Open Close
General Information
Symbol
Dmel\Atg6HMS01483
Species
D. melanogaster
Name
FlyBase ID
FBal0263391
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Atg6RNAi, Atg6 RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-only expression of Atg6HMS01483 under the control of Scer\GAL4esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

The expression of Atg6HMS01483 under the control of Scer\GAL4Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls. Under standard conditions, there is no change in the number of autophagosome-like structures (Atg8a-positive), as compared to controls.

Expression of Atg6HMS01483 under the control of Scer\GAL4Myo31DF-NP0001 results in delayed midgut removal after pupariation, with increased size of the gastric caeca 4 hrs after puparium formation, but no difference in autophagy levels 2 hrs after puparium formation, as compared to controls.

Embryos derived from females expressing Atg6HMS01483 under the simultaneous control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 show a significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type.

Expression of Atg6HMS01483 under the control of Scer\GAL4Mef2.PR is able to significantly suppress the formation of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (10)