FB2026_02 , released June 18, 2026
Allele: Dmel\srlUAS.cRa
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General Information
Symbol
Dmel\srlUAS.cRa
Species
D. melanogaster
Name
FlyBase ID
FBal0280490
Feature type
allele
Associated gene
Dmel\srl
Associated Insertion(s)
Carried in Construct
P{UAS-Spargel.R}
Also Known As
uas-srl
Key Links
Transgenic product class
wild_type
(Rera et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Rera et al., 2011)
Mutagen
in vitro construct
(Rera et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Spargel.R}
Cytology
Description

UAS regulatory sequences drive expression of srl coding sequences.

(Rera et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
srl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  congenital muscular dystrophy due to LMNA mutation
      modeled by LamCG489V.UAS
      (Chandran et al., 2019)
      ameliorates  Barth syndrome
      modeled by Taz889
      (Damschroder et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32 is sufficient to increase mitochondrial biogenesis and bioenergetic efficiency.

      Expression of SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32 does not produce any gross changes in body size or obvious differences in size, morphology, or cell number of external structures, such as wings.

      There is an approximate 20% reduction in triglyceride (TAG) metabolism in flies expressing SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32. In contrast, there is a significant increase in both the amount of stored glycogen and free glucose levels.

      Ubiquitous expression of SpargelScer\UAS.cRa under the control of Scer\GAL4da.G32 results in a moderate decrease in adult survival. Similar effects are observed upon expression of SpargelScer\UAS.cRa under the control of Scer\GAL4αTub84B.Switch.PK.

      Life-span is significantly increased when SpargelScer\UAS.cRa is induced by Scer\GAL4Switch1.106, which is expressed in abdominal fat and the digestive tract. Induction of SpargelScer\UAS.cRa in the digestive tract (but not the fat body) under the control of Scer\GAL4TIGS-2 results in a 33% increase in mean life span and a 37% increase in maximum life span in female flies, with no major effect in male flies. Notably, adult survival is not improved when SpargelScer\UAS.cRa is induced with the pan-neuronal driver Scer\GAL4elav.Switch.PO.

      Expression of SpargelScer\UAS.cRa in the digestive tract under the control of Scer\GAL4esg-05730B generates a 49% increase in mean survival. However, food consumption is not affected.

      Scer\GAL4esg-05730B-mediated expression of SpargelScer\UAS.cRa is sufficient to confer a decrease in triglyceride (TAG) levels. Although Scer\GAL4esg-05730B>SpargelScer\UAS.cRa flies display normal glycogen stores, restricted expression of SpargelScer\UAS.cRa is sufficient to produce a moderate increase in free glucose levels. Long-lived Scer\GAL4esg-05730B>SpargelScer\UAS.cRa flies display normal fertility compared to age-matched controls. There is no difference in survival time for Scer\GAL4esg-05730B>SpargelScer\UAS.cRa under conditions of starvation and oxidative stress, compared to wild-type. In addition, these long-lived flies display only a marginal increase in survival under hyperoxia.

      The intestines of long-lived Scer\GAL4esg-05730B>SpargelScer\UAS.cRa flies display significant maintenance of mitochondrial membrane potential, compared to controls, where membrane potential is progressively lost. Targeted expression of SpargelScer\UAS.cRa in intestinal stem cells and enteroblasts is sufficient to lower the levels of reactive oxygen species throughout the intestinal epithelium in aged flies.

      Expression of SpargelScer\UAS.cRa in the digestive tract (under the control of Scer\GAL4bun-GSG5961) results in a significant increase in life span. Adult-only induction of SpargelScer\UAS.cRa (through RU486 addition to the food) results in a significant increase in life span.

      An increase in SpargelScer\UAS.cRa expression in the digestive tract under the control of Scer\GAL4esg-05730B results in improved intestinal integrity in aged flies, which is consistent with a delay in disruption of apical-basal polarity in intestines from ageing flies, as revealed by arm staining.

      (Rera et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1HMS01707, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Pink1HMS01707, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa/Scer\GAL4ple.PF is a suppressor of decreased cell number | adult stage | progressive phenotype of Scer\GAL4ple.PF, parkHMS01651

      (Pirooznia et al., 2020)

      srlUAS.cRa/Scer\GAL4ple.PF is a suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4ple.PF, parkHMS01651

      (Pirooznia et al., 2020)

      srlUAS.cRa/Scer\GAL4ple.PF is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4ple.PF, parkHMS01651

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of decreased cell number | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of decreased cell number | adult stage | progressive phenotype of Pink1HMS01707, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4fln.IFM is a suppressor | partially of flightless | progressive phenotype of LamCG489V.UAS, Scer\GAL4fln.IFM

      (Chandran et al., 2019)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      srlUAS.cRa/Scer\GAL4ple.PF is a suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Scer\GAL4ple.PF, parkHMS01651

      (Pirooznia et al., 2020)

      srlUAS.cRa/Scer\GAL4ple.PF is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4ple.PF, parkHMS01651

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of mitochondrion | adult stage phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Pink1HMS01707, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4ple.PF is a suppressor of mitochondrion | adult stage phenotype of Pink1HMS01707, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      srlUAS.cRa, Scer\GAL4fln.IFM is a suppressor | partially of indirect flight muscle cell | adult stage phenotype of LamCR205W.UAS, Scer\GAL4fln.IFM

      (Chandran et al., 2019)

      srlUAS.cRa, Scer\GAL4fln.IFM is a suppressor of indirect flight muscle cell | adult stage phenotype of LamCG489V.UAS, Scer\GAL4fln.IFM

      (Chandran et al., 2019)

      srlUAS.cRa, Scer\GAL4fln.IFM is a suppressor of wing phenotype of LamCG489V.UAS, Scer\GAL4fln.IFM

      (Chandran et al., 2019)

      srlUAS.cRa, Scer\GAL4fln.IFM is a suppressor of nucleus | adult stage phenotype of LamCG489V.UAS, Scer\GAL4fln.IFM

      (Chandran et al., 2019)

      srlUAS.cRa, Scer\GAL4fln.IFM is a suppressor of myofibril | adult stage phenotype of LamCG489V.UAS, Scer\GAL4fln.IFM

      (Chandran et al., 2019)

      srlUAS.cRa, Scer\GAL4fln.IFM is a suppressor of mitochondrion | adult stage phenotype of LamCG489V.UAS, Scer\GAL4fln.IFM

      (Chandran et al., 2019)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of LamCUAS.cSa and srlUAS.cRa under the control of Scer\GAL4fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

      (Chandran et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      PGC-1JW
      (Diop et al., 2015)
      SpargelScer\UAS.cRa
      srlScer\UAS.cRa
      srlUAS.cRa
      Name Synonyms
      Secondary FlyBase IDs
        References (8)