The wing posture defects induced by the expression of Stoml2GD16706 under the control of Scer\GAL4fln.IFM alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, are fully suppressed by the co-expression of parkUAS.cGa.
The decreased adult climbing capacity observed upon the expression of Stoml2GD16706 under the control of Scer\GAL4ple.PU alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, is fully suppressed by the co-expression of parkUAS.cGa.
The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology (enlarged clustered mitochondria) and functional (loss of inner mitochondrial potential measured using cationic fluorescent dye JC-1) defects in adult indirect flight muscles characteristic for animals expressing cluGD13926 under the control of Scer\GAL4Mef2.PR can be suppressed by co-expression of parkScer\UAS.cGa.
The enlarged or aggregated mitochondria phenotype observed in the body wall muscles of Pink1B9 hemizygous male third instar larvae is suppressed either by combination with mask10.22/maskEY13048 alleles or by ectopic expression of parkScer\UAS.cGa under the control of Scer\GAL4how-24B.
Expression of parkScer\UAS.cGa under the control of Scer\GAL4da.G32 significantly suppresses the penetrance of the indented thorax phenotype seen in Pink1B9 mutants. This suppression is reduced if the flies are also carrying either ref(2)Pod2 or ref(2)Pod3.
The ability of parkScer\UAS.cGa expressed under the control of Scer\GAL4da.G32 to suppress the Pink1B9 mutant phenotypes of an indented thorax and reduced climbing ability is suppressed if the flies are also mutant for Atg1Δ3D.
Co-expression of Pink1Scer\UAS.T:Ivir\HA1 and parkScer\UAS.cGa under the control of Scer\GAL4GMR.PF results in a severe rough eye phenotype, which greatly exceeds that conferred when Pink1Scer\UAS.T:Ivir\HA1 is expressed alone. This phenotype is not suppressed in a HtrA2Δ1 mutant background.
Expression of parkScer\UAS.cGa enhances the rough eye phenotype seen when rho-7Scer\UAS.cWa is expressed under the control of Scer\GAL4GMR.PF, resulting in severe loss of eye tissue. This phenotype is not suppressed by HtrA2Δ1.
The indirect flight muscles of 2 day old Pink15 adults that are also expressing parkScer\UAS.cGa under the control of Scer\GAL4how-24B have some mitochondria with normal densely packed cristae and some with fragmented cristae, in contrast to 2 day old Pink15 single mutants, where the mitochondria of the indirect flight muscles have fragmented cristae.
Co-expression of parkScer\UAS.cGa rescues the age-dependent defect in climbing ability seen in flies expressing Rnor\Otcd.Scer\UAS under the control of Scer\GAL4da.G32. The defects in indirect flight muscle structure and mitochondrial integrity are also suppressed.
Co-expression of parkScer\UAS.cGa is no longer able to suppress the defects in climbing ability and indirect flight muscle structure seen in flies expressing Rnor\Otcd.Scer\UAS under the control of Scer\GAL4da.G32 when the flies are also mutant for Atg1Δ3D.
Expression of parkScer\UAS.cGa with a global (Scer\GAL4Act5C.PU) or neuronal (Scer\GAL4elav.PLu), but not muscle (Scer\GAL4G14), driver rescues locomotion in park25/parkZ3678 larvae; global and neuronal (but not muscle) expression partially rescues locomotion in park25/park25 larvae; neuronal but not muscle driver rescues contraction frequency in park25/park25 larvae; muscle expression rescues muscle resting membrane potential but not synaptic potential in park25/parkZ3678 larvae; neuronal expression partially rescues synaptic potential but not muscle resting membrane potential in park25/parkZ3678 larvae; global, neuronal and muscle drivers rescue synaptic overgrowth in park25/park25 larvae.
The addition of parkScer\UAS.cGa driven by Scer\GAL4how-24B or Scer\GAL4Mef2.PR rescues the flight and climbing behaviour defects seen in park13/Df(3L)Pc-MK animals. parkScer\UAS.cGa driven by Scer\GAL4how-24B rescues the mitochondrial and myofibril defects seen in park25/Df(3L)Pc-MK indirect flight muscles.