FlyBase Allele Report: Dmel\DJ-1α[Δ72]

General Information

Symbol

Dmel\DJ-1α^Δ72

Species

D. melanogaster

Name

FlyBase ID

FBal0190611

Feature type

allele

Associated gene

Dmel\DJ-1α

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Meulener et al., 2005)

Mutagen

P-element activity

(Meulener et al., 2005)

Progenitor genotype

P{EPgy2}EY02281

(Meulener et al., 2005)

Cytology

Description

The entire coding region of DJ-1α is deleted.

(Meulener et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of early-onset Parkinson's disease

CEA

(West et al., 2015)

model of Parkinson's disease

CEA with dj-1β^Δ93

(Hao et al., 2010)

model of Parkinson's disease 7

CEC

(Himmelberg et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal visual perception

(West et al., 2015)

fertile

(Hao et al., 2010, Meulener et al., 2005)

short lived

(Faust et al., 2009)

viable

(Meulener et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

DJ-1α^Δ72 mutant flies show elevated response across a range of spatiotemporal frequencies of contrast-reversing sine-wave gratings as compared to responses from control flies.

(West et al., 2015)

No rhabdomere loss is observed in DJ-1α^Δ72 mutant flies.

(Sajjad et al., 2014)

DJ-1α^Δ72/DJ-1α^Δ72 mutants display normal eye development and ultrastructure.

(Aron et al., 2010)

DJ-1α^Δ72/DJ-1α^Δ72 mutants do not display any significant decrease in fertility.

(Hao et al., 2010)

DJ-1α^Δ72 mutant adults (reared on a yeast-paste diet) show significantly decreased survival rate compared to wild-type controls.

(Faust et al., 2009)

DJ-1α^Δ72 flies show no striking external phenotypes. These flies show no increase in sensitivity to agents that increase oxidative stress, or chemicals that induce the unfolded protein response or that impair proteasome function.

(Meulener et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

DJ-1α^Δ72 is a non-enhancer of chemical sensitive phenotype of dj-1β^Δ93

(Meulener et al., 2005)

Suppressor of

Statement

Reference

dj-1β^Δ93, dj-1beta[+], DJ-1alpha[+], DJ-1α^Δ72 is a suppressor of visible phenotype of Ras85D^G12V.sev, Scer\GAL4^GMR.PU

(Aron et al., 2010)

dj-1β^Δ93, dj-1beta[+], DJ-1alpha[+], DJ-1α^Δ72 is a suppressor of increased cell number | adult stage phenotype of Ras85D^G12V.sev

(Aron et al., 2010)

DJ-1alpha[+]/DJ-1α^Δ72, dj-1β^Δ93/dj-1β^Δ93 is a suppressor of visible phenotype of Ret^MEN2A.GMR

(Aron et al., 2010)

dj-1β^Δ93/dj-1beta[+], DJ-1α^Δ72/DJ-1α^Δ72 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

DJ-1alpha[+]/DJ-1α^Δ72 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

DJ-1α^Δ72/DJ-1α^Δ72 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

DJ-1alpha[+]/DJ-1α^Δ72 is a suppressor of increased cell size | adult stage phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

DJ-1α^Δ72/DJ-1α^Δ72 is a suppressor of increased cell size | adult stage phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

NOT Suppressor of

Statement

Reference

DJ-1alpha[+]/DJ-1α^Δ72 is a non-suppressor of visible phenotype of Pten^UAS.cKa, Scer\GAL4^GMR.PU

(Aron et al., 2010)

Other

Statement

Reference

DJ-1α^Δ72, Pink1^UAS.cUa, Scer\GAL4^da.PU, dj-1β^Δ93 has lethal phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has short lived phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has semi-sterile phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has abnormal locomotor behavior | progressive phenotype

(Hao et al., 2010)

DJ-1α^Δ72, Scer\GAL4^da.PU, dj-1β^Δ93, park^UAS.cGa has lethal phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has chemical sensitive phenotype

(Meulener, 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

DJ-1alpha[+]/DJ-1α^Δ72 is a suppressor of eye | adult stage phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

DJ-1α^Δ72/DJ-1α^Δ72 is a suppressor of eye | adult stage phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

DJ-1alpha[+]/DJ-1α^Δ72 is a suppressor of ommatidium | adult stage phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

DJ-1α^Δ72/DJ-1α^Δ72 is a suppressor of ommatidium | adult stage phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

dj-1β^Δ93, dj-1beta[+], DJ-1alpha[+], DJ-1α^Δ72 is a suppressor of eye phenotype of Ras85D^G12V.sev

(Aron et al., 2010)

dj-1β^Δ93, dj-1beta[+], DJ-1alpha[+], DJ-1α^Δ72 is a suppressor of eye photoreceptor cell | increased number | adult stage phenotype of Ras85D^G12V.sev

(Aron et al., 2010)

dj-1β^Δ93, dj-1beta[+], DJ-1alpha[+], DJ-1α^Δ72 is a suppressor of ommatidium phenotype of Ras85D^G12V.sev

(Aron et al., 2010)

DJ-1alpha[+]/DJ-1α^Δ72, dj-1β^Δ93/dj-1β^Δ93 is a suppressor of eye | adult stage phenotype of Ret^MEN2A.GMR

(Aron et al., 2010)

dj-1β^Δ93/dj-1beta[+], DJ-1α^Δ72/DJ-1α^Δ72 is a suppressor of eye | adult stage phenotype of Ret^MEN2B.GMR

(Aron et al., 2010)

NOT Suppressor of

Statement

Reference

DJ-1alpha[+]/DJ-1α^Δ72 is a non-suppressor of eye phenotype of Pten^UAS.cKa, Scer\GAL4^GMR.PU

(Aron et al., 2010)

Other

Statement

Reference

DJ-1α^Δ72, dj-1β^Δ93 has spermatozoon phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has major mitochondrial derivative phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has minor mitochondrial derivative phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has investment cone phenotype

(Hao et al., 2010)

DJ-1α^Δ72, dj-1β^Δ93 has mitochondrion | progressive phenotype

(Hao et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

DJ-1α^Δ72/+ dj-1β^Δ93/dj-1β^Δ93 significantly suppresses the small rough eye phenotype in Ret^MEN2A.GMR flies. DJ-1α^Δ72/+, DJ-1α^Δ72/DJ-1α^Δ72 or DJ-1α^Δ72/DJ-1α^Δ72 dj-1β^Δ93/+ significantly suppresses the small rough eye phenotype in Ret^MEN2B.GMR flies. DJ-1α^Δ72/+ or DJ-1α^Δ72/DJ-1α^Δ72 significantly suppresses the increase in ommatidia size in Ret^MEN2B.GMR flies. DJ-1α^Δ72/+ does not suppress the decreases in eye size seen in flies with expression of Pten^Scer\UAS.cKa driven by Scer\GAL4^GMR.PU. dj-1β^Δ93/+ DJ-1α^Δ72/+ suppresses eye phenotypes (including increase in R cell number per ommatidium) in Ras85D^G12V.sev flies.

(Aron et al., 2010)

DJ-1α^Δ72/DJ-1α^Δ72, dj-1β^Δ93/dj-1β^Δ93 double mutant flies have a reduced lifespan; display decreased spontaneous movement and climbing activity over time; have reduced fertility in both males and females; but have normal mitochondrial morphology, as compared with controls. Sperm from DJ-1α^Δ72/DJ-1α^Δ72, dj-1β^Δ93/dj-1β^Δ93 males appear morphologically normal but are immotile, have abnormal vacuoles in the Nebenkern, irregularities of mitochondrial derivatives, and misoriented and asynchronous progression of the investment cones during spermatogenesis. Mitochondria in DJ-1α^Δ72/DJ-1α^Δ72, dj-1β^Δ93/dj-1β^Δ93 double mutant flies show no difference in generating and maintaining cross-membrane potential, but do show a decline in mitochondrial function as the flies age, as measured by comparing the respiratory control ratio in 3 day- and 30 day-old whole flies.

Expression of park^Scer\UAS.cGa or Pink1^Scer\UAS.cUa under the control of Scer\GAL4^da.PU results in lethality in the DJ-1α^Δ72/DJ-1α^Δ72, dj-1β^Δ93/dj-1β^Δ93 double mutant background.

(Hao et al., 2010)

DJ-1α^Δ72; dj-1β^Δ93 flies are viable, fertile and have no striking external phenotypes. There are no differences in the number of DA neurons in the fly brain between young (1 day) or aged (30 days) flies. DJ-1α^Δ72; dj-1β^Δ93 flies show a higher sensitivity to the oxidative agents paraquat and H₂O₂ than wild-type flies. However, the sensitivity of these double mutants to paraquat is not significantly higher than in dj-1β^Δ93 single mutants. The double mutants do not show an increase in sensitivity to chemical agents that induce the unfolded protein response or that impair proteasome function. Expression of the either the dj-1β^Scer\UAS.cMa transgene or the DJ-1α^Scer\UAS.cMa transgene, under the control of Scer\GAL4^αTub84B.PL, rescues the sensitivity of dj-1β^Δ93; DJ-1α^Δ72 mutants to paraquat.

(Meulener et al., 2005)

DJ-1α^Δ72; dj-1β^Δ93 flies show a higher sensitivity to the oxidative agents paraquat, H₂O₂ and rotenone than wild-type flies.

(Meulener, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33600

w¹¹¹⁸; DJ-1α^Δ72

33602

w¹¹¹⁸; DJ-1α^Δ72; dj-1β^Δ93

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DJ-1A(Δ72)

(Faust et al., 2009)

DJ-1a[d72]

(Hao et al., 2010)

DJ-1α^Δ72

(Himmelberg et al., 2018, West et al., 2015, Sajjad et al., 2014)

d72

(Hao et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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