Open Close
General Information
Symbol
Dmel\Npc1aKK101077
Species
D. melanogaster
Name
FlyBase ID
FBal0231055
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Npc1aKK101077 under the control of Scer\GAL4phm.PO (using tub-Gal80[ts] to limit the time of expression) results in accumulation of lipid droplets in the prothoracic gland cells of third instar larvae as well as trafficking defects as suggested by enlarged Rab7 positive vesicles and Lamp1 marker accumulations.

Without the tub-Gal80[ts] limiting the onset of expression the animals show developmental arrest in first larval instar.

Expression of Npc1aKK101077 RNAi under the Scer\GAL4P0206 driver results in a significant delay in pupariation and increased pupal size. Third instar larvae show accumulations of lipid droplets in prothoracic gland cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The L1 developmental arrest observed in animals expressing Npc1aKK101077 under the control of Scer\GAL4phm.PO is rescued by co-expression of sitScer\UAS.T:Ivir\HA1.

The accumulation of lipid droplets observed in prothoracic gland cells of third instar larvae expressing Npc1aKK101077 under the control of either Scer\GAL4phm.PO or Scer\GAL4P0206 is prevented by co-expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa.

The pupariation delay and increased pupal size observed in flies expressing Npc1aKK101077 under the Scer\GAL4P0206 driver is rescued by simultaneous co-expression of Atg1Scer\UAS.cSa and Atg13Scer\UAS.cCa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Npc1aKK101077
Name Synonyms
Secondary FlyBase IDs
    References (3)