days post-eclosion, a significant decrease in the size of the mushroom body, and a significant decrease in immediate recall memory in a conditioned courtship suppression assay, but do not show a significant decrease in lifespan nor any significant defect in learning ability as compared to controls.

(Mhatre et al., 2014)

Expression of Psn^{527.D447A.GMR} suppresses the Hsap\APP^695.Scer\UAS-induced cell death seen upon expression in the developing wing (under the control of Scer\GAL4^ptc-559.1).

A foxo^Δ94 mutant background suppresses the Scer\GAL4^ptc-559.1-->Hsap\APP^695.Scer\UAS induced cell death in the larval wing disc and the loss of anterior cross vein found in these mutants.

Knockdown of foxo through expression of foxo^KK108590 suppresses the Scer\GAL4^ptc-559.1-->Hsap\APP^695.Scer\UAS induced cell death in the larval wing disc and the loss of anterior cross vein found in these mutants.

Knockdown of foxo through expression of foxo^KK108590 significantly suppresses the locomotory defects found upon expression of Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Appl.G1a.

A foxo^Δ94 mutant background suppresses the induced scutellum phenotype found upon expression of Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^ap-md544 and the Scer\GAL4^sd-SG29.1-->Hsap\APP^695.Scer\UAS-induced small wing phenotype.

A foxo^Δ94 heterozygous background significantly suppresses the locomotory defects found upon expression of Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Appl.G1a.

A foxo^Δ94 heterozygous background considerably suppresses the reduced wing size (and strong W expression) found in flies expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^sd-SG29.1.

(Wang et al., 2014)

The pan-neuronal co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 leads to a decrease in adult lifespan, as compared to controls.

(Chakraborty et al., 2011)

Co-expression of Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^elav-C155 results in reduced lifespan compared with controls.

The reduced lifespan of flies co-expressing Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^elav-C155 is partially suppressed by the co-expression of Ide^Scer\UAS.cTa.

The ectopic wing vein phenotype induced by co-expressing Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Act5C.PI is suppressed by the co-expression of Ide^Scer\UAS.cTa.

(Tsuda et al., 2010)

One copy of Df(2R)Egfr5 enhances the wing vein phenotype seen when Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa are co-expressed under the control of Scer\GAL4^Act.PU. The neurodegeneration is also increased, with at least 50% of flies showing clear evidence of neurodegeneration throughout the central brain compared to ~10% in controls and, unlike in controls, a significant fraction show dramatic loss of brain tissue.

(Muhammad et al., 2008)

Coexpression of Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS, under the control of Scer\GAL4^GMR.PF, leads to a retinal degeneration phenotype that is less severe than that seen when Hsap\APP^695.Scer\UAS is expressed alone.

A Psn^B3/+ background rescues the age-dependent retinal neurodegeneration phenotype seen when Hsap\APP^695.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF. Further, coexpression of Psn^{L235P.Scer\UAS} with Hsap\APP^695.Scer\UAS, under the control of Scer\GAL4^GMR.PF, enhances the degeneration.

Flies that coexpress Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI exhibit ectopic wing veins. This phenotype is enhanced in flies that express Psn^+14.Scer\UAS in addition to Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI.

A Psn^C4/+ background partially rescues the ectopic wing vein formation and semi-lethality of flies that coexpress Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI.

(Greeve et al., 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into SL-2 cells with a Scer\GAL4 expression plasmid. The expression and metabolism of Hsap\APP^695.Scer\UAS protein in these cells has been studied.

(Fossgreen et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

APP695

(Xu et al., 2016)

Hsap\APP^695.Scer\UAS

Hsap\APP^695.UAS

Name Synonyms

Secondary FlyBase IDs

References (21)

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