FlyBase Allele Report: Hsap\SNCA[UAS.cJa]

General Information

Symbol

Hsap\SNCA^UAS.cJa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Jackson

FlyBase ID

FBal0287844

Feature type

allele

Associated gene

Hsap\SNCA

Associated Insertion(s)

Carried in Construct

P{UAS-SNCA.J}

Also Known As

UAS-SNCA, UAS-α-synuclein, UAS-αSyn, UAS-alpha-synuclein

Key Links

Transgenic product class

wild_type

(Jackson, 2013.9.11)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Jackson, 2013.9.11)

Mutagen

in vitro construct

(Jackson, 2013.9.11)

Progenitor genotype

Carried in construct

P{UAS-SNCA.J}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\SNCA. This construct was generated using the P{Express-UAS} vector.

(Jackson, 2013.9.11)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\SNCA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 1

CEA

(Moore et al., 2025, Spargo et al., 2025, Matsuoka et al., 2024, Yu et al., 2023, Dabool et al., 2020, Wu et al., 2019, Rousseaux et al., 2018, Chouhan et al., 2016)

CEC

(Ren et al., 2022)

model of Parkinson's disease

CEC

(Jansen et al., 2017)

CEA with park^HMS01651

(Narwal et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease 1

is ameliorated by Ca-α1D^MI08906

(Rousseaux et al., 2018)

is ameliorated by Ddc^ts2

(Rousseaux et al., 2018)

is ameliorated by Egfr^{FRT.RNAi.slbo}

(Rousseaux et al., 2018)

is ameliorated by Eip63E^{MI00413-GFSTF.1}

(Rousseaux et al., 2018)

is ameliorated by Eip63E^MI04853

(Rousseaux et al., 2018)

is ameliorated by Loxl2^MI06311

(Rousseaux et al., 2018)

is ameliorated by N^fa-swb

(Rousseaux et al., 2018)

is ameliorated by bgm^MI14249

(Rousseaux et al., 2018)

is ameliorated by cac^GD15341

(Rousseaux et al., 2018)

is exacerbated by ABCB7^03-232

(Ren et al., 2022)

is exacerbated by CG4553^06-125

(Ren et al., 2022)

is exacerbated by Cdc27^02-055

(Ren et al., 2022)

is exacerbated by Cdk8^GL00231

(Ren et al., 2022)

is exacerbated by CycC^HMS01095

(Ren et al., 2022)

is exacerbated by Eip75B^12-111

(Ren et al., 2022)

is exacerbated by GlyRS^08-107

(Ren et al., 2022)

is ameliorated by Hph^KK100344

(Ren et al., 2022)

is exacerbated by MTA1-like^06-040

(Ren et al., 2022)

is exacerbated by ND-39^11-101

(Ren et al., 2022)

is ameliorated by Vhl^KK111257

(Ren et al., 2022)

is exacerbated by cpo^10-129

(Ren et al., 2022)

is exacerbated by dlt^05-188

(Ren et al., 2022)

is exacerbated by heca^06-232

(Ren et al., 2022)

is exacerbated by kto^HMS06027

(Ren et al., 2022)

is exacerbated by l(3)87Df^10-122

(Ren et al., 2022)

is exacerbated by sima^KK102226

(Ren et al., 2022)

is exacerbated by skd^06-056

(Ren et al., 2022)

is exacerbated by skd^HMS01305

(Ren et al., 2022)

is exacerbated by skd^MI12229

(Ren et al., 2022)

is exacerbated by skd^T606

(Ren et al., 2022)

is exacerbated by Spn27A^KK109962

(Moore et al., 2025)

is exacerbated by Spn31A^GD5369

(Moore et al., 2025)

is exacerbated by Spn31A^KK106055

(Moore et al., 2025)

is exacerbated by Spn42Da^KK107708

(Moore et al., 2025)

is exacerbated by Spn42De^GD14221

(Moore et al., 2025)

is exacerbated by Spn42De^KK103216

(Moore et al., 2025)

is exacerbated by Spn47C^GD9948

(Moore et al., 2025)

is ameliorated by SkpA^UAS.ORF

(Dabool et al., 2020)

is exacerbated by Dap160^Δ1

(Spargo et al., 2025)

is ameliorated by Dap160^UAS.cKa

(Spargo et al., 2025)

is exacerbated by Csp^R1

(Yu et al., 2023)

is exacerbated by Ect3^KK102324

(Yu et al., 2023)

is exacerbated by Ect3^NIG.3132R

(Yu et al., 2023)

is exacerbated by Gba1b^GD10253

(Yu et al., 2023)

is exacerbated by Gba1b^KK107189

(Yu et al., 2023)

is exacerbated by Lip4^GD5503

(Yu et al., 2023)

is exacerbated by Lip4^KK106457

(Yu et al., 2023)

is exacerbated by Npc1a¹

(Yu et al., 2023)

is exacerbated by Npc1a²

(Yu et al., 2023)

is exacerbated by Npc1a^KK101077

(Yu et al., 2023)

is exacerbated by Snmp1^GD478

(Yu et al., 2023)

model of Parkinson's disease

is exacerbated by CG32191^KK103889

(Jansen et al., 2017)

is exacerbated by CG12025^KK107653

(Jansen et al., 2017)

is exacerbated by CG12025^GD2253

(Jansen et al., 2017)

is exacerbated by Ptp10D^GD115

(Jansen et al., 2017)

is exacerbated by Ptp10D^KK101775

(Jansen et al., 2017)

is exacerbated by CG32191^GD6163

(Jansen et al., 2017)

is exacerbated by Ptp10D^GD2611

(Jansen et al., 2017)

is exacerbated by Vps13^HMS01715

(Jansen et al., 2017)

is exacerbated by Vps13^HMS02460

(Jansen et al., 2017)

exacerbates developmental and epileptic encephalopathy 4

modeled by Rop^A3

(Matsuoka et al., 2024)

modeled by Rop^G27

(Matsuoka et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Jansen et al., 2017, Chouhan et al., 2016)

abnormal neurophysiology | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

Phenotype Manifest In

eye photoreceptor cell | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

nerve terminal | adult stage, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

ommatidium | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

retina | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

rhabdomere of eye photoreceptor cell | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

Detailed Description

Statement

Reference

Expression of Hsap\SNCA^Scer\UAS.cJa under the control of Scer\GAL4^ninaE.PT results in age-progressive degeneration of ommatidia as well as vacuolar lesions in the adult retina.

(Jansen et al., 2017)

Expression of Hsap\SNCA^Scer\UAS.cJa driven by Scer\GAL4^ninaE.PT (crosses carried out at 18[o]C and shifted to 25[o]C following eclosion) does not significantly affect external eye morphology in adult flies. At 30 days old (but not 1 or 10), electroretinogram (ERG) recordings of light induced potentials from Scer\GAL4^ninaE.PT>Hsap\SNCA^Scer\UAS.cJa flies show a significant decrease in light-evoked in depolarization amplitude compared to controls; mutant flies show significant increases in ON transient amplitude at day 10 and 30 and significant decreases in OFF transient amplitude at day 10 and 30 (no differences at day 1).

The number and morphology of photoreceptors (retinae show intact ommatidia with 7 visible rhabdomeres) in Scer\GAL4^ninaE.PT>Hsap\SNCA^Scer\UAS.cJa flies is grossly preserved at day 1, similar to controls; with aging (day 10 and 30), progressive vacuolar changes affect the retina and there is a significant decrease in rhabdomere numbers at day 10 (at day 30 the severity of tissue loss and degeneration is such that ommatidia cannot be reliably identified). Photoreceptor ultrastructure (examined with TEM) in 10 day old Scer\GAL4^ninaE.PT>Hsap\SNCA^Scer\UAS.cJa flies revealed significant subtle defects and irregularities in rhabdomeres (splitting or fraying at edges, vacuolar changes). Mutants at day 10 have similar numbers of mitochondria per ommatidium and percentage of photoreceptors with electron dense vacuoles (telolysosomes) as controls, but have a significant increase in the number of autophagic vacuoles (increased numbers of multivesicular or multilamellar bodies). Scer\GAL4^ninaE.PT>Hsap\SNCA^Scer\UAS.cJa flies show significant decrease in photoreceptor synaptic terminal area and synaptic vesicle density at 10 days old compared to controls.

(Chouhan et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by CG12025^GD2253, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Ptp10D^KK101775, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Ptp10D^GD115, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by CG32191^GD6163, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Ptp10D^GD2611, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Vps13^HMS01715, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Vps13^HMS02460, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Ptp10D[+]/Ptp4E¹/Ptp10D¹/Ptp4E[+]

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by CG32191^KK103889, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by CG12025^KK107653, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

NOT Enhanced by

Statement

Reference

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, non-enhanceable by Ptp10D[+]/Ptp10D¹

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has abnormal neuroanatomy | adult stage | progressive phenotype, non-enhanceable by Ptp4E¹/Ptp4E[+]

(Jansen et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by CG32191^GD6163, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by CG32191^GD6163, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by CG12025^GD2253, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by Ptp10D^KK101775, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by Ptp10D^KK101775, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by Ptp10D^GD115, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by Ptp10D^GD115, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by Ptp10D^GD2611, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by Ptp10D^GD2611, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by Vps13^HMS01715, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by Vps13^HMS01715, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by Vps13^HMS02460, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by Vps13^HMS02460, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by CG32191^KK103889, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by Ptp10D[+]/Ptp4E¹/Ptp10D¹/Ptp4E[+]

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by Ptp10D[+]/Ptp4E¹/Ptp10D¹/Ptp4E[+]

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by CG32191^KK103889, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by CG12025^KK107653, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, enhanceable by CG12025^KK107653, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, enhanceable by CG12025^GD2253, Scer\GAL4^ninaE.PT

(Jansen et al., 2017)

NOT Enhanced by

Statement

Reference

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, non-enhanceable by Ptp10D[+]/Ptp10D¹

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, non-enhanceable by Ptp10D[+]/Ptp10D¹

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has retina | adult stage | progressive phenotype, non-enhanceable by Ptp4E¹/Ptp4E[+]

(Jansen et al., 2017)

Hsap\SNCA^UAS.cJa, Scer\GAL4^ninaE.PT has ommatidium | adult stage | progressive phenotype, non-enhanceable by Ptp4E¹/Ptp4E[+]

(Jansen et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The age-progressive retinal degeneration characteristic for flies expressing Hsap\SNCA^Scer\UAS.cJa under the control of Scer\GAL4^ninaE.PT is significantly enhanced by co-expression of any of the following: CG32191^GD6163, CG32191^KK103889, CG12025^KK107653, CG12025^GD2253, Ptp10D^KK101775, Ptp10D^GD115, Ptp10D^GD2611, Vps13^HMS01715 and Vps13^HMS02460 or by combination with Ptp10D¹ and Ptp4E¹ in double heterozygous state, combination with single copy of each allele alone does not exacerbate the retinal phenotype.

(Jansen et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

51375

w¹¹¹⁸; P{UAS-SNCA.J}1/CyO

51376

w¹¹¹⁸; P{UAS-SNCA.J}7/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\SNCA^Scer\UAS.cJa

Hsap\SNCA^UAS.cJa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Jackson

Secondary FlyBase IDs

References (14)

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