FlyBase Insertion Report: Dmel\P{GawB}fru[NP0021]

General Information

Symbol

Dmel\P{GawB}fru^NP0021

Species

D. melanogaster

Name

FlyBase ID

FBti0033416

Feature type

transposable_element_insertion_site

Also Known As

fru-GAL4, NP21, fru^NP21-Gal4, NP21-GAL4, P{GawB}NP21, NP0021-Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

fru, Scer\GAL4

Viability / fertility

Associated allele(s)

fru^NP0021, Scer\GAL4^NP0021

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3R ( 91B2 )

Sequence Location

3R:18,489,223..18,489,223 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:18,489,223..18,489,223 [-]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

91B2 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG215261

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

fru

(Olson et al., 2019, Goto et al., 2011, Kimura et al., 2005)

Scer\GAL4

(Goto et al., 2011, Pauls et al., 2010, Potter et al., 2010, Lin et al., 2009, Sakurai et al., 2009, Tanaka et al., 2008, Yu and Dickson, 2006, Kimura et al., 2005, Tanaka et al., 2004)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

fru^NP0021

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^NP0021

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage | male

brain | restricted

(Goto et al., 2011)

pupal stage P6 | male

larval ventral nerve cord | restricted

(Goto et al., 2011)

pupal stage P7

antennal lobe glomerulus DA1

(Sakurai et al., 2009)

adult stage | male

neuron | subset of adult brain

(Kimura et al., 2008)

adult fruitless aSP-b (male) neuron

Comment: referred to as aSP1

(Kimura et al., 2008)

adult fruitless aSP-a (male) neuron

Comment: referred to as aSP2

(Kimura et al., 2008)

adult fruitless aSP-f (male) neuron

Comment: referred to as aSP3a

(Kimura et al., 2008)

adult fruitless pSP-d neuron

Comment: referred to as aSP3b1

(Kimura et al., 2008)

adult fruitless aSP-g (male) neuron

Comment: referred to as aSP3b2

(Kimura et al., 2008)

adult fruitless aSP-k (male) neuron

Comment: referred to as aSP3c

(Kimura et al., 2008)

adult fruitless pIP-d neuron

Comment: referred to as Lv1+Ld

(Kimura et al., 2008)

adult fruitless pIP-b neuron

Comment: referred to as Lv2

(Kimura et al., 2008)

adult fruitless aDT-b (male) neuron

Comment: referred to as mAL

(Kimura et al., 2008)

adult fruitless aDT-d (male) neuron

Comment: referred to as AL1

(Kimura et al., 2008)

adult fruitless aDT-c neuron

Comment: referred to as AL2

(Kimura et al., 2008)

adult fruitless aDT-e (male) neuron

Comment: referred to as AL3

(Kimura et al., 2008)

adult fruitless aSP-i (male) neuron

Comment: referred to as AL5a

(Kimura et al., 2008)

adult fruitless aIP-c (male) neuron

Comment: referred to as AL5b and AL6b

(Kimura et al., 2008)

adult fruitless aIP-e (male) neuron

Comment: referred to as AL6a

(Kimura et al., 2008)

adult fruitless aDT-h neuron

Comment: referred to as mcALa

(Kimura et al., 2008)

adult fruitless M neuron

(Kimura et al., 2008)

adult fruitless Lo neuron

(Kimura et al., 2008)

adult fruitless P1 (male) neuron

Comment: referred to as P1

(Kimura et al., 2008)

descending neuron of the posterior brain DNp36 (male)

Comment: referred to as P2a and P2c

(Kimura et al., 2008)

adult fruitless pIP-a (male) neuron

Comment: referred to as P2b

(Kimura et al., 2008)

adult fruitless pMP-f (male) neuron

Comment: referred to as P2e

(Kimura et al., 2008)

adult fruitless pIP-e (male) neuron

Comment: referred to as P4a and P4b

(Kimura et al., 2008)

Additional Information

Statement

Reference

Drives expression in fruitless expressing neurons, including in male-specific fruitless neurons, in adults. Drives expression in the larval and adult mushroom bodies, and in a subset of pupal and adult antennal lobe glomeruli.

(FlyBase Curators, 2017)

ScerGAL4^fru-NP0021 drives expression in around 6 uniglomerular antennal lobe projection neurons. Their cell bodies are positioned in the lateral part of the antennal lobe and they project to antennal lobe glomerulus DA1 only. Expression is also detected in one ventrally located multiglomerular projection neuron which innervates glomeruli DA4l, VA1d, VA1v, VL1, VL2a, VM4, VM6, VC5, VP1, VP2, VP3 and VP4.

(Tanaka et al., 2012)

ScerGAL4^fru-NP0021 drives expression in a subset of neurons in the male pupal brain, including a cluster of neurons located dorsal and medially to the antennal lobe (mAL neurons). Expression is also observed in a subset of neurons in the ventral nerve cord. Almost all neurons that express ScerGAL4^fru-NP0021 also express hb protein. All neurons labelled by ScerGAL4^fru-NP0021 express fru protein.

(Goto et al., 2011)

Expression is observed in ~200 projection neurons in the γ lobe of the mushroom body.

(Tanaka et al., 2004)

Expressed in clusters of mushroom body neurons that project to the median and lateral bundles. Expression in the corpora pedunculata (mushroom body) includes all known substructures (but may not include all neurons).

(Ito et al., 1997)

Marker for

gamma Kenyon cell

(Tanaka et al., 2004)

Reflects expression of

fru

(Tanaka et al., 2012, Goto et al., 2011, Pauls et al., 2010, Potter et al., 2010, Sakurai et al., 2009, Kimura et al., 2008, Tanaka et al., 2008)

Reporter construct used in assay

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2012, Goto et al., 2011, Pauls et al., 2010, Potter et al., 2010, Kimura et al., 2008, Tanaka et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

30027

w¹¹¹⁸; P{GawB}fru^NP0021

Kyoto

103496

y^* w^*; P{GawB}fru^NP0021 / TM6, P{UAS-lacZ.UW23-1}UW23-1

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG215261

Comments

Location 3R:14314944-14314945 refined by FlyBase alignment of dbGSS accession AG215261 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (28)

Reported As

Symbol Synonym

(Olson et al., 2019)

E0409

(Chen et al., 2023)

Fru-GAL4

(Marini-Davis et al., 2025)

Fru^NP21-Gal4

(Valentino et al., 2025)

GAL4-NP21

(Lin et al., 2009)

NP 21

(Pauls et al., 2010, Pauls et al., 2010)

NP0021

(Flybrain Neuron Database et al., 2018.6.28)

NP0021-GAL4

(Takayanagi-Kiya et al., 2023)

NP0021-Gal4

(Siegenthaler et al., 2015)

NP21

(Tanaka et al., 2021, Sato et al., 2020, Scheunemann et al., 2018, Yagi et al., 2016, Fujita et al., 2013, Ito et al., 2012, Tanaka et al., 2012, Pauls et al., 2010, Sakurai et al., 2009, Tanaka et al., 2008, Yu and Dickson, 2006, Kimura et al., 2005, Tanaka et al., 2004)

NP21 GAL4

(Bouzaiane et al., 2015)

NP21-GAL4

(Sethi and Wang, 2017, Potter et al., 2010)

NP21-Gal4

(Muria et al., 2021, Silva et al., 2020, Liu et al., 2019, Nojima et al., 2019, Siegenthaler et al., 2015)

P{GawB}NP0021

(FlyBase, 1992-)

P{GawB}NP0021-5-1

(FlyBase, 1992-)

P{GawB}NP21

(FlyBase, 2005, Tanaka et al., 2004, FlyBase, 1992-)

P{GawB}Np21

(Ito et al., 1997, FlyBase, 1992-)

P{GawB}fru^NP0021

(FlyBase, 1992-)

P{w^+mW.hs=GawB}fru^NP0021

(Scheunemann et al., 2018)

Tm26-Gal4

(Valentino et al., 2025)

fru-GAL4

(Zhang et al., 2025, Moscato et al., 2020, Sato et al., 2020, Sato et al., 2019, Aranda et al., 2017, Hu et al., 2014)

fru-Gal4

(Elya et al., 2023, Zhuang et al., 2023, Zhuang et al., 2020, Liu et al., 2019, Grover et al., 2016, Peng et al., 2015)

fru^NP21

(Goto et al., 2011)

fru^NP21-GAL4

(Moscato et al., 2020, Sato et al., 2020, Sato et al., 2019, Chowdhury et al., 2017)

fru^NP21-Gal4

(Grover et al., 2016, Huang et al., 2016, Ito et al., 2016, Kimura et al., 2008)

fruitless Gal4

(Flybrain Neuron Database et al., 2018.6.28)

fruitless-GAL4

(Hu et al., 2014)

γ neuron-specificGAL4-NP21

(Zhu et al., 2006)

Name Synonyms

Secondary FlyBase IDs

FBti0004175
FBti0058536

References (52)

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