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General Information
Symbol
Dmel\Pink1UAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Clark
FlyBase ID
FBal0196298
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-pink1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Pink1.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Pink1UAS.cCa under the control of Scer\GAL4Cg.PA does not seem to affect mitochondria size in the third instar larval fat body (as assessed by MitoGFP).

Expressing Pink1UAS.cCa under the control of Scer\GAL4Mef2.PR leads to a small but significant decrease in adult climbing ability; adult thorax muscles do not show obvious signs of degeneration. In 3-day old adults, muscle mitochondria do not show obvious defects; in 35-days old adults, however, mitochondria are slightly smaller, rounder and/or of irregular size, and cristae exhibit mild abnormalities.

The expression of Pink1UAS.cCa under the control of Scer\GAL4Cg.PA does not affect the number of autophagosome-like structures (Atg8a-positive) in the early third instar larval fat body, as compared to controls; in TEM, the mitochondria number and morphology are not dramatically changed, as compared to controls.

The expression of Pink1UAS.cCa under the control of Scer\GAL4GMR.PU does not lead to eye defects (rhabdomere number in both 2- and 30-days old adults), as compared to controls.

The expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4Act.PU in clones within mosaic midguts results in enterocytes exhibiting a significant decrease in the relative mitochondrial content during pupariation, as compared to non-clone enterocytes; expression under the control of Scer\GAL4esg.PU results in a significant decrease in the relative mitochondrial content of midgut intestinal stem cells, from late third instar larval until afer puparium formation, but not at early third instar larval stage or adult stage, as compared to controls.

Expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4GMR.PF at 18[o]C results in a mild rough eye phenotype. A rough eye phenotype is also seen at 29[o]C.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology (enlarged clustered mitochondria) and functional (loss of inner mitochondrial potential measured using cationic fluorescent dye JC-1) defects in adult indirect flight muscles characteristic for animals expressing cluGD13926 under the control of Scer\GAL4Mef2.PR can be suppressed by co-expression of Pink1Scer\UAS.cCa.

Expression of HtrA2Scer\UAS.cYa enhances the rough eye phenotype seen when Pink1Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C.

Expression of HtrA2S266A.Scer\UAS does not enhance the rough eye phenotype seen when Pink1Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C.

Expression of HtrA2dsRNA.Scer\UAS.cYa fails to suppress the rough eye phenotype seen when Pink1Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PF at 29[o]C.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4Mef2.PR completely rescues the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink15 mutants.

Expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4Mef2.PR rescues the mitochondria morphology defects seen in Pink15 mutant indirect flight muscles.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Pink1Scer\UAS.cCa
Pink1UAS.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Clark
Secondary FlyBase IDs
    References (13)