FlyBase Allele Report: Dmel\polo[GL00014]

General Information

Symbol

Dmel\polo^GL00014

Species

D. melanogaster

Name

FlyBase ID

FBal0262505

Feature type

allele

Associated gene

Dmel\polo

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00014}

Also Known As

GL00014

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00014}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00014

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^695.UAS

(Peng et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

polo^GL00014 driven by Scer\GAL4^Appl.G1a does not significantly affect number of boutons or branches at the larval neuromuscular junction, locomotion (larval or adult) or lifespan. polo^GL00014 driven by Scer\GAL4^fru-NP0021 does not affect male courtship preference. polo^GL00014 driven by Scer\GAL4^GMR.PU does not affect eye development or cell cycle at the third instar larval eye disc.

(Peng et al., 2015)

Females heterozygous for polo^GL00014 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to polo^GL00014 males results in: no eggs; egg/embryonic phenotype: no vitellogenic egg chambers; oogenesis defect.

(Sopko et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

polo^GL00014, Scer\GAL4^NP0021 is a suppressor | partially of abnormal courtship behavior | male phenotype of Hsap\APP^695.UAS, Scer\GAL4^NP0021

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PU

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal eye color phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PU

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal mitotic cell cycle | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PU

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of short lived phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of wing phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of type Ib terminal bouton | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^Appl.G1a is a suppressor | partially of type Is terminal bouton | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PU

(Peng et al., 2015)

polo^GL00014, Scer\GAL4^GMR.PU is a suppressor | partially of eye disc | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PU

(Peng et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of polo^GL00014 partially suppresses the wing expansion defects, shorter lifespan, locomotion defects (larval and adult) and increases of bouton and branch number (including decreases in Ib boutons and increases in Is boutons) seen at the larval neuromuscular junction in animals with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a.

Co-expression of polo^GL00014 partially suppresses the courtship defects in male flies with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^fru-NP0021.

Co-expression of polo^GL00014 partially suppresses the adult eye development defects and abnormal cell cycle re-entry at the third instar larval eye disc seen when Hsap\APP^695.Scer\UAS is driven by Scer\GAL4^GMR.PU.

Adult-specific (reared at 17[o]C, 25[o]C after eclosion) expression of polo^GL00014 partially suppresses the decline in climbing ability seen in older flies expressing Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a during adulthood. Adult-specific (reared at 17[o]C, 29[o]C after eclosion) expression of polo^GL00014 partially suppresses shortened lifespan in flies with Hsap\APP^695.Scer\UAS driven by Scer\GAL4^Appl.G1a during adulthood.

(Peng et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

35146

y¹ sc^* v¹ sev²¹; P{TRiP.GL00014}attP2

NIG-Fly

GL00014