FlyBase Allele Report: Dmel\fru[NP0021]

General Information

Symbol

Dmel\fru^NP0021

Species

D. melanogaster

Name

FlyBase ID

FBal0192053

Feature type

allele

Associated gene

Dmel\fru

Associated Insertion(s)

P{GawB}fru^NP0021

Carried in Construct

Also Known As

fru-GAL4, NP21, fru^NP21-Gal4, NP21-GAL4, fru^NP21, P{GawB}NP21, NP0021-Gal4

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Kimura et al., 2005)

Mutagen

P-element activity

(Kimura et al., 2005)

Progenitor genotype

Associated Insertion(s)

P{GawB}fru^NP0021

Cytology

Description

The P{GawB} insertion is within the second intron of fru.

(Kimura et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:18,489,223..18,489,223 [-]

(Olson et al., 2019, Goto et al., 2011, Kimura et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal copulation | male (with fru^0-1)

(Sato et al., 2020)

abnormal courtship behavior

(Kimura et al., 2005)

abnormal courtship behavior (with fru^sat)

(Kimura et al., 2005)

abnormal courtship behavior | male (with fru^0-1)

(Sato et al., 2020)

abnormal neuroanatomy | adult stage | male | somatic clone

(Ito et al., 2016)

abnormal neuroanatomy | male (with fru^sat)

(Kimura et al., 2008)

Phenotype Manifest In

adult brain & neuron | medial | male

(Kimura et al., 2005)

adult fruitless pMP-e (female) neuron (with fru^sat)

(Kimura et al., 2008)

eye, with Scer\GAL4^GMR.PF

(Goto et al., 2011)

muscle cell of muscle of Lawrence

(Kimura et al., 2005)

muscle cell of muscle of Lawrence (with fru^sat)

(Kimura et al., 2005)

neurite | adult stage | male | somatic clone

(Ito et al., 2016)

Detailed Description

Statement

Reference

fru^0-1/fru^NP0021 males exhibit reduced courtship activity and copulation success.

(Sato et al., 2020)

fru^NP0021/fru^ΔC (but not fru^NP0021/fru^ΔA or fru^NP0021/fru^ΔB) MARCM clones (in Scer\GAL4^fru-NP0021 neurons) fail to develop a male-specific mAL ipsilateral neurite.

(Ito et al., 2016)

Expression of fru^NP0021 in the developing eye under the control of Scer\GAL4^GMR.PF generates a rough eye phenotype.

fru^NP0021 heterozygotes exhibit an mAL cluster composed of 30 cells in males and 5 cells in females, as in controls.

(Goto et al., 2011)

The P1 neurite terminals in the contralateral hemisphere consistently fail to turn ventrally in fru^NP0021/fru^sat males.

(Kimura et al., 2008)

Homozygous fru^NP0021 , and fru^NP0021/fru^sat mutants exhibit a loss of the male specific muscle of Lawrence. Mutant also exhibit markedly reduced courtship behaviour towards males as well as females. fru^NP0021/fru^sat, fru^NP0021/fru¹, fru^NP0021/Df(3R)Cha7 males have on average 5 "neurons medially located, just above antennal lobe" (mALs), compared to ~30 in wild-type males. The number in females (~5) is unaffected. Homozygous fru^NP0021 males have no mALs with ipsolateral projections are observed, they have have contralateral, bifurcating projections (similar to the ones seen in females).

(Kimura et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fru^NP0021/fru^0-1 has abnormal courtship behavior | male phenotype, enhanceable by tei^EY11779/Scer\GAL4^NP0021

(Sato et al., 2020)

fru^NP0021/fru^0-1 has abnormal copulation | male phenotype, enhanceable by tei^EY11779/Scer\GAL4^NP0021

(Sato et al., 2020)

Other

Statement

Reference

Scer\GAL4^GMR.PF, Scer\GAL4^NP0021, fru^NP0021, hb^JF03309 has abnormal neuroanatomy | somatic clone phenotype

(Goto et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by Kr^If-1/Kr[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by ac[+]/ac⁴

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by Fmr1^Δ113M/Fmr1[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by Nedd8[+]/Nedd8^KG03071

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by eve³/eve[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by amos^Roi-1/amos[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by bib[+]/bib¹

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by eve[+]/eve¹

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by hb^UAS.cWa/Scer\GAL4^GMR.PF

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by neur[+]/neur¹¹

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by lea[+]/robo2^lea-2

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, enhanceable by ap^blt/ap[+]

(Goto et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, suppressible by hb¹²/hb[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, suppressible by EcR^M554fs/EcR[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, suppressible by bon^21B/bon[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, suppressible by dock[+]/dock⁰⁴⁷²³

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, suppressible by oaf[+]/oaf^KG00814

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, suppressible by Smr^BG01648/Smr[+]

(Goto et al., 2011)

Scer\GAL4^GMR.PF, fru^NP0021 has eye phenotype, suppressible by oaf[+]/oaf^Scim-a

(Goto et al., 2011)

Other

Statement

Reference

Scer\GAL4^NP0021, fru^NP0021, hb^JF03309 has antennal lobe neuroblast | somatic clone phenotype

(Goto et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A Kr^If-1 background strongly enhances the rough eye phenotype found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An ac⁴ background enhances the rough eye phenotype found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An eve³ background enhances the rough eye phenotype related to fru isoform A, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An amos^Roi-1 background enhances the rough eye phenotype found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A bib¹ background enhances the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An eve¹ background enhances the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A hb¹² heterozygous background strongly suppresses the rough eye phenotype found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

Simultaneous overexpression of hb^Scer\UAS.cWa and fru^NP0021 in the developing eye under the control of Scer\GAL4^GMR.PF disrupts the developing eye structure to a greater extent than expression of each transgene alone.

A neur¹¹ heterozygous background enhances the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A lea² background enhances the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An ap^blt background enhances the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An EcR^M554fs background suppresses the rough eye phenotype related to fru isoforms B and C, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A bon^21B background strongly suppresses the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A Fmr1^Δ113M background enhances the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A dock⁰⁴⁷²³ background suppresses the rough eye phenotype related to fru isoform A, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An oaf^KG00814 background suppresses the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A Smr^BG01648 background suppresses the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

A Nedd8^KG03071 background enhances the rough eye phenotype related to fru isoform B, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

An oaf^Scim-a background suppresses the rough eye phenotype related to fru isoform A, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

fru^NP0021 heterozygotes exhibit an mAL cluster composed of 30 cells in males and 5 cells in females, as in controls. Expression of hb^JF03309 in mAL neuroblast clones (using MARCM and Scer\GAL4^fru-NP0021, results in the ensemble of mAL neurons show intersexual characteristics; although the cluster retains male properties in that it is composed of 30 cells with the ipsilateral neurite, it also exhibits a female-like branching pattern of the contralateral neurite in the subesophageal ganglion. The contralateral neurite extending to the subesophageal ganglion is located more anteriorly in females than in males.

(Goto et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

30027