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General Information
Symbol
Dmel\parkUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Greene
FlyBase ID
FBal0146935
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-parkin, UAS-park
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt Sequences drive expression of a park cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

While expression of parkScer\UAS.cGa ameliorates the behavioral phenotypes seen in Pink1B9 mutants, it achieves this without restoring complex I activity.

Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ubiquitous expression of parkScer\UAS.cGa throughout development and adulthood under the control of Scer\GAL4da.Switch.PT (and treatment with RU486) results in an increase in the mean and maximum lifespans of female flies. Smaller effects are also seen in male flies. These long lived flies display normal food consumption and an increase in spontaneous physical activity in young flies. Young flies also show an increase in fecundity. Hypoxia resistance is similar to controls, but improved survival is seen following starvation. Expressing parkScer\UAS.cGa only during development has no major impact on adult longevity, whereas Scer\GAL4da.Switch.PT>parkScer\UAS.cGa flies are significantly longer lived when exposed to RU486 exclusively in the adult stage.

Ubiquitous expression of parkScer\UAS.cGa under the control of Scer\GAL4da.Switch.PT results in reduced levels of protein aggregates during ageing compared to controls. The average size of aggregates is also significantly reduced.

The mitochondria of young and aged flies expressing parkScer\UAS.cGa under the control of Scer\GAL4da.Switch.PT are fragmented (smaller and rounder) compared to controls. There is an increase in citrate synthase and mitochondrial respiratory complexes I and II activities in young flies, and an increase in citrate synthase activity in aged flies.

Constitutive expression of parkScer\UAS.cGa in neurons under the control of Scer\GAL4elav.Switch.PO (and treatment with RU486) results in an increase in the mean and maximum lifespans of female flies. An increase in lifespan is also seen when expression is limited only to the adult stages. No major effect is seen in male flies. Neuronal expression of parkScer\UAS.cGa does not reduce food consumption or increase physical activity or fecundity in young flies. Improved survival is seen following starvation.

Neuronal expression of parkScer\UAS.cGa under the control of Scer\GAL4elav.Switch.PO results in reduced levels of protein aggregates during ageing compared to controls.

The mitochondria of young and aged flies expressing parkScer\UAS.cGa under the control of Scer\GAL4da.Switch.PT show an increase in citrate synthase in young flies and mitochondrial respiratory complex I activity in aged flies.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4GMR.PF does not have any detectable effect on eye morphology.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference

Scer\GAL4da.PU/parkUAS.cGa is a suppressor of visible phenotype of Pink1B9

NOT Suppressor of
Other
Phenotype Manifest In
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference

Scer\GAL4da.PU/parkUAS.cGa is a suppressor of wing phenotype of Pink1B9

Scer\GAL4how-24B, Scer\GAL4how-24B, parkUAS.cGa is a suppressor | partially of mitochondrial crista & indirect flight muscle phenotype of Pink15

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The wing posture defects induced by the expression of Stoml2GD16706 under the control of Scer\GAL4fln.IFM alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, are fully suppressed by the co-expression of parkUAS.cGa.

The decreased adult climbing capacity observed upon the expression of Stoml2GD16706 under the control of Scer\GAL4ple.PU alone, or in combination with heterozygosity for either Df(2R)BSC695 or Df(2R)BSC770, is fully suppressed by the co-expression of parkUAS.cGa.

The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology (enlarged clustered mitochondria) and functional (loss of inner mitochondrial potential measured using cationic fluorescent dye JC-1) defects in adult indirect flight muscles characteristic for animals expressing cluGD13926 under the control of Scer\GAL4Mef2.PR can be suppressed by co-expression of parkScer\UAS.cGa.

The enlarged or aggregated mitochondria phenotype observed in the body wall muscles of Pink1B9 hemizygous male third instar larvae is suppressed either by combination with mask10.22/maskEY13048 alleles or by ectopic expression of parkScer\UAS.cGa under the control of Scer\GAL4how-24B.

Ubiquitous expression of parkScer\UAS.cGa under the control of Scer\GAL4da.PU restores climbing and flight ability in Pink1B9 mutant flies.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4da.PU rescues the reduction in ATP level seen in Pink1B9 mutant flies, but complex I activity is not significantly increased.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4ey-OK107 fails to suppress the axon degeneration seen in bskflp147E mutant mushroom body neuron clones.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4da.G32 significantly suppresses the penetrance of the indented thorax phenotype seen in Pink1B9 mutants. This suppression is reduced if the flies are also carrying either ref(2)Pod2 or ref(2)Pod3.

The ability of parkScer\UAS.cGa expressed under the control of Scer\GAL4da.G32 to suppress the Pink1B9 mutant phenotypes of an indented thorax and reduced climbing ability is suppressed if the flies are also mutant for Atg1Δ3D.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4da.PU results in lethality in the DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 double mutant background.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4da.PU rescues the wing posture defect and thoracic muscle apoptosis phenotype of Pink1B9/Pink1B9 mutants.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4Mef2.PR partially rescues the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink15 mutants.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4Mef2.PR fully rescues the thoracic indentation and flight phenotypes of Pink1B9 mutants.

The rough eye phenotype of flies expressing Pink1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is enhanced by co-expression of parkScer\UAS.cGa.

Co-expression of Pink1Scer\UAS.T:Ivir\HA1 and parkScer\UAS.cGa under the control of Scer\GAL4GMR.PF results in a severe rough eye phenotype, which greatly exceeds that conferred when Pink1Scer\UAS.T:Ivir\HA1 is expressed alone. This phenotype is not suppressed in a HtrA2Δ1 mutant background.

No eye phenotypes are observed when parkScer\UAS.cGa and HtrA2Scer\UAS.cPa are co-expressed under the control of Scer\GAL4GMR.PF.

Expression of parkScer\UAS.cGa enhances the rough eye phenotype seen when rho-7Scer\UAS.cWa is expressed under the control of Scer\GAL4GMR.PF, resulting in severe loss of eye tissue. This phenotype is not suppressed by HtrA2Δ1.

The indirect flight muscles of 2 day old Pink15 adults that are also expressing parkScer\UAS.cGa under the control of Scer\GAL4how-24B have some mitochondria with normal densely packed cristae and some with fragmented cristae, in contrast to 2 day old Pink15 single mutants, where the mitochondria of the indirect flight muscles have fragmented cristae.

Xenogenetic Interactions
Statement
Reference

Co-expression of parkScer\UAS.cGa rescues the age-dependent defect in climbing ability seen in flies expressing Rnor\Otcd.Scer\UAS under the control of Scer\GAL4da.G32. The defects in indirect flight muscle structure and mitochondrial integrity are also suppressed.

Co-expression of parkScer\UAS.cGa is no longer able to suppress the defects in climbing ability and indirect flight muscle structure seen in flies expressing Rnor\Otcd.Scer\UAS under the control of Scer\GAL4da.G32 when the flies are also mutant for Atg1Δ3D.

Complementation and Rescue Data
Comments

Expression of parkScer\UAS.cGa under the control of the Scer\GAL4how-24B driver rescues the defective mitochondria observed in body wall muscles of park25/parkΔ21 third instar larvae.

Expression of parkScer\UAS.cGa with a global (Scer\GAL4Act5C.PU) or neuronal (Scer\GAL4elav.PLu), but not muscle (Scer\GAL4G14), driver rescues locomotion in park25/parkZ3678 larvae; global and neuronal (but not muscle) expression partially rescues locomotion in park25/park25 larvae; neuronal but not muscle driver rescues contraction frequency in park25/park25 larvae; muscle expression rescues muscle resting membrane potential but not synaptic potential in park25/parkZ3678 larvae; neuronal expression partially rescues synaptic potential but not muscle resting membrane potential in park25/parkZ3678 larvae; global, neuronal and muscle drivers rescue synaptic overgrowth in park25/park25 larvae.

Expression of parkScer\UAS.cGa in the PPL neurons, under the regulation of Scer\GAL4ple.PF significantly attenuates dopaminergic (DA) neuron loss in the PPL cluster in park25 mutants.

The addition of parkScer\UAS.cGa driven by Scer\GAL4how-24B or Scer\GAL4Mef2.PR rescues the flight and climbing behaviour defects seen in park13/Df(3L)Pc-MK animals. parkScer\UAS.cGa driven by Scer\GAL4how-24B rescues the mitochondrial and myofibril defects seen in park25/Df(3L)Pc-MK indirect flight muscles.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
parkScer\UAS.cGa
parkUAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Greene
Secondary FlyBase IDs
    References (33)