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General Information
Symbol
Dmel\hepCA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0098236
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hepCA, UAS-Hep.CA, HepCA, hepCA, UAS-HepCA, UAS-hemipterousCA, UAS-Hep
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a mutated form of hep (carries the amino acid replacements S346D, T350D and S352D).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

axon & dorsal cluster neuron, with Scer\GAL4ato.3.6

ventral adult lateral neuron & axon, with Scer\GAL4P2.4.Pdf

Detailed Description
Statement
Reference

40-hour- (but not 24-hour-) expression of hepCA.UAS under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) leads to occasional ectopic wings compared to controls.

Expression of hepCA.UAS under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

The expression of hepCA.Scer\UAS under the control of Scer\GAL4GMR.PU leads to a severe increase in apoptosis in the third instar larval eye disc morphogenetic furrow and to small eye and rough eye phenotypes in adults, as compared to controls; expression under the control of Scer\GAL4ptc-559.1 leads to a severe increase in apoptosis around the anterior-posterior compartment boundary of the third instar larval wing disc and to a highly penetrant anterior crossvein loss in the adult wing, as compared to controls.

Pulse (16 hr) expression of hepCA.Scer\UAS under the control of Scer\GAL4pnr-MD237, using tub-Gal80ts to limit the time of expression to third larval instar results in formation of a giant syncytium comprising the whole of Scer\GAL4pnr-MD237 expressing patches in the larval epidermis.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4GMR.PF induces cell death in the eye disc and generates a small-eye phenotype.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4wor.PA induces neuroblast loss in larval brains.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4sev.PU triggers extensive cell death in larval eye discs and produces rough eyes with reduced sizes.

Expression of constitutively active hepCA.Scer\UASunder the control of either Scer\GAL4elav.Switch.PO or Scer\GAL4αTub84B.Switch.PK results in a robust weakening of the regenerative response after axonal injury.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4elav.Switch.PO results in a marked increase in the size of pigmented scar after injury.

Expression of the constitutively active hepCA.Scer\UAS transgene, under the control of Scer\GAL4pnr-MD237, generates a small scutellum phenotype.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4ptc-559.1 results in loss of the anterior cross vein.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4GMR.PF results in reduced eclosion rates; 67% of flies eclose at 18[o]C and 0.75% at 24[o]C.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4GMR.PF results in a reduction in eye size; eye size is 17% of control in females and 18% in males.

Follicle cells expressing hepCA.Scer\UAS under the control of Scer\GAL4GR1 in unstarved flies display a hyper-engulfment phenotype in which they invade and surround apparently healthy nurse cells, culminating in the follicle cells engulfing intact nurse cells and inducing their death.

Expression of hepCA.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF induces JNK-mediated cell death and produces a small eye phenotype.

The eye-degeneration phenotype caused by expression of hepCA.Scer\UAS under the control of Scer\GAL4hs.2sev is not mitigated by feeding flies with linoleic acid and linolenic acid.

Expression of hepCA.Scer\UAS driven by Scer\GAL4sev.PU results in a rough eye phenotype. Large numbers of dead cells are detected in the eye imaginal disc of Scer\GAL4sev.PU>hepCA.Scer\UAS third instar larvae.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4GMR.PF produces a small eye phenotype.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4vg.PU results in lethality at 25oC, while at 18oC the flies are viable and have wing defects. The wing defects seen at 18oC include missing posterior wing-vein material, notches in the wing margins and held-out wings.

Weak expression of hepCA.Scer\UAS in the posterior region of the wing using the Scer\GAL4CY2 driver leads to a partial loss of the fifth longitudinal vein (L5).

Overexpression of hepCA.Scer\UAS in eye clones, under the control of Scer\GAL4GMR.PF results in a severe eye phenotype with apparently massive levels of apoptosis.

Expression of hepCA.Scer\UAS, under the control of Scer\GAL4ato.3.6, results in all dorsal cluster neurons crossing to the medulla, while in wild-type brains, the majority of these axons retract during pupal development and branch within the lobula.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4GMR.PF causes a reduction in eye size.

Expression of hepCA.Scer\UAS, under the control of Scer\GAL4P2.4.Pdf, induces axonal overextension of the sLNv without increased arborization.

Expression of hepCA.Scer\UAS, under the control of Scer\GAL4e22c, leads to embryonic lethality before dorsal closure.

When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is unaffected, lamellocyte numbers are increased and crystal cells are unaffected. Melanotic masses form. Similar, though milder, effects are seen when expression is driven by Scer\GAL4Hml.PG.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference

Scer\GAL4rn-GAL4-5, hepCA.UAS has visible | adult stage phenotype, non-enhanceable by CtBP[+]/CtBP87De-10

Suppressed by
Statement
Reference

Scer\GAL4GMR.PU, hepCA.UAS has visible | adult stage phenotype, suppressible | partially by Glyatc02982/Glyat[+]

NOT suppressed by
Statement
Reference

Scer\GAL4rn-GAL4-5, hepCA.UAS has visible | adult stage phenotype, non-suppressible by CtBP[+]/CtBP87De-10

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hepCA.UAS has visible phenotype, non-suppressible by Tab2[+]/Tab2G71

Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4vg.PU, hepCA.UAS has wing phenotype, enhanceable by flw1

NOT Enhanced by
Statement
Reference

Scer\GAL4rn-GAL4-5, hepCA.UAS has wing | ectopic phenotype, non-enhanceable by CtBP[+]/CtBP87De-10

Suppressed by
Statement
Reference

Scer\GAL4GMR.PU, hepCA.UAS has eye phenotype, suppressible | partially by Glyatc02982/Glyat[+]

NOT suppressed by
Statement
Reference

Scer\GAL4rn-GAL4-5, hepCA.UAS has wing | ectopic phenotype, non-suppressible by CtBP[+]/CtBP87De-10

Scer\GAL4sev.PU/Scer\GAL4sev.PU, hepCA.UAS has eye phenotype, non-suppressible by ben1

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hepCA.UAS has eye phenotype, non-suppressible by Tab2[+]/Tab2G71

Scer\GAL4P2.4.Pdf, hepCA.UAS has ventral adult lateral neuron & axon phenotype, non-suppressible by Appld

Suppressor of
Statement
Reference

hepCA.UAS/Scer\GAL4GR1 is a suppressor | partially of egg chamber | nutrition conditional phenotype of drprΔ5

hepCA.UAS/Scer\GAL4GR1 is a suppressor | partially of follicle cell | nutrition conditional phenotype of drprΔ5

hepCA.UAS/Scer\GAL4[-] is a suppressor | partially of embryo | dorsal closure stage phenotype of MtlΔ, Rac1J11, Rac2Δ

Additional Comments
Genetic Interactions
Statement
Reference

40-hour- (but not 24-hour-) expression of hepCA.UAS under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP87De-10/+ background leads to occasional ectopic wings compared to controls.

The increased apoptosis at the morphogenetic furrow of the third instar larval eye disc and the decreased eye size in adults resulting from the expression of hepCA.Scer\UAS under the control of Scer\GAL4GMR.PU are significantly suppressed by the co-expression of either GlyatTH00482 or bskNIG.5680R, or by heterozygosity for Glyatc02982; the increased apoptosis around the anterior-posterior compartment boundary of the third instar larval wing disc and the highly penetrant loss of the anterior crossvein in the adult wing resulting from the expression of hepCA.Scer\UAS under the control of Scer\GAL4ptc-559.1 are also partially suppressed by the co-expression of either GlyatTH00482 or bskNIG.5680R, or by heterozygosity for Glyatc02982.

The formation of giant syncytia in the patches of dorsal larval epidermis expressing hepCA.Scer\UAS under the control of Scer\GAL4pnr-MD237 (using tub-Gal80ts to limit the expression to 16 hr-long pulse during the third instar) is not suppressed by co-expression of either Cdk1GL00262, aurBGL00202 or DarkGD12048, the number of nuclei within the syncytia is also not changed.

ben1 has no effect on the rough eye phenotype seen when hepCA.Scer\UAS is expressed under the control of Scer\GAL4sev.PU.

Loss of Uev1A, through co-expression of Uev1AGD6650, fails to suppress the small scutellum phenotype found in flies expressing hepCA.Scer\UAS under the control of Scer\GAL4pnr-MD237.

Expression of hepCA.Scer\UAS under the control of Scer\GAL4GR1 partially rescues the cell death defects seen in drprΔ5 mutant starvation-induced degenerating egg chambers. Many abnormal egg chambers are still seen but, unlike in drprΔ5 mutants alone, enlargement of the follicle cells occurs and germline engulfment takes place. Hyper-engulfment, where follicle cells engulf intact nurse cells, takes place in some egg chambers.

A Df(3L)H99 background fails to suppress the small eye phenotype in flies expressing egrScer\UAS.cIa under the control of Scer\GAL4GMR.PF.

Expression of thScer\UAS.cHa fails to suppress the small eye phenotype in flies expressing egrScer\UAS.cIa under the control of Scer\GAL4GMR.PF.

Expression of NcC318G.Scer\UAS fails to suppress the small eye phenotype in flies expressing egrScer\UAS.cIa under the control of Scer\GAL4GMR.PF.

Co-expression of parkScer\UAS.T:Hsap\MYC strongly suppresses the rough eye phenotype in flies and the increased cell death in third instar larval eye discs seen with expression of hepCA.Scer\UAS driven by Scer\GAL4sev.PU.

Mkk4G587/Mkk4G673 does not suppress the small eye phenotype seen when hepCA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

Co-expression of Mkk4Scer\UAS.cGa suppresses the small eye phenotype seen when hepCA.Scer\UAS is expression of Scer\GAL4GMR.PF.

Co-expression of Mkk4Asp.Scer\UAS is unable to suppress the small eye phenotype seen when hepCA.Scer\UAS is expression of Scer\GAL4GMR.PF.

Co-expression of Mkk4Mut.Scer\UAS suppresses the small eye phenotype seen when hepCA.Scer\UAS is expression of Scer\GAL4GMR.PF.

The wing defects caused by expression of hepCA.Scer\UAS under the control of Scer\GAL4vg.PU are enhanced in a flw1/Y background; the wings are much smaller and are frequently notched and blistered. The double mutant flies are only 10% viable relative to their flw1/+ ; hepCA.Scer\UAS, Scer\GAL4vg.PU control siblings.

Co-expression of bskdsRNA.Scer\UAS with hepCA.Scer\UAS in eye clones, under the control of Scer\GAL4GMR.PF results in partial suppression of the apoptotic eye phenotype observed in hepCA.Scer\UAS mutant clones.

Overexpression of hepCA.Scer\UAS in shgk03401 mutant cephalic cells (i.e. cells in the larval brain and eye-antennal discs) expressing Ras85DV12.Scer\UAS results in neither enhanced tumour growth nor metastatic behaviour.

Expression of hepCA.Scer\UAS, under the control of Scer\GAL4P2.4.Pdf, in an Appld mutant background still induces the significant axonal extension seen when the transgene is expressed in a wild-type background.

Leaky expression of hepCA.Scer\UAS, with no GAL4 driver, partially rescues the lethality and dorsal puckering phenotype of Rac1J11 Rac2Δ MtlΔ triple mutants.

The dorsal open phenotype seen in homozygous Cka05836 embryos derived from females carrying homozygous Cka05836 germ-line clones is substantially rescued by expression of hepCA.Scer\UAS under the control of Scer\GAL4hs.PB using heat shock.

Xenogenetic Interactions
Statement
Reference

The formation of giant syncytia in the patches of dorsal larval epidermis expressing hepCA.Scer\UAS under the control of Scer\GAL4pnr-MD237 (using tub-Gal80ts to limit the expression to 16 hr-long pulse during the third instar) is not suppressed by co-expression of BacA\p35Scer\UAS.cUa, the number of nuclei within the syncytia is also not changed.

Expression of Zzzz\E4orf4Scer\UAS.cPa has little effect on the reduced eclosion rates seen when hepCA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF at 18[o]C. However at 24[o]C the reduced eclosion rate is partially suppressed, with 89% of flies eclosing (compared to 0.75% when hepCA.Scer\UAS is expressed alone).

Expression of Zzzz\E4orf4Scer\UAS.cPa partially rescues the eye phenotypes seen when hepCA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF at 18[o]C. Eye size is restored to 25% and 31% of controls in females and males respectively (from 17% and 18%).

Co-expression of Zzzz\lefScer\UAS.cGa with hepCA.Scer\UAS under the control of Scer\GAL4CY2 driver almost completely suppresses the hepCA.Scer\UAS partial loss of the fifth longitudinal vein (L5) phenotype, indicating that Zzzz\lef acts in parallel to or downstream of hep.

Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
hepCA.Scer\UAS
hepCA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (46)