FlyBase Allele Report: Dmel\Diedel[UAS.cMa]

General Information

Symbol

Dmel\Diedel^UAS.cMa

Species

D. melanogaster

Name

UAS construct of Mlih

FlyBase ID

FBal0344489

Feature type

allele

Associated gene

Dmel\Diedel

Associated Insertion(s)

Carried in Construct

P{UAS-Diedel.M}

Key Links

Transgenic product class

cDNA

(Mlih et al., 2018)

wild_type

(Mlih et al., 2018)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Mlih et al., 2018)

wild_type

(Mlih et al., 2018)

Mutagen

in vitro construct

(Mlih et al., 2018)

Progenitor genotype

Carried in construct

P{UAS-Diedel.M}

Cytology

Description

UASt regulates expression of an adult Drosophila Diedel cDNA.

(Mlih et al., 2018)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Diedel

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

decreased cell death | adult stage | progressive, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

decreased cell death | adult stage | progressive, with Scer\GAL4^Cg.PA

(Mlih et al., 2018)

decreased cell number | adult stage | somatic clone, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

decreased occurrence of cell division | adult stage, with Scer\GAL4^Cg.PA

(Mlih et al., 2018)

decreased occurrence of cell division | adult stage | conditional, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

decreased occurrence of cell division | adult stage | progressive, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

long lived | female limited, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

short lived, with Scer\GAL4^Cg.PA

(Mlih et al., 2018)

short lived | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Mlih et al., 2018)

Phenotype Manifest In

adult midgut epithelium | conditional, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

adult midgut epithelium | progressive, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

adult midgut epithelium | progressive, with Scer\GAL4^Cg.PA

(Mlih et al., 2018)

adult midgut epithelium | somatic clone, with Scer\GAL4^Act88F.1.3

(Mlih et al., 2018)

Detailed Description

Statement

Reference

Adults expressing Diedel^UAS.cMa under the control of Scer\GAL4^Act88F.1.3 or Scer\GAL4^Cg.PA do not show changes in feeding behavior compared to controls.

Scer\GAL4^Act88F.1.3-driven expression also results in: the adult midgut showing: significantly lower increases in mitosis during aging or in response to Ecc15 bacterial infection than in controls; the adult midgut not showing the age-dependent increase in apoptosis normally observed in controls; Ecc15-infected individuals showing a significantly higher bacterial burden in the midgut than similarly infected controls; adult midgut clones showing significantly fewer cells than clones in controls individuals.

Scer\GAL4^Cg.PA-driven expression also leads to the adult midgut not showing the age-dependent increase in apoptosis normally observed in controls.

Scer\GAL4^ppl.PP-driven expression does not significantly affect the apoptosis in the adult midgut (in 30-days old adults), as compared to controls.

Adulthood-only expression under the control of Scer\GAL4^{αTub84B.Switch.PK} and RU486 feeding leads to a RU486 concentration-dependent changes in in lifespan: 20mM increases; 50mM, 100mM or 200mM decrease.

(Mlih et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^sev.EP/Diedel^UAS.cMa is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Mlih and Karpac, 2022)

Scer\GAL4^sev.EP/Diedel^UAS.cMa is a non-suppressor of decreased size | adult stage phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa