Adulthood-specific expression of Mad^JF01263 under the combined control of Scer\GAL4^esg.PU and Scer\GAL80^Su(H).GBE (together with Gal80[ts] for the temporal regulation of expression) prevents re-establishment of quiescence (phospho-H3)in the adult gut upon Ecc15 bacterial infection, leading to dysplasia 2 days after infection; upon Pseudomonas entomophila infection, there is an increase in gut barrier dysfunction (in Smurf assays) and increased mortality, as compared to controls.

The adulthood-only expression of Mad^JF01263 under the control of Scer\GAL4^esg.PU (in combination with Gal80[ts], for temporal regulation of expression) leads to a significant decrease in the relative and absolute size of secondary cell nuclei compared to neighboring main cells and wild-type controls; this expression does not affect the size of main cell nuclei compared to controls. The adulthood-only expression under the control of Scer\GAL4^dsx.KI (and Gal80[ts]) leads to a significant decrease in the number of secretory vesicles within accessory gland secondary cells, associated with a significant decrease in the size of the largest multivesicular body/lysosome, as compared to controls; there is also a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen.

The adulthood-only expression of Mad^JF01263 under the combined control of Scer\GAL4^spi-NP0261 and Gal80[ts] also results in both virgin and mated males showing a significant increase in the number of dense core granule compartments within accessory gland secondary cells, as compared to matched controls.

Scer\GAL4^{αTub84B.Switch.PK} Mad^JF01263 adults, fed with RU486 to activate expression, show reduced lifespan compared to controls.