FlyBase Allele Report: Scer\GAL4[αTub84B.Switch.PK]

General Information

Symbol

Scer\GAL4^{αTub84B.Switch.PK}

Species

S. cerevisiae

Name

FlyBase ID

FBal0244196

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{αTub84B-Switch.K}

Also Known As

tubGSGal4, tub5-GS-Gal4, TubGS, tub-GS-GAL4, tubulin-Gene-Switch, tub-GS, Tub5-GS, TubGS-Gal4, Tubulin-GS, tubulin-GeneSwitch-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Nielsen et al., 2008)

Progenitor genotype

Carried in construct

P{αTub84B-Switch.K}

Cytology

Description

αTub84B promoter sequences drive expression of the ligand-inducible GAL4::GS driver.

(Kabil et al., 2011)

Tubulin promoter sequences drive expression of Scer\GAL4.

(Nielsen et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

αTub84B

Encoded product / tool

GAL4::GS

binary expression system - small molecule-regulated driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage -- adult stage

organism | ubiquitous

(Rera et al., 2011)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression ubiquitously.

(FlyBase Curators, 2017)

ScerGAL4^{αTub84B.Switch.PK} drives mifepristone-inducible expression ubiquitously.

(Rera et al., 2011)

Marker for

Reflects expression of

αTub84B

(Rera et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal aging, with srl^UAS.cRa

(Rera et al., 2011)

abnormal developmental rate | RU486 conditional, with cype^GD908

(Kemppainen et al., 2014)

abnormal heat stress response | male limited | RU486 conditional, with Trap1^UAS.cBa

(Baqri et al., 2014)

abnormal locomotor behavior | adult stage, with park^HMS01800

(Gumeni et al., 2021)

abnormal locomotor behavior | adult stage, with Pink1^HMC04160

(Gumeni et al., 2021)

abnormal locomotor behavior | adult stage | RU486 conditional, with Hsap\MATR3^{F115C.UAS.Tag:FLAG}

(Ramesh et al., 2020)

abnormal locomotor behavior | adult stage | RU486 conditional, with Hsap\MATR3^{S85C.UAS.Tag:FLAG}

(Ramesh et al., 2020)

abnormal locomotor behavior | adult stage | RU486 conditional, with Hsap\MATR3^UAS.Tag:FLAG

(Ramesh et al., 2020)

abnormal mating behavior | RU486 conditional, with Akt^RNAi.UAS.cUa

(Kuo et al., 2012)

abnormal mating behavior | RU486 conditional, with InR^UAS.cHa

(Kuo et al., 2012)

abnormal mating behavior | RU486 conditional, with Pten^UAS.cUa

(Kuo et al., 2012)

abnormal memory | adult stage | RU486 conditional, with dib^RNAi.UAS.cOa

(Ishimoto et al., 2013)

abnormal memory | adult stage | RU486 conditional, with DopEcR^KK111211

(Ishimoto et al., 2013)

abnormal oxidative stress response, with cnc^RNAi.C.UAS

(Sykiotis and Bohmann, 2008)

abnormal oxidative stress response, with cnc^UAS.cSa

(Sykiotis and Bohmann, 2008)

abnormal oxidative stress response | adult stage | RU486 conditional, with Mrp4^EP3177

(Huang et al., 2014)

abnormal oxidative stress response | male limited | RU486 conditional, with Trap1^UAS.cBa

(Baqri et al., 2014)

abnormal oxidative stress response | RU486 conditional, with Atg6^UAS.Tag:HA

(Wu et al., 2009)

abnormal oxidative stress response | RU486 conditional, with Prx2^UASp.cDa

(Degennaro et al., 2011)

abnormal sleep | RU486 conditional, with hll^KK103275

(Thimgan et al., 2015)

abnormal starvation stress response | adult stage | RU486 conditional, with Sirt1^GD11580

(Banerjee et al., 2012)

abnormal stress response | RU486 conditional, with ND-20^GD13310

(Copeland et al., 2009)

abnormal stress response | RU486 conditional, with ND-SGDH^GD3876

(Copeland et al., 2009)

abnormal stress response | RU486 conditional, with UQCR-14L^GD9481

(Copeland et al., 2009)

abnormal wound healing, with bsk^DN.UAS

(Soares et al., 2014)

abnormal wound healing, with hep^CA.UAS

(Soares et al., 2014)

female semi-fertile | RU486 conditional, with ATPsynβL^GD11634

(Copeland et al., 2009)

female semi-fertile | RU486 conditional, with ND-20^GD13310

(Copeland et al., 2009)

female semi-fertile | RU486 conditional, with ND-SGDH^GD3876

(Copeland et al., 2009)

female semi-fertile | RU486 conditional, with UQCR-14L^GD9481

(Copeland et al., 2009)

lethal - all die before end of larval stage | RU486 conditional, with ND-20^GD13310

(Copeland et al., 2009)

lethal - all die before end of larval stage | RU486 conditional, with UQCR-14L^GD9481

(Copeland et al., 2009)

long lived | female | RU486 conditional, with ATPsynβL^GD11634

(Copeland et al., 2009)

long lived | female | RU486 conditional, with CG18809^GD9655

(Copeland et al., 2009)

long lived | female | RU486 conditional, with ND-20^GD13310

(Copeland et al., 2009)

long lived | female | RU486 conditional, with ND-SGDH^GD3876

(Copeland et al., 2009)

long lived | female | RU486 conditional, with Prp19^UAS.cGa

(Garschall et al., 2017)

long lived | female | RU486 conditional, with UQCR-14L^GD9481

(Copeland et al., 2009)

long lived | RU486 conditional, with Cbs^UAS.cKa

(Kabil et al., 2011)

long lived | RU486 conditional, with daw^HMS01110

(Bai et al., 2013)

long lived | RU486 conditional, with Diedel^RNAi.UAS

(Mlih et al., 2018)

long lived | RU486 conditional, with Glyp^HMS00032

(Bai et al., 2013)

long lived | RU486 conditional, with Gnmt^UAS.cOa

(Obata and Miura, 2015)

long lived | RU486 conditional, with InR^K1409A.UAS

(Obata and Miura, 2015)

long lived | RU486 conditional, with Mrp4^EP3177

(Huang et al., 2014)

long lived | RU486 conditional, with Smox^JF02320

(Bai et al., 2013)

long lived | RU486 conditional, with Tsp42Ef^GD3749

(Bai et al., 2013)

partially lethal - majority die | RU486 conditional, with COX5A^GD5167

(Kemppainen et al., 2014)

partially lethal - majority die | RU486 conditional, with COX5B^GD6659

(Kemppainen et al., 2014)

partially lethal - majority die | RU486 conditional, with cype^GD908

(Kemppainen et al., 2014)

partially lethal - majority die | RU486 conditional, with Surf1^RNAi.UAS

(Kemppainen et al., 2014)

short lived, with park^HMS01800

(Gumeni et al., 2021)

short lived, with Pink1^HMC04160

(Gumeni et al., 2021)

short lived, with srl^UAS.cRa

(Rera et al., 2011)

short lived | :calorie restriction conditional | RU486 conditional, with Cbs^KK109084

(Kabil et al., 2011)

short lived | calorie restriction conditional, with ATPsynβL^GD11634

(Bahadorani et al., 2010)

short lived | RU486 conditional, with Diedel^RNAi.UAS

(Mlih et al., 2018)

short lived | RU486 conditional, with Diedel^UAS.cMa

(Mlih et al., 2018)

short lived | RU486 conditional, with Hsap\MATR3^{F115C.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{F115C.ΔRRM1.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{F115C.ΔRRM2.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{S85C.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{S85C.ΔRRM1.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{S85C.ΔRRM2.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^UAS.Tag:FLAG

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{ΔRRM1.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{ΔRRM2.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{ΔZNF1.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Hsap\MATR3^{ΔZNF2.UAS.Tag:FLAG}

(Ramesh et al., 2020)

short lived | RU486 conditional, with Mad^JF01263

(Bai et al., 2013)

short lived | RU486 conditional, with Sams^KK107709

(Obata and Miura, 2015)

short lived | RU486 conditional, with Zzzz\CGG^90.UAS.EGFP

(Singh et al., 2021, Todd et al., 2010)

some die during larval stage | RU486 conditional, with ND-20^GD13310

(Copeland et al., 2009)

some die during larval stage | RU486 conditional, with UQCR-14L^GD9481

(Copeland et al., 2009)

some die during P-stage | RU486 conditional, with Lip3^UAS.cPa

(Bülow et al., 2018)

viable | RU486 conditional, with Lip3^UAS.cPa

(Bülow et al., 2018)

viable | RU486 conditional, with Sams^KK107709

(Obata and Miura, 2015)

visible | adult stage | RU486 conditional, with park^HMS01800

(Gumeni et al., 2021)

visible | adult stage | RU486 conditional, with Pink1^HMC04160

(Gumeni et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^{αTub84B.Switch.PK}, Zzzz\CGG^90.UAS.EGFP has short lived | male limited | RU486 conditional phenotype, enhanceable by kra^JF02556/Scer\GAL4^{αTub84B.Switch.PK}

(Singh et al., 2021)

NOT Enhanced by

Statement

Reference

Hsap\MATR3^UAS.Tag:FLAG, Scer\GAL4^{αTub84B.Switch.PK} has short lived | RU486 conditional phenotype, non-enhanceable by rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

Suppressed by

Statement

Reference

Pink1^HMC04160, Scer\GAL4^{αTub84B.Switch.PK} has visible | adult stage | RU486 conditional phenotype, suppressible by cnc^UAS.cSa, Scer\GAL4^{αTub84B.Switch.PK}

(Gumeni et al., 2021)

Scer\GAL4^{αTub84B.Switch.PK}, park^HMS01800 has visible | adult stage | RU486 conditional phenotype, suppressible by Scer\GAL4^{αTub84B.Switch.PK}/cnc^UAS.cSa

(Gumeni et al., 2021)

Scer\GAL4^{αTub84B.Switch.PK}, Zzzz\CGG^90.UAS.EGFP has short lived | RU486 conditional phenotype, suppressible by kra^UAS.Tag:FLAG/Scer\GAL4^{αTub84B.Switch.PK}

(Singh et al., 2021)

Hsap\MATR3^{F115C.UAS.Tag:FLAG}, Scer\GAL4^{αTub84B.Switch.PK} has short lived | RU486 conditional phenotype, suppressible | partially by rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

Hsap\MATR3^{S85C.UAS.Tag:FLAG}, Scer\GAL4^{αTub84B.Switch.PK} has short lived | RU486 conditional phenotype, suppressible | partially by rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

InR^K1409A.UAS, Scer\GAL4^{αTub84B.Switch.PK} has long lived | RU486 conditional phenotype, suppressible | partially by Gnmt^GD10563, Scer\GAL4^{αTub84B.Switch.PK}

(Obata and Miura, 2015)

Scer\GAL4^{αTub84B.Switch.PK}, Trap1^UAS.cBa has abnormal heat stress response | male limited | RU486 conditional phenotype, suppressible by ClpP^GD11514, Scer\GAL4^{αTub84B.Switch.PK}

(Baqri et al., 2014)

Scer\GAL4^{αTub84B.Switch.PK}, Trap1^UAS.cBa has abnormal heat stress response | male limited | RU486 conditional phenotype, suppressible by dve^KK115422/Scer\GAL4^{αTub84B.Switch.PK}

(Baqri et al., 2014)

Scer\GAL4^{αTub84B.Switch.PK}, cype^GD908 has abnormal developmental rate | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

Scer\GAL4^{αTub84B.Switch.PK}, Surf1^RNAi.UAS has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

COX5A^GD5167, Scer\GAL4^{αTub84B.Switch.PK} has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK/Zzzz\AOX[+]

(Kemppainen et al., 2014)

COX5A^GD5167, Scer\GAL4^{αTub84B.Switch.PK} has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK/Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

COX5B^GD6659, Scer\GAL4^{αTub84B.Switch.PK} has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK/Zzzz\AOX[+]

(Kemppainen et al., 2014)

COX5B^GD6659, Scer\GAL4^{αTub84B.Switch.PK} has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK/Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

Scer\GAL4^{αTub84B.Switch.PK}, cype^GD908 has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

DopEcR^KK111211, Scer\GAL4^{αTub84B.Switch.PK} has abnormal memory | adult stage | RU486 conditional phenotype, suppressible by Pde4^dnc-1

(Ishimoto et al., 2013)

NOT suppressed by

Statement

Reference

Hsap\MATR3^UAS.Tag:FLAG, Scer\GAL4^{αTub84B.Switch.PK} has short lived | RU486 conditional phenotype, non-suppressible by rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

Enhancer of

Statement

Reference

kra^JF02556/Scer\GAL4^{αTub84B.Switch.PK} is an enhancer of short lived | male limited | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, Zzzz\CGG^90.UAS.EGFP

(Singh et al., 2021)

NOT Enhancer of

Statement

Reference

rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK} is a non-enhancer of short lived | RU486 conditional phenotype of Hsap\MATR3^UAS.Tag:FLAG, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

Suppressor of

Statement

Reference

cnc^UAS.cSa, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of visible | adult stage | RU486 conditional phenotype of Pink1^HMC04160, Scer\GAL4^{αTub84B.Switch.PK}

(Gumeni et al., 2021)

Scer\GAL4^{αTub84B.Switch.PK}/cnc^UAS.cSa is a suppressor of visible | adult stage | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, park^HMS01800

(Gumeni et al., 2021)

kra^UAS.Tag:FLAG/Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of short lived | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, Zzzz\CGG^90.UAS.EGFP

(Singh et al., 2021)

rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor | partially of short lived | RU486 conditional phenotype of Hsap\MATR3^{F115C.UAS.Tag:FLAG}, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor | partially of short lived | RU486 conditional phenotype of Hsap\MATR3^{S85C.UAS.Tag:FLAG}, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

Gnmt^GD10563, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor | partially of long lived | RU486 conditional phenotype of InR^K1409A.UAS, Scer\GAL4^{αTub84B.Switch.PK}

(Obata and Miura, 2015)

ClpP^GD11514, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of abnormal heat stress response | male limited | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, Trap1^UAS.cBa

(Baqri et al., 2014)

dve^KK115422/Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of abnormal heat stress response | male limited | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, Trap1^UAS.cBa

(Baqri et al., 2014)

DopEcR^KK111211/Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of abnormal memory | adult stage phenotype of Pde4^dnc-2

(Ishimoto et al., 2013)

NOT Suppressor of

Statement

Reference

rump^KK102889, Scer\GAL4^{αTub84B.Switch.PK} is a non-suppressor of short lived | RU486 conditional phenotype of Hsap\MATR3^UAS.Tag:FLAG, Scer\GAL4^{αTub84B.Switch.PK}

(Ramesh et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pink1^HMC04160, Scer\GAL4^{αTub84B.Switch.PK} has wing | RU486 conditional phenotype, suppressible by cnc^UAS.cSa, Scer\GAL4^{αTub84B.Switch.PK}

(Gumeni et al., 2021)

Scer\GAL4^{αTub84B.Switch.PK}, park^HMS01800 has wing | RU486 conditional phenotype, suppressible by Scer\GAL4^{αTub84B.Switch.PK}/cnc^UAS.cSa

(Gumeni et al., 2021)

Enhancer of

Statement

Reference

ref(2)P^HMS00551/Scer\GAL4^{αTub84B.Switch.PK} is an enhancer of mitochondrion | adult stage phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

ref(2)P^HMS00551/Scer\GAL4^{αTub84B.Switch.PK} is an enhancer of adult somatic muscle cell phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

NOT Enhancer of

Statement

Reference

ref(2)P^HMS00551/Scer\GAL4^{αTub84B.Switch.PK} is a non-enhancer of mitochondrion | adult stage phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

ref(2)P^HMS00551/Scer\GAL4^{αTub84B.Switch.PK} is a non-enhancer of adult somatic muscle cell phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

Suppressor of

Statement

Reference

cnc^UAS.cSa, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of wing | RU486 conditional phenotype of Pink1^HMC04160, Scer\GAL4^{αTub84B.Switch.PK}

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of mitochondrion | adult stage phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of adult somatic muscle cell phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

Scer\GAL4^{αTub84B.Switch.PK}/cnc^UAS.cSa is a suppressor of wing | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, park^HMS01800

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of adult somatic muscle cell phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

DopEcR^UAS.cAa/Scer\GAL4^{αTub84B.Switch.PK} rescues DopEcR^c02142

(Aranda et al., 2017)

Scer\GAL4^{αTub84B.Switch.PK}/DCAF12^UAS.cHa rescues DCAF12^Δ1

(Hwangbo et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Gal4^GS-Tub

(Gumeni et al., 2019)

Gal4^Tub

(Tsakiri et al., 2021)

Scer\GAL4^{Mhc.Switch.PP}

Scer\GAL4^Switch.tub

Scer\GAL4^{αTub84B.Switch.PK}

Name Synonyms

Secondary FlyBase IDs

References (60)

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