FlyBase Allele Report: Dmel\Klp10A[HMS00920]

General Information

Symbol

Dmel\Klp10A^HMS00920

Species

D. melanogaster

Name

FlyBase ID

FBal0257593

Feature type

allele

Associated gene

Dmel\Klp10A

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00920}

Also Known As

Klp10A RNAi, HMS00920, klp10A RNAi #2

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00920}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00920

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cytoplasmic microtubule | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

meiotic spindle | female, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

meiotic spindle | female, with Scer\GAL4^{VP16.nanos.UTR}

(Radford et al., 2012)

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

spindle microtubule | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

Detailed Description

Statement

Reference

Expression of Klp10A^HMS00920 under the control of Scer\GAL4^ppk.PU, in combination with a Dicer-2 transgene to enhance RNAi efficiency, in larvae does not affect microtubule orientation in ddaC dendrites, compared to controls; there is no effect on pruning of ddaC dendrites by 16 h after puparium formation (APF), compared to controls.

(Wang et al., 2019)

Expression of Klp10A^HMS00920 under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to overgrowth of cytoplasmic and spindle microtubules in oocytes when compared to controls.

(Radford et al., 2012)

Oogenesis is completed and eggs are laid in females expressing Klp10A^HMS00920 under the control of either Scer\GAL4^{nos.UTR.T:Hsim\VP16} or Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, however the embryos fail to hatch. The mutant embryos show severe disorganisation of both the DNA and microtubules and no structures identifiable as pronuclear fusion or syncytial divisions are observed. Chromosomes are dispersed throughout the cytoplasm and microtubules form large asters surrounding the dispersed chromosomes.

Late-stage oocytes show defects in females expressing Klp10A^HMS00920 under the control of either Scer\GAL4^{nos.UTR.T:Hsim\VP16} or Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}. Firstly, spindle microtubule bundles are much longer than in wild type. Secondly, ooplasmic microtubules are more numerous and longer than in wild type and are often arranged in a starburst pattern. Spindle disorganisation ranging from slight (bipolar with some fraying or bending) to extreme (long microtubule bundles with no apparent poles) is seen. The central spindle region is often missing in the mutant oocytes, resulting in two disconnected half spindles and multipolar spindles. The karyosome and spindle are not in proper contact. Bi-orientation of homologous chromosomes is defective.

(Radford et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Klp10A^HMS00920, Scer\GAL4^en.PU is a suppressor of visible | adult stage phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal neuroanatomy | pupal stage phenotype of Patronin^UAS.cWa, Scer\GAL4^ppk.PU

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal cell shape | pupal stage phenotype of Patronin^UAS.cWa, Scer\GAL4^ppk.PU

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4⁴⁷⁷ is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Patronin^UAS.cWa, Scer\GAL4⁴⁷⁷

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4⁴⁷⁷ is a suppressor | partially of abnormal cell polarity | larval stage phenotype of Patronin^UAS.cWa, Scer\GAL4⁴⁷⁷

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal neuroanatomy | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal cell shape | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

NOT Suppressor of

Statement

Reference

Klp10A^HMS00920/Scer\GAL4^RapGAP1-OK6 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Patronin^EY05252/Df(2R)BSC355

(Gao et al., 2024)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C} has cytoplasmic microtubule | oogenesis phenotype, non-enhanceable by aurB^GL00202, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

NOT suppressed by

Statement

Reference

Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C} has cytoplasmic microtubule | oogenesis phenotype, non-suppressible by aurB^GL00202, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2012)

NOT Enhancer of

Statement

Reference

Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C} is a non-enhancer of spindle microtubule | oogenesis phenotype of Scer\GAL4^{VP16.mat.αTub67C}, aurB^GL00202

(Radford et al., 2012)

Suppressor of

Statement

Reference

Klp10A^HMS00920, Scer\GAL4^en.PU is a suppressor of wing phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Patronin^UAS.cWa, Scer\GAL4^ppk.PU

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of dendrite | pupal stage phenotype of Patronin^UAS.cWa, Scer\GAL4^ppk.PU

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4⁴⁷⁷ is a suppressor | partially of larval dorsal multidendritic neuron ddaC phenotype of Patronin^UAS.cWa, Scer\GAL4⁴⁷⁷

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4⁴⁷⁷ is a suppressor | partially of dendrite | larval stage phenotype of Patronin^UAS.cWa, Scer\GAL4⁴⁷⁷

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4⁴⁷⁷ is a suppressor | partially of microtubule | larval stage phenotype of Patronin^UAS.cWa, Scer\GAL4⁴⁷⁷

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a suppressor | partially of dendrite | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

NOT Suppressor of

Statement

Reference

Klp10A^HMS00920/Scer\GAL4^RapGAP1-OK6 is a non-suppressor of NMJ bouton | larval stage phenotype of Patronin^EY05252/Df(2R)BSC355

(Gao et al., 2024)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a non-suppressor of dendritic microtubule phenotype of Scer\GAL4^ppk.PU, chb^GL00367

(Bu et al., 2022)

Klp10A^HMS00920, Scer\GAL4^ppk.PU is a non-suppressor of larval dorsal multidendritic neuron ddaC phenotype of Scer\GAL4^ppk.PU, chb^GL00367

(Bu et al., 2022)

Klp10A^HMS00920, Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of spindle microtubule | oogenesis phenotype of Scer\GAL4^{VP16.mat.αTub67C}, aurB^GL00202

(Radford et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of aurB^GL00202 under the control of Scer\GAL4^{VP16.mat.αTub67C} modifies the overgrowing spindle microtubule phenotype of expression of Klp10A^HMS00920 under the control of Scer\GAL4^{VP16.mat.αTub67C}, and the resulting oocytes are absent of spindle microtubules.

(Radford et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33963