FlyBase Allele Report: Dmel\clu[GD13926]

General Information

Symbol

Dmel\clu^GD13926

Species

D. melanogaster

Name

FlyBase ID

FBal0205871

Feature type

allele

Associated gene

Dmel\clu

Associated Insertion(s)

Carried in Construct

P{GD13926}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13926}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13926

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Pink1^RNAi.UAS.cYa

(Sen et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav-C155

(Wang et al., 2016)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Mef2.PR

(Wang et al., 2016)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die, with Scer\GAL4^Mef2.PR

(Wang et al., 2016)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Mhc.PW

(Sen et al., 2015)

visible, with Scer\GAL4^Tub.PU

(Sen et al., 2015)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

mitochondrion | adult stage, with Scer\GAL4^Mef2.PR

(Wang et al., 2016)

wing, with Scer\GAL4^Mhc.PW

(Sen et al., 2015)

wing, with Scer\GAL4^Tub.PU

(Sen et al., 2015)

Detailed Description

Statement

Reference

Expression of clu^GD13926 under the control of either Scer\GAL4^Mef2.PR or Scer\GAL4^elav-C155 impairs larval locomotion (reduced crawling speed compared to wild-type). Scer\GAL4^Mef2.PR-driven expression is partially lethal (only few escapers manage to eclose) and leads to mitochondrial morphology as well as functional defects (enlarged clustered mitochondria with reduced inner mitochondrial membrane potential) in adult indirect flight muscles

(Wang et al., 2016)

Expression of clu^GD13926 driven by Scer\GAL4^Mhc.PW or Scer\GAL4^tub.PU significantly increases abnormal wing posture compared to controls.

(Sen et al., 2015)

Adults expressing clu^GD13926 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing clu^GD13926 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 100% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13926

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Mhc.PW, clu^GD13926 has visible phenotype, enhanceable by Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Sen et al., 2015)

Scer\GAL4^Tub.PU, clu^GD13926 has visible phenotype, enhanceable by Scer\GAL4^Tub.PU/park^KK107919

(Sen et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Pink1^UAS.cCa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, suppressible by Pink1^UAS.cCa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Scer\GAL4^Mef2.PR/park^UAS.cGa

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, suppressible by Scer\GAL4^Mef2.PR/park^UAS.cGa

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Marf^GD11094, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, suppressible | partially by Marf^GD11094, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Drp1^UAS.cDa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, suppressible | partially by Drp1^UAS.cDa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by TER94^UAS.cRb/Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, suppressible | partially by TER94^UAS.cRb/Scer\GAL4^Mef2.PR

(Wang et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, non-suppressible by Cat^UAS.cAa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, non-suppressible by Cat^UAS.cAa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Mef2.PR/Sod1^UAS.cWa

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, non-suppressible by Scer\GAL4^Mef2.PR/Sod1^UAS.cWa

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has abnormal locomotor behavior | third instar larval stage phenotype, non-suppressible by Sod2^UAS.cMa/Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has partially lethal - majority die phenotype, non-suppressible by Sod2^UAS.cMa/Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Enhancer of

Statement

Reference

clu^GD13926, Scer\GAL4^Mhc.PW is an enhancer of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Sen et al., 2015)

clu^GD13926/Scer\GAL4^Tub.PU is an enhancer of visible phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Sen et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Mhc.PW, clu^GD13926 has wing phenotype, enhanceable by Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Sen et al., 2015)

Scer\GAL4^Tub.PU, clu^GD13926 has wing phenotype, enhanceable by Scer\GAL4^Tub.PU/park^KK107919

(Sen et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, suppressible by Scer\GAL4^Mef2.PR/park^UAS.cGa

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, suppressible by Marf^GD11094, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, suppressible by Drp1^UAS.cDa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, suppressible | partially by TER94^UAS.cRb/Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, suppressible by Pink1^UAS.cCa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, non-suppressible by Cat^UAS.cAa, Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, non-suppressible by Scer\GAL4^Mef2.PR/Sod1^UAS.cWa

(Wang et al., 2016)

Scer\GAL4^Mef2.PR, clu^GD13926 has mitochondrion | adult stage phenotype, non-suppressible by Sod2^UAS.cMa/Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Enhancer of

Statement

Reference

clu^GD13926, Scer\GAL4^Mhc.PW is an enhancer of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Sen et al., 2015)

clu^GD13926/Scer\GAL4^Tub.PU is an enhancer of wing phenotype of Scer\GAL4^Tub.PU, park^KK107919

(Sen et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology defects in adult indirect flight muscles (enlarged clustered mitochondria) characteristic for animals expressing clu^GD13926 under the control of Scer\GAL4^Mef2.PR can be suppressed to a varying degree by co-expression of any of the following: Pink1^Scer\UAS.cCa, park^Scer\UAS.cGa, Marf^GD11094, Drp1^UAS.cDa or TER94^UAS.cRb but not that of Cat^Scer\UAS.cAa, Sod1^UAS.cWa or Sod2^Scer\UAS.cMa. Co-expression of Pink1^Scer\UAS.cCa or park^Scer\UAS.cGa also rescues the loss of inner mitochondrial potential observed in the knock-down adults (measured using cationic fluorescent dye JC-1).

(Wang et al., 2016)

Co-expression of Pink1^{dsRNA.Scer\UAS} or park^KK107919 increases the percentage of abnormal wing posture seen in flies with clu^GD13926 driven by Scer\GAL4^Mhc.PW or Scer\GAL4^tub.PU, respectively.

(Sen et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v42136