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General Information
Symbol
Dmel\parkHMS01800
Species
D. melanogaster
Name
FlyBase ID
FBal0278918
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
parkin RNAi, UAS-Park RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing parkHMS01800 under the control of Scer\GAL4Mef2.PR disrupts the mitochondrial network, with increased mitochondrial aggregates and increased number of mitolysosomes, in both adult and larval muscles, and wing posture defects, and wing posture defects.

Expression under the control of Scer\GAL4αTub84B.Switch.PK (induced by RU486 treatment) results in wing posture defects, decreased adult climbing activity and disorganization of muscle intestine fibers.

Expression under the control of Scer\GAL4elav.PLu leads to a decrease in the number of PPL1 neurons and a decrease in mitolysosomes in the larval and adult brain; there is decreased climbing activity.

The eyes of adults expressing parkHMS01800 under the control of Scer\GAL4GMR.PF show a progressive loss of rhabdomeres (more frequent at day 30 than at day 2).

Expression of parkHMS01800 under the control of Scer\GAL4how-24B results in deficit in larval locomotion: the crawling speed, distance from start to finish as well as the total length travelled over time are all significantly reduced in the knock-down third instar larvae compared to controls.

Expression of parkHMS01800 under the control of Scer\GAL4tin.CΔ4 evokes heart tube hypocontractility one and four weeks after eclosion, and increases cardiomyocyte mitochondrial size. Lifespan is similar to controls. The flies display an accelerated age-related decline in climbing performance.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of SodScer\UAS.cWa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01800 is expressed under the control of Scer\GAL4tin.CΔ4.

Expression of Sod2Scer\UAS.cMa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01800 is expressed under the control of Scer\GAL4tin.CΔ4.

Expression of CG6878KK108566 suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01800 is expressed under the control of Scer\GAL4tin.CΔ4.

Co-expression of MarfmiRNA.cUa.Scer\UAS and parkHMS01800 under the control of Scer\GAL4tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content seen in flies expressing parkHMS01800 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen with parkHMS01800, and appear fragmented as is seen with MarfmiRNA.cUa.Scer\UAS.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
parkHMS01800
Name Synonyms
Secondary FlyBase IDs
    References (17)