Open Close
General Information
Symbol
Dmel\MarfmiRNA.cUa.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0279441
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Marf RNAi, UAS-mfn RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a Marf RNAi construct.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 show a dilated cardiomyopathy characterised by mitochondria with fewer genomes, increased reactive oxygen species (ROS) and greater depolarization. There is no reduction in mitochondrial DNA.

Expression of MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel results in an approximately 30% reduction in eye area and results in ommatidial disorganisation.

Flies expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 show a progressive reduction in climbing activity compared to controls as they age. Heart tube fractional shortening is significantly depressed in the mutant flies at 4 weeks of age.

Expression of MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 increases mitochondrial heterogeneity, induces mitochondrial fragmentation in adult cardiomyocytes and shifts the population distribution towards smaller mitochondria.

Adults expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 have normal longevity.

Mitochondria tend to cluster in aggregates that distort the normal myofibrillar architecture in cardiomyocytes of adults expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4. The mitochondria appear heterogeneous, with both mitochondrial fragments and enlarged mitochondria being seen. Mean mitochondrial size is decreased approximately 30% compared to wild type. T-tubules and sarcoplasmic reticulum appears morphologically normal in the mutant cardiomyocytes.

The heart tubes of adults expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 are dilated compared to wild type and show impaired shortening with no change in the beating rate.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of MarfmiRNA.cUa.Scer\UAS and parkHMS01651 under the control of Scer\GAL4tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content usually seen in flies expressing parkHMS01651 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen in parkHMS01651, and appear fragmented as is seen with MarfmiRNA.cUa.Scer\UAS.

Co-expression of MarfmiRNA.cUa.Scer\UAS and parkHMS01800 under the control of Scer\GAL4tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content seen in flies expressing parkHMS01800 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen with parkHMS01800, and appear fragmented as is seen with MarfmiRNA.cUa.Scer\UAS.

The thoracic indentation phenotype of park25 Mul1A6 double mutants is almost completely suppressed by expression of MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4unspecified.

Co-expression of either MarfmiRNA.cUa.Scer\UAS or dmScer\UAS.cZa partially rescues the loss of type II neuroblasts seen in animals expressing NGD144 under the control of Scer\GAL4insc-Mz1407, while simultaneous co-expression of both MarfmiRNA.cUa.Scer\UAS and dmScer\UAS.cZa together fully rescues the phenotype.

The ability of rictorΔ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is blocked by co-expression of MarfmiRNA.cUa.Scer\UAS.

The ability of trcK122A.Scer\UAS.T:Zzzz\FLAG to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is blocked by co-expression of MarfmiRNA.cUa.Scer\UAS.

Co-expression of SodScer\UAS.cAa rescues the heart tube defects (dilation and impaired shortening) seen in adults expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4. In addition, the morphology of the mitochondria in the cardiomyocytes is markedly improved towards wild type.

Xenogenetic Interactions
Statement
Reference

Co-expression of MarfmiRNA.cUa.Scer\UAS results in a significant reduction of Pink1Scer\UAS.T:Ivir\HA1-mediated neuronal rescue in Scer\GAL4elav.PU>Hsap\HTTQ93.ex1p.Scer\UAS flies.

Co-expression of Hsap\MFN2Scer\UAS.cDa, but not of either Hsap\MFN2M393I.Scer\UAS or Hsap\MFN2R400Q.Scer\UAS, rescues the reduced eye area caused by expression of MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel.

The progressive reduction in climbing ability and depressed heart tube fractional shortening of flies expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 is almost completely rescued by co-expression of Hsap\MFN2Scer\UAS.cDa, but is not rescued by co-expression of either Hsap\MFN2M393I.Scer\UAS or Hsap\MFN2R400Q.Scer\UAS.

Mitochondrial fragmentation in the adult cardiomyocytes caused by expression of MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 is almost completely suppressed by co-expression of Hsap\MFN2Scer\UAS.cDa, but is not suppressed by co-expression of either Hsap\MFN2M393I.Scer\UAS or Hsap\MFN2R400Q.Scer\UAS.

Co-expression of Hsap\MFN2Scer\UAS.cDa completely rescues the heart tube defects (dilation and impaired shortening) seen in adults expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4.

Co-expression of Hsap\MFN1Scer\UAS.cDa can completely rescue the heart tube defects (dilation and impaired shortening) seen in adults expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4, depending on the Hsap\MFN1Scer\UAS.cDa line used.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
MarfmiRNA.cUa.Scer\UAS
MarfmiRNA.cUa.UAS
Name Synonyms
Secondary FlyBase IDs
    References (14)