FlyBase Allele Report: Dmel\DCAF12[Δ1]

General Information

Symbol

Dmel\DCAF12^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0319255

Feature type

allele

Associated gene

Dmel\DCAF12

Associated Insertion(s)

?{}DCAF12^Δ1

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Hwangbo et al., 2016)

Mutagen

Delta2-3 transposase

(Hwangbo et al., 2016)

Progenitor genotype

P{EPgy2}DCAF12^EY05707

(Hwangbo et al., 2016)

Associated Insertion(s)

?{}DCAF12^Δ1

Cytology

Description

1242bp deletion within DCAF12 (breakpoints 3R:12016888 and 3R:12015646 , release 6 sequence). In addition, TGATGAAAG has been inserted between the deletion breakpoints.

(Hwangbo and Jasper, 2016.6.30)

Deletion that removes the first three exons of DCAF12. Generated by imprecise excision of P{EPgy2}DCAF12^EY05707.

(Hwangbo et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:12,015,646..12,016,887 [-]

Comment:

A deletion of 1242 bp resulting from the imprecise excision of P{EPgy2}DCAF12^EY05707 extends from 3R:12016888 to 3R:12015646 . 9 bases of unidentified sequence (TGATGAAAG) are inserted at the site of the deletion.

(Hwangbo and Jasper, 2016.6.30)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | somatic clone

(Hwangbo et al., 2016)

decreased cell death | pupal stage | recessive

(Hwangbo et al., 2016)

increased cell number | P-stage

(Hwangbo et al., 2016)

lethal - all die during P-stage

(Hwangbo et al., 2016)

Phenotype Manifest In

cuticle | pharate adult stage

(Hwangbo et al., 2016)

embryonic/larval salivary gland

(Hwangbo et al., 2016)

eye | adult stage | somatic clone

(Hwangbo et al., 2016)

interommatidial bristle | pharate adult stage | increased number

(Hwangbo et al., 2016)

interommatidial cell | P-stage | increased number

(Hwangbo et al., 2016)

Detailed Description

Statement

Reference

DCAF12^Δ1/DCAF12^Δ1 clones in the eye outcompete heterozygous cells (over-growth, clone size larger than controls). DCAF12^Δ1/DCAF12^Δ1 mutant cells survive better than wild type cells and thus rescue growth in animals in which heterozygous or homozygous wild type cells are killed by expression of hid. DCAF12^Δ1/DCAF12^Δ1 eye discs in third instar larvae show similar patterns of mitotic cells to wild type. A small portion of DCAF12^Δ1/DCAF12^Δ1 mutant pupae survive to pharate adults and show lack of dorsal abdominal cuticle closure phenotypes, consistent with defects in the replacement of larval epidermal cells by histoblasts. Wild type larval salivary glands start to be degraded by apoptosis around 12 hours after puparium formation (APF) and by 16hr APF are completely histolyzed; this removal of larval salivary glands fails in DCAF12^Δ1/DCAF12^Δ1 mutants, with glands remaining intact until at least 48hr APF and lacking active caspase. Mutant brains and differentiating retina at 40-48hr APF show no gross differences compared to wild type. In wild type developing retina, apoptosis peaks at 32h APF; mutants have strong decreases in apoptosis at this time and excessive inter-ommatidial cells are present at 44hr APF, resulting in supernumerary bristles in the adult eye. Global activation of apoptosis during metamorphosis is lost in DCAF12^Δ1/DCAF12^Δ1 pupae, compared to heterozygotes.

(Hwangbo et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

DCAF12^Δ1 is an enhancer of neoplasia | adult stage | somatic clone phenotype of scrib¹

(Hwangbo et al., 2016)

Suppressor of

Statement

Reference

DCAF12^Δ1/DCAF12^Δ1 is a suppressor of cell lethal | somatic clone phenotype of pic^PL12c

(Hwangbo et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

DCAF12^Δ1 is an enhancer of eye | somatic clone phenotype of scrib¹

(Hwangbo et al., 2016)

Suppressor of

Statement

Reference

DCAF12^Δ1/DCAF12^Δ1 is a suppressor of eye | somatic clone phenotype of pic^PL12c

(Hwangbo et al., 2016)

DCAF12^Δ1/DCAF12^Δ1 is a suppressor of wing disc | somatic clone | third instar larval stage phenotype of pic^PL12c

(Hwangbo et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

scrib¹/scrib¹;DCAF12^Δ1/DCAF12^Δ1 double mutant clones show increased tissue overgrowth and scrib¹/scrib¹ mutant clones overexpressing DCAF12^Scer\UAS.cHa show enhanced tissue integrity (compared to scrib¹/scrib¹ clones).

DCAF12^Δ1/DCAF12^Δ1 suppresses the apoptotic phenotype (of limited clonal growth) in pic^PL12c/pic^PL12c eye clones or wing clones.

(Hwangbo et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

DCAF12^Δ1 is rescued by Scer\GAL4^{αTub84B.Switch.PK}/DCAF12^UAS.cHa

(Hwangbo et al., 2016)

Comments

Expression of DCAF12^Scer\UAS.cHa driven by Scer\GAL4^{αTub84B.Switch.PK} rescues pupal lethality in DCAF12^Δ1/DCAF12^Δ1 mutants.

(Hwangbo et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

DCAF12^Δ1

dcaf12^Δ1

(Hwangbo et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close