FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\DCAF12UAS.cHa
Open Close
General Information
Symbol
Dmel\DCAF12UAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hwangbo
FlyBase ID
FBal0319254
Feature type
allele
Associated gene
Dmel\DCAF12
Associated Insertion(s)
Carried in Construct
P{UAS-DCAF12.H}
Key Links
Transgenic product class
cDNA
(Hwangbo et al., 2016)
wild_type
(Hwangbo et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Hwangbo et al., 2016)
wild_type
(Hwangbo et al., 2016)
Mutagen
in vitro construct
(Hwangbo et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-DCAF12.H}
Cytology
Description

UASt regulatory sequences drive expression of a full-length DCAF12 cDNA which includes 5'UTR and 3'UTR sequences.

(Hwangbo et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
DCAF12
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of DCAF12Scer\UAS.cHa driven by Scer\GAL4da.PU leads to significant reductions in the size of male and female flies. Overexpression of DCAF12Scer\UAS.cHa limits clonal growth in the eyes (smaller clones as survival of mutant cells is decreased). Overexpression of DCAF12Scer\UAS.cHa driven in the developing wing disc by Scer\GAL4ptc.PU significantly reduces wing size between L3 and L4 in the adult and increases apoptosis in the third instar larval wing disc.

      Overexpression of DCAF12Scer\UAS.cHa in the developing retina driven by Scer\GAL4GMR.PU results in strong size reduction and disruption of normal patterning in the adult eye. Scer\GAL4GMR.PU>DCAF12Scer\UAS.cHa flies also show increased sensitivity of the retina to UV irradiation compared to controls: tissue loss in mutant retina in response to UV is much larger than controls.

      (Hwangbo et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Suppressor of
      Statement
      Reference

      DCAF12UAS.cHa is a suppressor of neoplasia | somatic clone | adult stage phenotype of scrib1

      (Hwangbo et al., 2016)
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      Statement
      Reference

      DCAF12UAS.cHa is a suppressor of eye | somatic clone phenotype of scrib1

      (Hwangbo et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of DroncCARD.Scer\UAS suppresses eye phenotypes in flies with expression of DCAF12Scer\UAS.cHa driven by Scer\GAL4GMR.PU. Diap1GMR.PH suppresses apoptotic eye phenotypes in flies with DCAF12Scer\UAS.cHa driven by Scer\GAL4GMR.PU.

      scrib1/scrib1;DCAF12Δ1/DCAF12Δ1 double mutant clones show increased tissue overgrowth and scrib1/scrib1 mutant clones overexpressing DCAF12Scer\UAS.cHa show enhanced tissue integrity (compared to scrib1/scrib1 clones).

      (Hwangbo et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of BacA\p35Scer\UAS.cUa suppresses the reduced size and disruption of adult eyes in flies with DCAF12Scer\UAS.cHa driven by Scer\GAL4GMR.PU.

      (Hwangbo et al., 2016)
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      DCAF12Scer\UAS.cHa
      DCAF12UAS.cHa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Hwangbo
      Secondary FlyBase IDs
        References (1)