FlyBase Allele Report: Dmel\Tom40[JF02030]

General Information

Symbol

Dmel\Tom40^JF02030

Species

D. melanogaster

Name

FlyBase ID

FBal0220011

Feature type

allele

Associated gene

Dmel\Tom40

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02030}

Also Known As

Tom40 RNAi, UAS-Tom40 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

(Perkins et al., 2009.1.15)

Progenitor genotype

Carried in construct

P{TRiP.JF02030}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.1.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02030

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Liu et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Toll-6-D42

(Liu et al., 2018)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

decreased cell size | third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

lethal - all die before end of larval stage, with Scer\GAL4^elav.PLu

(Liu et al., 2018)

Phenotype Manifest In

autophagosome | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

fat cell | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

lysosome | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

mitochondrion | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

mitochondrion | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

omegasome | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

rhabdomere of eye photoreceptor cell | adult stage | progressive, with Scer\GAL4^GMR.PU

(Liu et al., 2018)

Detailed Description

Statement

Reference

Expressing Tom40^JF02030 under the control of Scer\GAL4^Cg.PA leads to severe mitochondrial defects in the third instar larval fat body, as shown by the absence of MitoGFP signal.

(Zhou et al., 2019)

The expression of Tom40^JF02030 under the control of Scer\GAL4^Cg.PA results in early third instar larval fat bodies with severely reduced cell sizes and dramatic accumulation of autophagosome-like structures (Atg8a-, Atg6-, Atg14-, Atg5-, Atg16-, Atg12-, Syx17-positive) wrapping ubiquitinated protein aggregates, omegasomes (ZFYVE1-positive) and lysosomes (Lamp1-positive), as compared to controls; in TEM, mitochondria are small and the double membranes of autophagosome-like structures enclose electron-dense aggregates and are surrounded by many lysosomes, as compared to controls.

The expression of Tom40^JF02030 under the control of Scer\GAL4^GMR.PU (together with Dicer-2, for efficient RNAi) leads to progressive eye defects/neurodegeneration (normal eyes in 2 days old adults; loss of rhabdomeres in 30 days old adults), as compared to controls.

The expression of Tom40^JF02030 under the control of Scer\GAL4^Toll-6-D42 (together with Dicer-2, for efficient RNAi) induces climbing defects in adults, as compared to controls.

(Liu et al., 2018)

Expression of Tom40^JF02030 under the control of Scer\GAL4^ple.PF results in a loss of dopaminergic (DA) neurons from the PPL1 cluster accompanied by mitochondrial aggregations in the DA neurons in the adult brain but expression under the Scer\GAL4^Mhc.PU driver has no adverse effect on adult wing posture or jumping ability.

(Gehrke et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Atg9^HMS01246, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Atg12^JF02704, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Atg5^JF02703, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Atg6^HMS01483, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Rab7^JF02377, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by HDAC6^UAS.cDa, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Nmnat^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/ref(2)P^HMS00938

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by park^HMS01800/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by park^UAS.Tag:V5/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Pink1^{D501A.UAS.Tag:V5}, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/park^HMS01800, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/Atg6^HMS01483, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/Syx17^JF01937, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by porin^JF03251/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Atg8a^HMS01328, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has decreased cell size | third instar larval stage phenotype, non-suppressible by Atg16^HMS01347, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^ple.PF, Tom40^JF02030 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Scer\GAL4^ple.PF, Tom40^JF02030 has decreased cell number | adult stage phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Enhancer of

Statement

Reference

Tom40^JF02030/Scer\GAL4^Mhc.PU is an enhancer of visible | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030, Scer\GAL4^Mhc.PU is an enhancer of visible | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

NOT Suppressor of

Statement

Reference

Tom40^JF02030/Scer\GAL4^Mhc.PU is a non-suppressor of abnormal jumping phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030/Scer\GAL4^ple.PF is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030/Scer\GAL4^ple.PF is a non-suppressor of decreased cell number | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Tom40^JF02030 has rhabdomere of eye photoreceptor cell | adult stage | progressive phenotype, enhanceable by Hsap\HTT^{72Q.UAS.Emerald}, Scer\GAL4^GMR.PU

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, enhanceable by Scer\GAL4^Cg.PA/Syx17^JF01937

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, enhanceable by Scer\GAL4^Cg.PA/Syx17^JF01937

(Liu et al., 2018)

Scer\GAL4^GMR.PU, Tom40^JF02030 has rhabdomere of eye photoreceptor cell | adult stage | progressive phenotype, enhanceable by Pink1^UAS.cCa, Scer\GAL4^GMR.PU

(Liu et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible by Atg9^HMS01246, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible by Atg5^JF02703, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible by Atg16^HMS01347, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible by Atg6^HMS01483, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible by Atg12^JF02704, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by Atg9^HMS01246, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by Atg6^HMS01483, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by Atg12^JF02704, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by Atg5^JF02703, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by Atg16^HMS01347, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Cg.PA/ref(2)P^HMS00938

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by Pink1^UAS.cCa, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, suppressible | partially by park^UAS.Tag:V5/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Cg.PA/ref(2)P^HMS00938

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible | partially by Pink1^UAS.cCa, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, suppressible | partially by park^UAS.Tag:V5/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^GMR.PU, Tom40^JF02030 has rhabdomere of eye photoreceptor cell | adult stage | progressive phenotype, suppressible | partially by ref(2)P^HMS00938/Scer\GAL4^GMR.PU

(Liu et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by porin^JF03251/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by porin^JF03251/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by Atg8a^HMS01328, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by Rab7^JF02377, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by Rab7^JF02377, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by Pink1^{D501A.UAS.Tag:V5}, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by Nmnat^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by HDAC6^UAS.cDa, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by HDAC6^UAS.cDa, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by Pink1^{D501A.UAS.Tag:V5}, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by Nmnat^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by park^HMS01800/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by park^HMS01800/Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/park^HMS01800, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/Atg5^JF02703, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has fat cell | early third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/Syx17^JF01937, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/Atg5^JF02703, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^Cg.PA, Tom40^JF02030 has autophagosome | early third instar larval stage phenotype, non-suppressible by Pink1^UAS.cCa/Syx17^JF01937, Scer\GAL4^Cg.PA

(Liu et al., 2018)

Scer\GAL4^ple.PF, Tom40^JF02030 has dopaminergic PPL1 neuron | adult stage phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Enhancer of

Statement

Reference

Tom40^JF02030, Scer\GAL4^GMR.PU is an enhancer of rhabdomere of eye photoreceptor cell | adult stage | progressive phenotype of Hsap\HTT^{72Q.UAS.Emerald}, Scer\GAL4^GMR.PU

(Liu et al., 2018)

Tom40^JF02030/Scer\GAL4^Cg.PA is an enhancer of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Syx17^JF01937

(Liu et al., 2018)

Tom40^JF02030/Scer\GAL4^Cg.PA is an enhancer of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Syx17^JF01937

(Liu et al., 2018)

Tom40^JF02030/Scer\GAL4^Mhc.PU is an enhancer of wing | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030/Scer\GAL4^Mhc.PU is an enhancer of flight muscle cell phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030/Scer\GAL4^Mhc.PU is an enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030/Scer\GAL4^ple.PF is an enhancer of dopaminergic neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030/Scer\GAL4^ple.PF is an enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tom40^JF02030, Scer\GAL4^Mhc.PU is an enhancer of wing | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Tom40^JF02030, Scer\GAL4^Mhc.PU is an enhancer of flight muscle cell phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Tom40^JF02030, Scer\GAL4^Mhc.PU is an enhancer of mitochondrion | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

NOT Suppressor of

Statement

Reference

Tom40^JF02030/Scer\GAL4^ple.PF is a non-suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Other

Statement

Reference

Pink1^UAS.cCa, Scer\GAL4^GMR.PU, Tom40^JF02030 has eye photoreceptor cell phenotype

(Liu et al., 2018)

Pink1^UAS.cCa, Scer\GAL4^GMR.PU, Tom40^JF02030 has autophagosome | adult stage phenotype

(Liu et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The progressive eye defects induced by the expression of Tom40^JF02030 under the control of Scer\GAL4^GMR.PU (normal eyes in 2 days old adults; loss of rhabdomeres in 30 days old adults) are enhanced by the co-expression of Pink1^UAS.cCa : even the 2-days-old fly eyes start to display small rhabdomeres and the photoreceptor cells are filled with large autophagosome-like structures.

(Liu et al., 2018)

The loss of dopaminergic PPL1 neurons observed in adult flies expressing Tom40^JF02030 RNAi under the control of Scer\GAL4^ple.PF is not affected by co-expression of Pink1^Scer\UAS.cYa.

The abnormal mitochondrial aggregations (observed in flight muscles or dopaminergic neurons), wing posture and jumping ability defects as well as the loss of dopaminergic neurons from the PPL1 cluster characteristic for Pink1^B9 adult homozygotes are exacerbated or not suppressed by expression (controlled by either the Scer\GAL4^Mhc.PU muscle driver or the Scer\GAL4^ple.PF neuronal driver) of Tom40^JF02030. Similarly, the mitochondrial aggregations in flight muscles along with the wing posture and jumping ability defects observed in adult flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PU are further enhanced by expression of Tom40^JF02030.