FlyBase Allele Report: Dmel\eve[3]

General Information

Symbol

Dmel\eve³

Species

D. melanogaster

Name

FlyBase ID

FBal0003885

Feature type

allele

Associated gene

Dmel\eve

Associated Insertion(s)

Carried in Construct

Also Known As

eve^R13

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Vincent et al., 1997, Cadigan et al., 1994, Harding et al., 1989, Tremml and Bienz, 1989)

loss of function allele

(Fujioka et al., 1999, Sackerson et al., 1999)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Fujioka et al., 1999)

Amino acid replacement: Q106term.

(Fujioka et al., 1999)

Mutation that is predicted to result in a truncated protein lacking the "recognition helix" (helix 3) of the homeodomain required for DNA binding, as well as the transcriptional repression domain.

(Fujioka et al., 1999)

Deletion causing a frameshift that replaces the C-terminal 134 amino acid residues with 79 extraneous residues.

(Frasch et al., 1988)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,979,801..9,979,801 [+]

Nucleotide change:

C9979801T

Amino acid change:

Q106term | eve-PA

(Fujioka et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion

(Paré et al., 2014)

abnormal cell polarity | gastrula stage

(Zallen and Wieschaus, 2004)

abnormal locomotor behavior | larval stage (with Df(2R)eve), with eve^ΔEL

(Marshall and Heckscher, 2022)

abnormal neuroanatomy

(Zarin et al., 2014)

abnormal neuroanatomy | larval stage, with eve^ΔEL

(Marshall and Heckscher, 2022)

abnormal planar polarity

(Paré et al., 2014)

lethal (with Df(2R)BSC158)

(Kahsai and Cook, 2018, Kahsai et al., 2016)

lethal (with Df(2R)X1)

(Kahsai and Cook, 2018, Kahsai et al., 2016)

lethal | recessive

(Fujioka et al., 1999, Sackerson et al., 1999)

pair rule phenotype, with Df(2R)eve, eve^EGNAY

(Kobayashi et al., 2001)

pair rule phenotype, with Df(2R)eve, eve^EGNPA

(Kobayashi et al., 2001)

pair rule phenotype, with Df(2R)eve, eve^EGNΔLFK

(Kobayashi et al., 2001)

pair rule phenotype, with eve^EGNAY

(Kobayashi et al., 2001)

pair rule phenotype, with eve^EGNPA

(Kobayashi et al., 2001)

pair rule phenotype, with eve^EGNΔLFK

(Kobayashi et al., 2001)

pair rule phenotype (with Df(2R)eve), with eve^EGNAY

(Kobayashi et al., 2001)

pair rule phenotype (with Df(2R)eve), with eve^EGNPA

(Kobayashi et al., 2001)

pair rule phenotype (with Df(2R)eve), with eve^EGNΔLFK

(Kobayashi et al., 2001)

Phenotype Manifest In

axon | embryonic stage 16, with eve^ΔEL

(Marshall and Heckscher, 2022)

axon collateral | larval stage, with eve^ΔEL

(Marshall and Heckscher, 2022)

cephalic furrow

(Crozatier et al., 1999, Vincent et al., 1997)

cephalic furrow | absent | embryonic stage 6

(Eritano et al., 2020)

dendritic branch | larval stage, with eve^ΔEL

(Marshall and Heckscher, 2022)

embryo, with Df(2R)eve, eve^EGNAY

(Kobayashi et al., 2001)

embryo, with Df(2R)eve, eve^EGNPA

(Kobayashi et al., 2001)

embryo, with Df(2R)eve, eve^EGNΔLFK

(Kobayashi et al., 2001)

embryo, with eve^EGNAY

(Kobayashi et al., 2001)

embryo, with eve^EGNPA

(Kobayashi et al., 2001)

embryo, with eve^EGNΔLFK

(Kobayashi et al., 2001)

embryo (with Df(2R)eve), with eve^EGNAY

(Kobayashi et al., 2001)

embryo (with Df(2R)eve), with eve^EGNPA

(Kobayashi et al., 2001)

embryo (with Df(2R)eve), with eve^EGNΔLFK

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle, with Df(2R)eve, eve^EGNAY

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle, with Df(2R)eve, eve^EGNPA

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle, with Df(2R)eve, eve^EGNΔLFK

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle, with eve^EGNAY

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle, with eve^EGNPA

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle, with eve^EGNΔLFK

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle (with Df(2R)eve), with eve^EGNAY

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle (with Df(2R)eve), with eve^EGNPA

(Kobayashi et al., 2001)

embryonic/first instar larval cuticle (with Df(2R)eve), with eve^EGNΔLFK

(Kobayashi et al., 2001)

embryonic/larval fat body

(Riechmann et al., 1998)

larval DA1 motor neuron

(Zarin et al., 2014)

larval EL neuron | embryonic stage 16, with eve^ΔEL

(Marshall and Heckscher, 2022)

larval intersegmental nerve

(Zarin et al., 2014)

larval RP2 motor neuron

(Zarin et al., 2014)

mitotic domain 1 | extended germ band stage

(Irvine and Wieschaus, 1994)

mitotic domain 2

(Vincent et al., 1997)

parasegment

(Kobayashi et al., 2001)

parasegment 1 | precursor

(Crozatier et al., 1999)

ventral denticle belt

(Harding et al., 1989, Tremml and Bienz, 1989)

ventral denticle belt, with Df(2R)eve, eve^EGNAY

(Kobayashi et al., 2001)

ventral denticle belt, with Df(2R)eve, eve^EGNPA

(Kobayashi et al., 2001)

ventral denticle belt, with Df(2R)eve, eve^EGNΔLFK

(Kobayashi et al., 2001)

ventral denticle belt, with eve^EGNAY

(Kobayashi et al., 2001)

ventral denticle belt, with eve^EGNPA

(Kobayashi et al., 2001)

ventral denticle belt, with eve^EGNΔLFK

(Kobayashi et al., 2001)

ventral denticle belt (with Df(2R)eve), with eve^EGNAY

(Kobayashi et al., 2001)

ventral denticle belt (with Df(2R)eve), with eve^EGNPA

(Kobayashi et al., 2001)

ventral denticle belt (with Df(2R)eve), with eve^EGNΔLFK

(Kobayashi et al., 2001)

Detailed Description

Statement

Reference

In eve^ΔEL/eve^ΔEL, eve³/eve³ larvae, EL interneurons branch excessively off the main neurite near the soma and in the midline, which is never seen in controls; the axon is less extended than in controls, branching at ~40 microns from the soma; the dorsally projecting dendrites are significantly reduced and the ventrally projecting dendrites are significant increased.

eve^ΔEL/eve^ΔEL, Df(2R)eve/eve³ larvae show decreased crawling ability.

(Marshall and Heckscher, 2022)

Cell intercalation is reduced by more than 30% in eve³ mutant embryos, accompanied by slower edge contraction.

(Paré et al., 2014)

aCC and RP2 fail to exit the CNS in eve³ mutants.

(Zarin et al., 2014)

Anterior-posterior polarization of cells in the extending germband is partially disrupted in eve³ homozygous embryos.

(Zallen and Wieschaus, 2004)

When eve³/- males are mated with heterozygous Gug^01323a mothers practically no embryos survive. These embryos die at late embryonic stage, they lose some or all of the even numbered ventral denticle belts. When the opposite cross (eve³/+ mother, Gug^01323a/+ fathers) no reduction in survival is seen.

(Zhang et al., 2002)

The cuticles of eve³ or eve³/Df(2R)eve embryos carrying eve^EGNΔLFK, eve^EGNAY or eve^EGNPA resembles that of an eve hypomorph, with pair-rule defects in the pattern of abdominal denticle bands. Heterozygous embryos have abnormally narrow odd-numbered parasegments at early stages of development.

(Kobayashi et al., 2001)

An ectopic stripe of early mitotic cells is seen at stage 8 in the presumptive parasegment 1 of eve³ mutant embryos. The cephalic furrow does not form.

(Crozatier et al., 1999)

The dorsolateral fat body is missing, the ventral fat body cluster is still present.

(Riechmann et al., 1998)

Homozygous embryos fail to form a cephalic furrow. The ventral furrow and proctodeal invagination form normally. No signs of initiator cell shortening are seen between the beginning of cycle 14 and stage 7. An irregular anterior fold is often seen in later stage embryos, at a variable position within mitotic domain 2 (MD2). This fold only arises after cells in MD2 have entered mitosis. The fold eventually resolves into a complete transversal furrow. MD2 is expanded posteriorly compared to wild-type embryos.

(Vincent et al., 1997)

Reduced rate of germ band extension.

(Irvine and Wieschaus, 1994)

Cuticular phenotype, lawn of dentical hairs.

(Harding et al., 1989)

The ftz stripes remain broad and do not sharpen at their anterior edges.

(Lawrence and Johnston, 1989)

Unsegmented 'lawn' phenotype of the cuticle. The arrays and patterns of mesodermal cells are disordered.

(Tremml and Bienz, 1989)

Unsegmented lawn of denticles.

(Jack et al., 1988)

strong allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

eve³ has abnormal neuroanatomy phenotype, enhanceable by unc-5[+]/unc-5⁸

(Zarin et al., 2014)

eve³ has abnormal neuroanatomy phenotype, enhanceable by unc-5[+]/beat-Ia^C163/beat-Ia[+]/unc-5⁸

(Zarin et al., 2014)

Suppressed by

Statement

Reference

eve³/eve[+], trol¹³ has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by CycE^hs.PR

(Park et al., 2001)

NOT suppressed by

Statement

Reference

eve³/eve[+], trol¹³ has lethal phenotype, non-suppressible by CycE^hs.PR

(Park et al., 2001)

Enhancer of

Statement

Reference

eve³ is an enhancer of lethal | embryonic stage | maternal effect phenotype of Gug^01323a

(Zhang et al., 2002)

eve³ is an enhancer of lethal | embryonic stage | maternal effect phenotype of Gug⁰³⁹²⁸

(Zhang et al., 2002)

eve³ is an enhancer of lethal | embryonic stage | maternal effect phenotype of Gug^e46-2

(Zhang et al., 2002)

eve³ is an enhancer of lethal | embryonic stage | maternal effect phenotype of Gug^rO154a

(Zhang et al., 2002)

eve³/eve[+] is an enhancer of decreased occurrence of cell division | larval stage phenotype of trol^d8

(Park et al., 2001)

eve³/eve[+] is an enhancer of decreased occurrence of cell division | larval stage phenotype of trol^p4

(Park et al., 2001)

eve³/eve[+] is an enhancer of partially lethal - majority die phenotype of trol¹³

(Park et al., 1998)

Other

Statement

Reference

eve³/eve[+], trol¹³ has lethal phenotype

(Park et al., 2001)

eve³/eve[+], trol¹³ has decreased occurrence of cell division | third instar larval stage phenotype

(Park et al., 2001)

eve³/eve[+], trol¹³ has decreased occurrence of cell division | larval stage phenotype

(Park et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

eve³ has larval intersegmental nerve phenotype, enhanceable by unc-5[+]/unc-5⁸

(Zarin et al., 2014)

eve³ has larval intersegmental nerve phenotype, enhanceable by unc-5[+]/beat-Ia^C163/beat-Ia[+]/unc-5⁸

(Zarin et al., 2014)

Suppressed by

Statement

Reference

eve³/eve[+], trol¹³ has larval brain & neuroblast phenotype, suppressible by CycE^hs.PR

(Park et al., 2001)

Enhancer of

Statement

Reference

eve³/eve[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, fru^NP0021

(Goto et al., 2011)

eve³/eve[+] is an enhancer of larval brain & neuroblast phenotype of trol^d8

(Park et al., 2001)

eve³/eve[+] is an enhancer of larval brain & neuroblast phenotype of trol^p4

(Park et al., 2001)

Other

Statement

Reference

Gug^01323a, eve³ has abdominal 4 ventral denticle belt phenotype

(Zhang et al., 2002)

Gug^01323a, eve³ has abdominal 8 ventral denticle belt phenotype

(Zhang et al., 2002)

Gug^01323a, eve³ has abdominal 2 ventral denticle belt phenotype

(Zhang et al., 2002)

Gug^01323a, eve³ has abdominal 6 ventral denticle belt phenotype

(Zhang et al., 2002)

eve³, trol¹³ has larval brain & neuroblast phenotype

(Park et al., 2001, Park et al., 1998)

eve³/eve[+], trol¹³ has larval brain & neuroblast phenotype

(Park et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

A transheterozygous combination of eve³/+, unc-5⁸/+ reduces beat-Ia levels to 50% and significantly increases the defects seen in intersegmental nerves, with the number of axons exhibiting stalling increasing from 8.1% to 23.1% in eve³/+, unc-5⁸/+ transheterozygotes and eve³/+, unc-5⁸/+, beat-Ia^C163/+ triple heterozygotes, respectively.

(Zarin et al., 2014)

An eve³ background enhances the rough eye phenotype related to fru isoform A, found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

(Goto et al., 2011)

(Zhang et al., 2002)

The degree of rescue of the eve³ embryonic phenotype conferred by one copy of eve^E+L.8.4 is reduced if the mother is heterozygous for gro^E48. The degree of rescue of the eve³ embryonic phenotype conferred by two copies of eve^EGNΔLFK is reduced slightly if the mother is heterozygous for gro^E48.

(Kobayashi et al., 2001)

The proliferation defect seen in trol^p4 animals is enhanced by one copy of eve³; 100% of animals show defective proliferation. The proliferation defect seen in trol^d8 animals is enhanced by one copy of eve³; 88% of animals show defective proliferation. 96% of trol¹³/Y ; eve³/+ animals show defective proliferation of larval brain neuroblasts. The addition of CycE^hs.PR (without heat shock) reduces the number of animals with defective proliferation to 35%, and expression of CycE^hs.PR using a 30 minute heat shock rescues the neuroblast phenotype almost completely. Weak induction of CycE^hs.PR does not rescue the enhanced lethality of trol¹³/Y ; eve³/+ animals. trol¹³/Y ; eve³/+ brains cultured in an explant assay in larval extract from eve³/+ or trol¹³/Y ; eve³/+ animals show a reduced amount of proliferation compared to wild-type brains cultured in larval extract from wild-type animals. In contrast, trol¹³/Y ; eve³/+ brains cultured in larval extract from wild-type animals have a level of proliferation close to that seen in controls (wild-type brains cultured in larval extract from wild-type animals). No trol¹³ brains cultured in larval extract from eve³/+ animals show normal proliferation. Addition of ecdysone rescues defective proliferation in trol¹³/Y ; eve³/+ brains cultured in larval extract from trol¹³/Y ; eve³/+ animals.

(Park et al., 2001)

Dominantly enhances the semi-lethality of trol¹³/Y males and shows a transheterozygous effect in trol¹³/+ females, resulting in a reduction in viability. This enhancement is repressed by eve^tPa. Hemizygous trol¹³ male larvae have a normal number of S phase neuroblasts per brain lobe. If the larvae are also heterozygous for eve³ then the number of S phase neuroblasts per brain lobe is reduced.

(Park et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

eve³ is rescued by eve^-6.6.+9.8

(Ludwig et al., 2011)

eve³ is rescued by eve^{MSE.-6.6.+9.8.SYFP2}

(Ludwig et al., 2011)

eve³ is rescued by eve^{INV-MSE.-6.6.+9.8.SYFP2}

(Ludwig et al., 2011)

eve³ is rescued by eve^ΔS2E/eve^Dpse\eve.S2E

(Ludwig, 2005)

eve³ is rescued by eve^eve.S2E/eve^ΔS2E

(Ludwig, 2005)

eve³ is rescued by eve^ΔS2E/eve^Dyak\eve.S2E

(Ludwig, 2005)

eve³/Df(2R)eve is rescued by eve^E+L.8.4

(Kobayashi et al., 2001)

eve³ is rescued by eve^E+L.8.4

(Kobayashi et al., 2001)

eve³ is rescued by eve^tPa

(Park et al., 1998)

Partially rescued by

eve³ is partially rescued by eve^{-6.6.+9.8.SYFP2}

(Ludwig et al., 2011)

eve³/Df(2R)eve is partially rescued by eve^EGNHA

(Kobayashi et al., 2001)

eve³ is partially rescued by eve^EGNHA

(Kobayashi et al., 2001)

eve³ is partially rescued by eve^E+L.8.4

(Fujioka et al., 1999)

eve³ is partially rescued by eve^E+L.9.2

(Fujioka et al., 1999)

eve³ is partially rescued by eve^E+L.8.4

(Sackerson et al., 1999)

Not rescued by

eve³ is not rescued by eve^S2E.0/eve^ΔS2E

(Ludwig, 2005)

eve³ is not rescued by eve^ΔS2E/eve^Dere\eve.S2E

(Ludwig, 2005)

eve³/Df(2R)eve is not rescued by eve^EGNPA

(Kobayashi et al., 2001)

eve³/Df(2R)eve is not rescued by eve^EGNΔLFK

(Kobayashi et al., 2001)

eve³/Df(2R)eve is not rescued by eve^EGNAY

(Kobayashi et al., 2001)

eve³ is not rescued by eve^EGNPA

(Kobayashi et al., 2001)

eve³ is not rescued by eve^EGNΔLFK

(Kobayashi et al., 2001)

eve³ is not rescued by eve^EGNAY

(Kobayashi et al., 2001)

Comments

Homozygosity for P{S2E.wt.eve.wt}attP2 rescues the viability of eve³ homozygotes to adulthood at a frequency of 80-85% (this is not significantly different from full 100% rescue), while the rescue frequency is lower in eve³ homozygotes carrying one copy of P{S2E.wt.eve.wt}attP2. There is no significant difference in the observed recovery of males versus females.

Homozygosity for P{S2E.wt.eve.YFP}attP2 rescues the viability of eve³ homozygotes to adulthood in more than 50% of cases.

Flies homozygous for P{S2E.MSE.eve.YFP}attP2 in a eve³ background are highly viable and fertile. However flies heterozygous for P{S2E.MSE.eve.YFP}attP2 show on average less than 5% rescue of eve³ to adulthood. In both cases there is a reduction in the recovery of males compared to females.

Flies homozygous for P{S2E.INV-MSE.eve.YFP}attP2 in a eve³ background are highly viable and fertile. However flies heterozygous for P{S2E.INV-MSE.eve.YFP}attP2 show on average less than 5% rescue of eve³ to adulthood.

(Ludwig et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (2)

299

105845

Notes on Origin

Discoverer

Associated with: recessive mutations that affect fertility, as revealed in the rescued condition using eve^E+L.8.4 or eve^E+L.9.2.

(Fujioka et al., 1999)

Comments

Null mutation.

(Fujioka et al., 1999)

The expression of l(1)sc is altered during stage 9 in homozygous mutant embryos.

(Martin-Bermudo et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

eve(3)

(Marshall and Heckscher, 2022)

eve³

(Kahsai and Cook, 2018, Zarin et al., 2014, Ali-Murthy et al., 2013, Goto et al., 2011, Schroeder et al., 2011, Fujioka et al., 2005)

eve^R13

(Dey et al., 2025, Eritano et al., 2020, Paré et al., 2014, Spahn et al., 2014, Manu et al., 2013, Webber et al., 2013, Ludwig et al., 2011, Ludwig, 2005, Jaynes and Fujioka, 2004, Zallen and Wieschaus, 2004, Fujioka et al., 2003, Fujioka et al., 2002, Zhang et al., 2002, Blankenship and Wieschaus, 2001, San Martin et al., 2001, Bouchard et al., 2000, Crozatier et al., 1999, Fujioka et al., 1999, Sackerson et al., 1999, Riechmann et al., 1998, Vincent et al., 1997, Poux et al., 1996, Colas et al., 1995, Harbecke and Lengyel, 1995, Baumgartner et al., 1994, Cadigan et al., 1994, Irvine and Wieschaus, 1994, Macias et al., 1994, Muller and Bienz, 1992, Martin-Bermudo et al., 1991, Jack and McGinnis, 1990, Harding et al., 1989, Lawrence and Johnston, 1989, Lawrence and Johnston, 1989, Tremml and Bienz, 1989, Frasch et al., 1988, Tearle and Nusslein-Volhard, 1987)

eve^r13

(Riechmann et al., 1997, Jack et al., 1988)

Name Synonyms

Secondary FlyBase IDs

References (53)

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