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General Information
Symbol
Dmel\srlUAS.cRa
Species
D. melanogaster
Name
FlyBase ID
FBal0280490
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of srl coding sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32 is sufficient to increase mitochondrial biogenesis and bioenergetic efficiency.

Expression of SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32 does not produce any gross changes in body size or obvious differences in size, morphology, or cell number of external structures, such as wings.

There is an approximate 20% reduction in triglyceride (TAG) metabolism in flies expressing SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32. In contrast, there is a significant increase in both the amount of stored glycogen and free glucose levels.

Ubiquitous expression of SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32 results in a moderate decrease in adult survival. Similar effects are observed upon expression of SpargelScer\UAS.cRa under the control of Scer\GAL4αTub84B.Switch.PK.

Life-span is significantly increased when SpargelScer\UAS.cRa is induced by Scer\GAL4Switch1.106, which is expressed in abdominal fat and the digestive tract. Induction of SpargelScer\UAS.cRa in the digestive tract (but not the fat body) under the control of Scer\GAL4TIGS-2 results in a 33% increase in mean life span and a 37% increase in maximum life span in female flies, with no major effect in male flies. Notably, adult survival is not improved when SpargelScer\UAS.cRa is induced with the pan-neuronal driver Scer\GAL4elav.Switch.PO.

Expression of SpargelScer\UAS.cRa in the digestive tract under the control of Scer\GAL4esg-05730B generates a 49% increase in mean survival. However, food consumption is not affected.

Scer\GAL4esg-05730B-mediated expression of SpargelScer\UAS.cRa is sufficient to confer a decrease in triglyceride (TAG) levels. Although Scer\GAL4esg-05730B>SpargelScer\UAS.cRa flies display normal glycogen stores, restricted expression of SpargelScer\UAS.cRa is sufficient to produce a moderate increase in free glucose levels. Long-lived Scer\GAL4esg-05730B>SpargelScer\UAS.cRa flies display normal fertility compared to age-matched controls. There is no difference in survival time for Scer\GAL4esg-05730B>SpargelScer\UAS.cRa under conditions of starvation and oxidative stress, compared to wild-type. In addition, these long-lived flies display only a marginal increase in survival under hyperoxia.

The intestines of long-lived Scer\GAL4esg-05730B>SpargelScer\UAS.cRa flies display significant maintenance of mitochondrial membrane potential, compared to controls, where membrane potential is progressively lost. Targeted expression of SpargelScer\UAS.cRa in intestinal stem cells and enteroblasts is sufficient to lower the levels of reactive oxygen species throughout the intestinal epithelium in aged flies.

Expression of SpargelScer\UAS.cRa in the digestive tract (under the control of Scer\GAL4bun-GSG5961) results in a significant increase in life span. Adult-only induction of SpargelScer\UAS.cRa (through RU486 addition to the food) results in a significant increase in life span.

An increase in SpargelScer\UAS.cRa expression in the digestive tract under the control of Scer\GAL4esg-05730B results in improved intestinal integrity in aged flies, which is consistent with a delay in disruption of apical-basal polarity in intestines from ageing flies, as revealed by arm staining.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of LamCUAS.cSa and srlUAS.cRa under the control of Scer\GAL4fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
SpargelScer\UAS.cRa
srlScer\UAS.cRa
srlUAS.cRa
Name Synonyms
Secondary FlyBase IDs
    References (6)