FlyBase Allele Report: Dmel\aurB[GL00202]

General Information

Symbol

Dmel\aurB^GL00202

Species

D. melanogaster

Name

FlyBase ID

FBal0262675

Feature type

allele

Associated gene

Dmel\aurB

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00202}

Also Known As

GL00202, aurB RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00202}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00202

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

(Radford et al., 2015)

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

hypoplasia, with Scer\GAL4^mirr-DE

(Wang et al., 2015)

lethal, with Scer\GAL4^αTub84B.PL

(Jang et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of aurB^GL00202 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in chromosome condensation as well as microtubule assembly defects in mature oocytes.

(Nikalayevich and Ohkura, 2015)

Expression of aurB^GL00202 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} disrupts kinetochore assembly and complete loss of microtubules around the karyosome in oocytes.

Expression of aurB^GL00202 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in females results in sterility.

(Radford et al., 2015)

Expression of aurB^GL00202 under the control of Scer\GAL4^mirr-DE attenuates larval eye disc growth.

(Wang et al., 2015)

Females heterozygous for aurB^GL00202 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to aurB^GL00202 males results in: no eggs; egg/embryonic phenotype: no vitellogenic egg chambers; oogenesis defect.

(Sopko et al., 2014)

Expression of aurB^GL00202 under the control of Scer\GAL4^VP16.nos.UTR but not Scer\GAL4^{VP16.mat.αTub67C} leads to production of female germline cysts that do not develop past the germarium when compared to controls.

Expression of aurB^GL00202 under the control Scer\GAL4^{VP16.mat.αTub67C} leads to absence of microtubules surrounding the karyosome and a failure of homologous centromeres to separate (Scer\GAL4[VP16.mat.αTub67C]) in oocytes when compared to controls.

(Radford et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

aurB^GL00202/Scer\GAL4^pnr-MD237 is a non-suppressor of abnormal cell shape | third instar larval stage phenotype of Scer\GAL4^pnr-MD237, hep^CA.UAS

(Wang et al., 2015)

aurB^GL00202/Scer\GAL4^pnr-MD237 is a non-suppressor of increased cell size | third instar larval stage phenotype of Scer\GAL4^pnr-MD237, hep^CA.UAS

(Wang et al., 2015)

Other

Statement

Reference

pal¹/Df(2L)Exel6024, Scer\GAL4^{VP16.mat.αTub67C}, aurB^GL00202 has abnormal meiotic cell cycle | embryonic stage phenotype

(Dubruille et al., 2023)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, aurB^GL00202 has spindle microtubule | oogenesis phenotype, non-enhanceable by Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, aurB^GL00202 has spindle microtubule | oogenesis phenotype, non-suppressible by Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

NOT Enhancer of

Statement

Reference

aurB^GL00202, Scer\GAL4^{VP16.mat.αTub67C} is a non-enhancer of cytoplasmic microtubule | oogenesis phenotype of Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

Suppressor of

Statement

Reference

aurB^GL00202/Scer\GAL4^{VP16.mat.αTub67C} is a suppressor of male pronucleus | paternal effect phenotype of pal¹/Df(2L)Exel6024

(Dubruille et al., 2023)

NOT Suppressor of

Statement

Reference

aurB^GL00202/Scer\GAL4^pnr-MD237 is a non-suppressor of embryonic/larval epidermis | third instar larval stage phenotype of Scer\GAL4^pnr-MD237, hep^CA.UAS

(Wang et al., 2015)

aurB^GL00202, Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of cytoplasmic microtubule | oogenesis phenotype of Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of giant syncytia in the patches of dorsal larval epidermis expressing hep^CA.Scer\UAS under the control of Scer\GAL4^pnr-MD237 (using tub-Gal80ts to limit the expression to 16 hr-long pulse during the third instar) is not suppressed by co-expression of aurB^GL00202, the number of nuclei within the syncytia is also not changed.

(Wang et al., 2015)

Expression of aurB^GL00202 under the control of Scer\GAL4^{VP16.mat.αTub67C} modifies the overgrowing spindle microtubule phenotype of expression of Klp10A^HMS00920 under the control of Scer\GAL4^{VP16.mat.αTub67C}, and the resulting oocytes are absent of spindle microtubules.

(Radford et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

35299