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General Information
Symbol
Dmel\parkHMS01651
Species
D. melanogaster
Name
FlyBase ID
FBal0266558
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Parkin RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adults expressing parkHMS01651 under the control of Scer\GAL4Act5C.PI or Scer\GAL4ple.PF show a progressive decline in climbing activity, and a progressive decrease in DA neuron number (PPL1, PPL2, PPM1/2 and PPM3 clusters); DA neurons also show decreased abundance of mitochondria.

The expression of parkHMS01651 under the control of Scer\GAL4Act.PU in clones within mosaic midguts results in enterocytes during pupariation, but not after puparium formation, exhibiting a significant increase in the transverse cell area, a significant increase in the relative mitochondrial content and a significant decrease in the relative amount of autophagosomes, as assessed by a Atg8a fluorescent reporter, as compared to non-clone enterocytes.

Expression of parkHMS01651 under the control of Scer\GAL4tin.CΔ4 evokes heart tube hypocontractility at one and four weeks after eclosion, and increases cardiomyocyte mitochondrial size. The lifespan of these flies is similar to controls but the flies display an accelerated age-related decline in climbing performance. Heart tube end-systolic and end-diastolic dimensions are increased and mitochondria have decreased DNA, increase mitochondrial reactive oxygen species (ROS) and increased numbers of depolarized organelles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of SodScer\UAS.cWa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.

Expression of Sod2Scer\UAS.cMa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.

Expression of CG6878KK108566 suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.

Co-expression of MarfmiRNA.cUa.Scer\UAS and parkHMS01651 under the control of Scer\GAL4tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content usually seen in flies expressing parkHMS01651 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen in parkHMS01651, and appear fragmented as is seen with MarfmiRNA.cUa.Scer\UAS.

Xenogenetic Interactions
Statement
Reference

Co-expression of parkHMS01651 partially suppresses eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies. Co-expression of parkHMS01651 does not suppress the protective role of expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 in suppression of eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
parkHMS01651
Name Synonyms
Secondary FlyBase IDs
    References (8)