FlyBase Allele Report: Scer\GAL4[NP0021]

General Information

Symbol

Scer\GAL4^NP0021

Species

S. cerevisiae

Name

FlyBase ID

FBal0220176

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}fru^NP0021

Carried in Construct

Also Known As

fru-GAL4, NP21, fru^NP21-Gal4, NP21-GAL4, fru^NP21, P{GawB}NP21, NP0021-Gal4

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Ito et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{GawB}fru^NP0021

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:18,489,223..18,489,223 [-]

(Marini-Davis et al., 2025, Valentino et al., 2025, Zhang et al., 2025, Zhuravlev et al., 2024, Chen et al., 2023, Elya et al., 2023, Takayanagi-Kiya et al., 2023, Zhuang et al., 2023, Muria et al., 2021, Nojima et al., 2021, Tanaka et al., 2021, Moscato et al., 2020, Sato et al., 2020, Silva et al., 2020, Zhuang et al., 2020, Liu et al., 2019, Nojima et al., 2019, Olson et al., 2019, Sato et al., 2019, Flybrain Neuron Database et al., 2018.6.28, Scheunemann et al., 2018, Aranda et al., 2017, Chowdhury et al., 2017, Sethi and Wang, 2017, Grover et al., 2016, Huang et al., 2016, Ito et al., 2016, Yagi et al., 2016, Bouzaiane et al., 2015, Peng et al., 2015, Siegenthaler et al., 2015, Hu et al., 2014, Fujita et al., 2013, Ito et al., 2012, Tanaka et al., 2012, Goto et al., 2011, Pauls et al., 2010, Pauls et al., 2010, Potter et al., 2010, Lin et al., 2009, Sakurai et al., 2009, Kimura et al., 2008, Tanaka et al., 2008, Yu and Dickson, 2006, Zhu et al., 2006, Kimura et al., 2005, Tanaka et al., 2004, Ito et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage | male

brain | restricted

(Goto et al., 2011)

pupal stage P6 | male

larval ventral nerve cord | restricted

(Goto et al., 2011)

pupal stage P7

antennal lobe glomerulus DA1

(Sakurai et al., 2009)

adult stage | male

neuron | subset of adult brain

(Kimura et al., 2008)

adult fruitless aSP-b (male) neuron

Comment: referred to as aSP1

(Kimura et al., 2008)

adult fruitless aSP-a (male) neuron

Comment: referred to as aSP2

(Kimura et al., 2008)

adult fruitless aSP-f (male) neuron

Comment: referred to as aSP3a

(Kimura et al., 2008)

adult fruitless pSP-d neuron

Comment: referred to as aSP3b1

(Kimura et al., 2008)

adult fruitless aSP-g (male) neuron

Comment: referred to as aSP3b2

(Kimura et al., 2008)

adult fruitless aSP-k (male) neuron

Comment: referred to as aSP3c

(Kimura et al., 2008)

adult fruitless pIP-d neuron

Comment: referred to as Lv1+Ld

(Kimura et al., 2008)

adult fruitless pIP-b neuron

Comment: referred to as Lv2

(Kimura et al., 2008)

adult fruitless aDT-b (male) neuron

Comment: referred to as mAL

(Kimura et al., 2008)

adult fruitless aDT-d (male) neuron

Comment: referred to as AL1

(Kimura et al., 2008)

adult fruitless aDT-c neuron

Comment: referred to as AL2

(Kimura et al., 2008)

adult fruitless aDT-e (male) neuron

Comment: referred to as AL3

(Kimura et al., 2008)

adult fruitless aSP-i (male) neuron

Comment: referred to as AL5a

(Kimura et al., 2008)

adult fruitless aIP-c (male) neuron

Comment: referred to as AL5b and AL6b

(Kimura et al., 2008)

adult fruitless aIP-e (male) neuron

Comment: referred to as AL6a

(Kimura et al., 2008)

adult fruitless aDT-h neuron

Comment: referred to as mcALa

(Kimura et al., 2008)

adult fruitless M neuron

(Kimura et al., 2008)

adult fruitless Lo neuron

(Kimura et al., 2008)

adult fruitless P1 (male) neuron

Comment: referred to as P1

(Kimura et al., 2008)

descending neuron of the posterior brain DNp36 (male)

Comment: referred to as P2a and P2c

(Kimura et al., 2008)

adult fruitless pIP-a (male) neuron

Comment: referred to as P2b

(Kimura et al., 2008)

adult fruitless pMP-f (male) neuron

Comment: referred to as P2e

(Kimura et al., 2008)

adult fruitless pIP-e (male) neuron

Comment: referred to as P4a and P4b

(Kimura et al., 2008)

Additional Information

Statement

Reference

Drives expression in fruitless expressing neurons, including in male-specific fruitless neurons, in adults. Drives expression in the larval and adult mushroom bodies, and in a subset of pupal and adult antennal lobe glomeruli.

(FlyBase Curators, 2017)

ScerGAL4^fru-NP0021 drives expression in around 6 uniglomerular antennal lobe projection neurons. Their cell bodies are positioned in the lateral part of the antennal lobe and they project to antennal lobe glomerulus DA1 only. Expression is also detected in one ventrally located multiglomerular projection neuron which innervates glomeruli DA4l, VA1d, VA1v, VL1, VL2a, VM4, VM6, VC5, VP1, VP2, VP3 and VP4.

(Tanaka et al., 2012)

ScerGAL4^fru-NP0021 drives expression in a subset of neurons in the male pupal brain, including a cluster of neurons located dorsal and medially to the antennal lobe (mAL neurons). Expression is also observed in a subset of neurons in the ventral nerve cord. Almost all neurons that express ScerGAL4^fru-NP0021 also express hb protein. All neurons labelled by ScerGAL4^fru-NP0021 express fru protein.

(Goto et al., 2011)

Expression is observed in ~200 projection neurons in the γ lobe of the mushroom body.

(Tanaka et al., 2004)

Expressed in clusters of mushroom body neurons that project to the median and lateral bundles. Expression in the corpora pedunculata (mushroom body) includes all known substructures (but may not include all neurons).

(Ito et al., 1997)

Marker for

gamma Kenyon cell

(Tanaka et al., 2004)

Reflects expression of

fru

(Tanaka et al., 2012, Goto et al., 2011, Pauls et al., 2010, Potter et al., 2010, Sakurai et al., 2009, Kimura et al., 2008, Tanaka et al., 2008)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal copulation | male, with tei^EY11779

(Sato et al., 2020)

abnormal courtship behavior, with Hsap\APP^695.UAS

(Hu et al., 2014)

abnormal courtship behavior, with Hsap\HTT^{Q72.ex1p.UAS.EGFP}

(Hu et al., 2014)

abnormal courtship behavior, with NPF^KK111846

(Liu et al., 2019)

abnormal courtship behavior | male, with Hsap\APP^695.UAS

(Peng et al., 2015)

abnormal courtship behavior | male, with tei^EY11779

(Sato et al., 2020)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Bouzaiane et al., 2015)

abnormal neuroanatomy, with hb^JF03309

(Goto et al., 2011)

abnormal neuroanatomy | adult stage | female | somatic clone, with robo1^GD2608

(Ito et al., 2016)

abnormal neuroanatomy | adult stage | female | somatic clone, with tei^{RNAi.ex6-7.UAS}

(Sato et al., 2020)

abnormal neuroanatomy | somatic clone, with pum^RBD.UAS

(Goto et al., 2011)

abnormal neuroanatomy | somatic clone, with pum^UAS.fl

(Goto et al., 2011)

decreased efficacy of learning | adult stage, with LIMK1^UAS.Tag:HA

(Zhuravlev et al., 2024)

Phenotype Manifest In

adult fruitless aDT-b (female) neuron | adult stage | somatic clone, with tei^{RNAi.ex6-7.UAS}

(Sato et al., 2020)

adult fruitless aDT-h neuron | adult stage | female | somatic clone, with tei^{RNAi.ex6-7.UAS}

(Sato et al., 2020)

antennal lobe neuroblast | somatic clone, with pum^RBD.UAS

(Goto et al., 2011)

antennal lobe neuroblast | somatic clone, with pum^UAS.fl

(Goto et al., 2011)

neurite | adult stage | ectopic | female | somatic clone, with robo1^GD2608

(Ito et al., 2016)

neurite | adult stage | ectopic | somatic clone, with tei^{RNAi.ex6-7.UAS}

(Sato et al., 2020)

Detailed Description

Statement

Reference

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^NP0021 cells in adults significantly reduces short term (5 min) aversive olfactory memory, but not its anaesthesia-resistant component, compared to controls; inhibition during testing significantly reduces medium term (3 h) aversive olfactory memory, including its anaesthesia-resistant component, compared to controls, with no effect on long term (24 h after massed training) memory; inhibition does not affect olfactory acuity, compared to controls; inhibition (using shi^1.UAS at 33[o]C) of a subset of Scer\GAL4^NP0021 cells in adults (restricted using Scer\GAL80^Mef2.mb247) during testing does not affect medium term (3 h) aversive olfactory memory, compared to controls.

(Bouzaiane et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\APP^695.UAS, Scer\GAL4^NP0021 has abnormal courtship behavior | male phenotype, suppressible | partially by polo^HMS00530, Scer\GAL4^NP0021

(Peng et al., 2015)

Hsap\APP^695.UAS, Scer\GAL4^NP0021 has abnormal courtship behavior | male phenotype, suppressible | partially by polo^GL00014, Scer\GAL4^NP0021